JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317227

Anti-Adenosine A1 Receptor antibody [HL2442]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal Adenosine A1 Receptor antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Dog, Rat samples. Immunogen corresponding to Synthetic Peptide within Human ADORA1.

View Alternative Names

Adenosine receptor A1, ADORA1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)

ab317227 detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded rat cerebellum. Adenosine A1 Receptor stained by ab317227 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)
  • WB

Supplier Data

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)

Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 ug) were separated by 15% SDS-PAGE and the membranes were blotted with ab317227 diluted at 1 : 2000 and the highly cited competitor's diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (ab317227) at 1/2000 dilution

Lane 1:

Boiled SH-SY5Y whole cell extracts at 30 µg

Lane 2:

Boiled SH-SY5Y membrane extracts at 30 µg

Lane 3:

Unboiled SH-SY5Y whole cell extracts at 30 µg

Lane 4:

Unboiled SH-SY5Y membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)
  • WB

Supplier Data

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)

Non-transfected (–) and transfected (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317227 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (ab317227) at 1/1000 dilution

Lane 1:

LacZ shRNA transfected MCF-7 whole cell extract at 30 µg

Lane 2:

ADORA1 shRNA transfected MCF-7 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)
  • WB

Supplier Data

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 15% SDS-PAGE and the membrane was blotted with ab317227 diluted at 1 : 3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (ab317227) at 1/3000 dilution

Lane 1:

Non-transfected (–) HepG2 whole cell extracts at 30 µg

Lane 2:

ADORA1 shRNA transfected (+) HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)
  • WB

Supplier Data

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)

SH-SY5Y whole cell and membrane extracts (30 ug) were separated by 15% SDS-PAGE and the membrane was blotted with ab317227 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (ab317227) at 1/1000 dilution

Lane 1:

SH-SY5Y whole cell extracts at 30 µg

Lane 2:

SH-SY5Y membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)
  • WB

Supplier Data

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (AB317227)

Whole cell extract (30 ug) was separated by 15% SDS-PAGE and the membrane was blotted with ab317227 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Adenosine A1 Receptor antibody [HL2442] (ab317227) at 1/1000 dilution

All lanes:

MDCK whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2442

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Dog

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ADORA1.

P30542

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Adenosine A1 Receptor also called A1 adenosine receptor is a G protein-coupled receptor (GPCR) with a mass of approximately 37 kDa. This receptor is primarily responsible for binding adenosine a nucleoside involved in numerous physiological processes. Mechanically the A1 receptor inhibits adenylyl cyclase activity via the Gi/o proteins resulting in decreased intracellular cyclic AMP levels. This receptor is expressed in various tissues including the brain heart and kidneys indicating its widespread physiological roles.
Biological function summary

The A1 receptor plays a significant role in mediating the inhibitory effects of adenosine on neurotransmitter release. It regulates synaptic transmission modulates excitability and protects tissues from excessive stimulation. This receptor does not function alone but is part of complex networks interacting with other proteins such as A2A receptors. A1 adenosine receptor signaling contributes to cardioprotection neuroprotection and regulation of renal blood flow reflecting its importance in maintaining homeostasis.

Pathways

The A1 receptor is essential in the adenosine signaling pathway and myocardial protection pathway. Through these pathways it influences important physiological responses like heart rate and blood flow. It interacts with proteins such as adenosine kinase and adenosine deaminase which regulate adenosine concentration influencing the availability of adenosine for receptor binding. The pathways involved illustrate the receptor's role in balancing energy supply and demand.

The A1 adenosine receptor is implicated in conditions like cardiac arrhythmias and epilepsy. Its role in reducing excitability and modulating synaptic transmission makes it a potential target for epilepsy treatment. In cardiac arrhythmias it helps regulate heart rate and protects against ischemic damage. The receptor's interactions with A2B receptors emphasize its role in complex disease mechanisms where it balances excitatory and inhibitory signals providing opportunities for therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
See full target information ADORA1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com