Rabbit Polyclonal Adenosine A1 Receptor antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Mouse samples. Cited in 31 publications.
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- Animal nutrition (Zhongguo xu mu shou yi xue hui) 13:282-2882023Dietary adenosine supplementation improves placental angiogenesis in IUGR piglets by up-regulating adenosine A2a receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZifang Wu,Jiawei Nie,Deyuan Wu,Shuangbo Huang,Jianzhao Chen,Huajin Liang,Xiangyu Hao,Li Feng,Hefeng Luo,Chengquan TanPubMed 37168450
- Biomedicines 10:2022Adenosine Receptors Profile in Fibromuscular Dysplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaire Guiol,Sarah El Harake,Julien Fromonot,Mohamed Chefrour,Marguerite Gastaldi,Yassine Alibouch,Maxime Doublier,Pierre Deharo,Gabrielle Sarlon,Marion Marlinge,Nathalie Lalevee,Régis Guieu,François SilholPubMed 36359350
- Neural regeneration research 17:2504-25112022Silencing miRNA-324-3p protects against cerebral ischemic injury via regulation of the GATA2/A1R axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAn-Qi Zhang,Lu Wang,Yi-Xiu Wang,Shan-Shan Hong,Yu-Shan Zhong,Ru-Yi Yu,Xin-Lu Wu,Bing-Bing Zhou,Qi-Min Yu,Hai-Feng Fu,Shuang-Dong Chen,Yun-Chang Mo,Qin-Xue Dai,Jun-Lu WangPubMed 35535903
- Frontiers in cellular neuroscience 15:7834782021Cordycepin Ameliorates Synaptic Dysfunction and Dendrite Morphology Damage of Hippocampal CA1 A1R in Cerebral Ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao-Hui Chen,Yuan-Yuan Han,Ying-Jie Shang,Si-Yi Zhuang,Jun-Ni Huang,Bao-Yan Wu,Chu-Hua LiPubMed 35002628
- Frontiers in cardiovascular medicine 8:7611642021A Adenosine Receptor Subtypes Overproduction in Atria of Perioperative Atrial Fibrillation Patients Undergoing Cardiac Surgery: A Pilot Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaptiste Maille,Julien Fromonot,Claire Guiol,Marion Marlinge,Florian Baptiste,Suzy Lim,Charlotte Colombani,Marie Charlotte Chaptal,Mohamed Chefrour,Marguerite Gastaldi,Frederic Franceschi,Jean-Claude Deharo,Vlad Gariboldi,Jean Ruf,Giovanna Mottola,Régis GuieuPubMed 34805317
- Science (New York, N.Y.) 374:eabh28582021Astrocyte Ca-evoked ATP release regulates myelinated axon excitability and conduction speed.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Lezmy,I Lorena Arancibia-Cárcamo,Tania Quintela-López,Diane L Sherman,Peter J Brophy,David AttwellPubMed 34648330
- Life sciences 278:1195812021ADORA1 promotes nasopharyngeal carcinoma cell progression through regulation of PI3K/AKT/GSK-3β/β-catenin signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuming Pan,Sixian Liang,Xianyan WangPubMed 33961854
- eLife 10:2021Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDafeng Yang et. al.PubMed 33416495
- Molecular medicine reports 22:5348-53572020Inhibition of p38 MAPK regulates epileptic severity by decreasing expression levels of A1R and ENT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuejiao Zhou et. al.PubMed 33174009
- Aging cell 19:e132182020Extracellular adenosine signaling reverses the age-driven decline in the ability of neutrophils to kill Streptococcus pneumoniae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManmeet Bhalla et. al.PubMed 32790148
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Alternative names
Adenosine receptor A1
Recommended products
Rabbit Polyclonal Adenosine A1 Receptor antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Mouse samples. Cited in 31 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Replenishment batches of ab82477 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Adenosine A1 Receptor antibody (ab82477)
All lanes: Western blot - Anti-Adenosine A1 Receptor antibody (ab82477) at 1 µg/mL
Lane 1: Rat Spinal Ganglion Neuronal cell lysate at 10 µg
Lane 2: Western blot - EL4 whole cell lysate (EL4 whole cell lysate ab7183) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 118 kDa, 18 kDa, 36 kDa
Exposure time: 1min
Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab82477)
ICC/IF image of ab82477 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82477, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab82477)
IHC image of ab82477 staining in Rat Adult Brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82477, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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