AB197350

Anti-Adenosine A3 Receptor/A3AR antibody

Name: Anti-Adenosine A3 Receptor/A3AR antibody (ab197350) | Abcam
Brand: Abcam Limited
SKU: AB197350
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal Adenosine A3 Receptor/A3AR antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications.

View Alternative Names

Adenosine receptor A3, ADORA3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A3 Receptor/A3AR antibody (AB197350)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A3 Receptor/A3AR antibody (AB197350)

Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue sections labeling Adenosine A3 Receptor/A3AR with ab197350 at a 1/20 dilution.

Supplier Data

Western blot - Anti-Adenosine A3 Receptor/A3AR antibody (AB197350)

Gel : 10%SDS-PAGE

All lanes:

Western blot - Anti-Adenosine A3 Receptor/A3AR antibody (ab197350) at 1/100 dilution

All lanes:

mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Anti-rabbit IgG at 1/8000 dilution

Predicted band size: 36 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Adenosine A3 Receptor (A3AR) also known as A3 adenosine receptor is a member of the G-protein-coupled receptor family. This receptor couples with Gi proteins to exert its effects. The receptor has a molecular weight of approximately 37 kDa. It is expressed in a variety of tissues including the heart liver lung and immune system cells. A3AR's activity involves binding with adenosine molecules which modulates different cellular responses.

Biological function summary

This receptor mediates several processes by interacting with specific ligands. A3AR is not a part of a larger receptor complex but plays a significant role in modulating inflammation and immune response. The receptor contributes to processes like cell proliferation apoptosis and protection against tissue damage. Its activation generally promotes anti-inflammatory effects making it a target for therapeutic research.

Pathways

Many important biological activities are associated with the adenosine signaling pathway. A3AR plays an essential role in the regulation of the inflammatory response and the apoptosis pathway. Through these pathways it interacts with various proteins such as adenylyl cyclase which responds to ligand binding affecting cAMP levels in cells. The receptor's influence on immune cell function links it to other adenosine receptor subtypes.

Several conditions exhibit A3AR involvement such as rheumatoid arthritis and cancer. A3AR's anti-inflammatory and pro-apoptotic characteristics make it a point of interest for modulating immune-related disorders. Research also explores its connection with proteins like NF-κB due to their role in inflammation and cancer progression. Overall A3AR remains an active target for therapeutic strategies aiming to control these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Isoform 2. Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase (PubMed : 8234299).

See full target information ADORA3

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Purinergic signalling 20:371-384 PubMed37938538

2023

Modafinil exerts anti-inflammatory and anti-fibrotic effects by upregulating adenosine A and A receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Li,Ji Aee Kim,Seong-Eun Jo,Huisu Lee,Kwan-Chang Kim,Shinkyu Choi,Suk Hyo Suh

Animal nutrition (Zhongguo xu mu shou yi xue hui) 13:282-288 PubMed37168450

2023

Dietary adenosine supplementation improves placental angiogenesis in IUGR piglets by up-regulating adenosine A2a receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Zifang Wu,Jiawei Nie,Deyuan Wu,Shuangbo Huang,Jianzhao Chen,Huajin Liang,Xiangyu Hao,Li Feng,Hefeng Luo,Chengquan Tan

Biomedicines 10: PubMed35203424

2022

Expression of the Adenosine A-A Receptor Heteromer in Different Brain Regions and Marked Upregulation in the Microglia of the Transgenic APP Alzheimer's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Lillo,Iu Raïch,Jaume Lillo,Catalina Pérez-Olives,Gemma Navarro,Rafael Franco

The Tohoku journal of experimental medicine 249:275-283 PubMed31875581

2019

Adenosine Attenuates Aortic Smooth Muscle Cell Calcification through A Adenosine Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Fujimoto,Atsushi Shioi,Yuya Miki,Yoshinori Kakutani,Tomoaki Morioka,Tetsuo Shoji,Masanori Emoto,Masaaki Inaba

Translational oncology 11:552-558 PubMed29525632

2018

CLEC1B Expression and PD-L1 Expression Predict Clinical Outcome in Hepatocellular Carcinoma with Tumor Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Kuan Hu,Zhi-Ming Wang,Juan-Ni Li,Sai Zhang,Zhong-Fu Xiao,Yi-Ming Tao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Adenosine A3 Receptor/A3AR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A3 Receptor/A3AR antibody (AB197350)

Western blot - Anti-Adenosine A3 Receptor/A3AR antibody (AB197350)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise