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AB288377

Anti-Adenosine A1 Receptor antibody [10D12]

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Rabbit Recombinant Monoclonal Adenosine A1 Receptor antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ADORA1.

View Alternative Names

Adenosine receptor A1, ADORA1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody [10D12] (AB288377)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody [10D12] (AB288377)

IHC image of ab288377 diluted at 1 : 100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western blot - Anti-Adenosine A1 Receptor antibody [10D12] (AB288377)
  • WB

Supplier Data

Western blot - Anti-Adenosine A1 Receptor antibody [10D12] (AB288377)

All lanes:

Western blot - Anti-Adenosine A1 Receptor antibody [10D12] (ab288377) at 1/2000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

10D12

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ADORA1. The exact immunogen used to generate this antibody is proprietary information.

P30542

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Adenosine A1 Receptor also called A1 adenosine receptor is a G protein-coupled receptor (GPCR) with a mass of approximately 37 kDa. This receptor is primarily responsible for binding adenosine a nucleoside involved in numerous physiological processes. Mechanically the A1 receptor inhibits adenylyl cyclase activity via the Gi/o proteins resulting in decreased intracellular cyclic AMP levels. This receptor is expressed in various tissues including the brain heart and kidneys indicating its widespread physiological roles.
Biological function summary

The A1 receptor plays a significant role in mediating the inhibitory effects of adenosine on neurotransmitter release. It regulates synaptic transmission modulates excitability and protects tissues from excessive stimulation. This receptor does not function alone but is part of complex networks interacting with other proteins such as A2A receptors. A1 adenosine receptor signaling contributes to cardioprotection neuroprotection and regulation of renal blood flow reflecting its importance in maintaining homeostasis.

Pathways

The A1 receptor is essential in the adenosine signaling pathway and myocardial protection pathway. Through these pathways it influences important physiological responses like heart rate and blood flow. It interacts with proteins such as adenosine kinase and adenosine deaminase which regulate adenosine concentration influencing the availability of adenosine for receptor binding. The pathways involved illustrate the receptor's role in balancing energy supply and demand.

The A1 adenosine receptor is implicated in conditions like cardiac arrhythmias and epilepsy. Its role in reducing excitability and modulating synaptic transmission makes it a potential target for epilepsy treatment. In cardiac arrhythmias it helps regulate heart rate and protects against ischemic damage. The receptor's interactions with A2B receptors emphasize its role in complex disease mechanisms where it balances excitatory and inhibitory signals providing opportunities for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
See full target information ADORA1
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Product promise

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