Anti-Adenosine A1 Receptor antibody

• ICC

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Immunocytochemistry - Anti-Adenosine A1 Receptor antibody (AB3460)

Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized HeLa cells staining adenosine A1 receptor with ab3460 at 2 μg/ml and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei are stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin is stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control contains no primary anibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (AB3460)

ab3460 labelling Adenosine A1 receptor in the cytoplasm of Rat brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• WB

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Western blot - Anti-Adenosine A1 Receptor antibody (AB3460)

All lanes:

Western blot - Anti-Adenosine A1 Receptor antibody (ab3460) at 1/250 dilution

Lane 1:

PC-12 whole cell extract at 30 µg

Lane 2:

SH-SY5Y whole cell extract at 30 µg

Lane 3:

U-87 MG whole cell extract at 30 µg

Lane 4:

A549 whole cell extract at 30 µg

Lane 5:

K-562 whole cell extract at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate at 1/5000 dilution

Predicted band size: 37 kDa

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