JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB140604

Anti-Adenosine phosphate antibody [EPR8471]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Adenosine phosphate antibody. Suitable for ELISA and reacts with Chemical samples. Cited in 1 publication.

View Alternative Names

ADP, AMP, ATP, Adenosine diphosphate, Cyclic adenosine-3閳, 5閳-monophosphate, adenosine monophosphate, adenosine triphosphate

1 Images
ELISA - Anti-Adenosine phosphate antibody [EPR8471] (AB140604)
  • ELISA

Unknown

ELISA - Anti-Adenosine phosphate antibody [EPR8471] (AB140604)

50 µl of 0.1ug/ml of cAMP-SPDP-BSA was coated into 96-wells. After blocking with 1% BSA, 25 µl of 5 mg/ml of cAMP, 5’-AMP, 5’-AMP-Na2, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, or H2O and 25 µl of ab140604 at 0.5 ug/ml were added to 96-wells and incubated for 30 min on a shaker. After washing, HRP conjugated goat anti-rabbit IgG Fc specific antibody was added and incubated. After washing, TMB substrate was used to develop color.

  • Carrier free

    Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8471

Isotype

IgG

Carrier free

No

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab140604 recognizes cAMP, AMP, ADP, and ATP only. It has less cross-reactivity with cGMP, GMP, GDP,GTP, and Adenosine.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.1 µg/mL", "ELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine phosphate commonly referred to as adenine nucleotides includes compounds like adenosine monophosphate (AMP) adenosine diphosphate (ADP) and adenosine triphosphate (ATP). These molecules have important roles in energy transfer and storage. All types of cells express adenosine phosphates reflecting its significance in cellular activities. The molecular weight can vary depending on the number of phosphate groups: for example ATP which is the most well-known form typically has a mass of approximately 507.18 g/mol.
Biological function summary

Adenosine phosphates serve as energy currency within the cell. They do not generally exist in isolation but are part of larger complexes and biochemical structures. For instance ATP is particularly known for its role in coupling and driving endergonic reactions ensuring cells receive the necessary energy for various processes. When hydrolyzed ATP releases a significant amount of energy which is then harnessed by cellular machinery to perform tasks like biosynthesis motility and division.

Pathways

The role of adenosine phosphates is central to cellular respiration and metabolism. The oxidative phosphorylation pathway within mitochondria is a critical process involving ATP synthesis. Related proteins include various subunits of ATP synthase which play roles in converting ADP to ATP. Another relevant pathway is glycolysis where ADP and ATP are key players in the breakdown of glucose to provide energy with enzymes like phosphofructokinase aiding the transformation processes.

Its dysregulation or imbalance is linked to conditions such as mitochondrial diseases and ischemic events. Mitochondrial diseases frequently result from impaired energy production involving proteins like mitochondrial ATP synthase. Meanwhile ischemic conditions connecting to disrupted ATP supply can exacerbate symptoms or lead to further cellular damage. Understanding the role of adenosine phosphates in these disorders can guide research for potential therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Adenosine phosphate
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pediatric blood & cancer 71:e30717 PubMed37814421

2023

Immunohistochemical expression of TFF1 is a marker of poor prognosis in retinoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rosario Aschero,Daiana Ganiewich,Gabriela Lamas,Camilo A Restrepo-Perdomo,Daniela Ottaviani,Santiago Zugbi,Sandra Camarero,Ezequiel Néspoli,Maria Cuadrado Vilanova,Sara Perez-Jaume,Guillem Pascual-Pasto,Claudia Sampor,Nathalia Grigorovski,Beatriz Salas,Mariona Suñol,Angel M Carcaboso,Jaume Mora,María T G de Dávila,François Doz,François Radvanyi,David H Abramson,Andrea S Llera,Paula S Schaiquevich,Fabiana Lubieniecki,Guillermo L Chantada
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB228606

Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free

RabMAb|Recombinant

ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (AB228606)

  • 0
  • 0
Assay Kits

AB253209

Rat CD73 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Rat CD73 ELISA Kit (AB253209)

  • 0
  • 0
Primary Antibodies

AB302714

Anti-SPHK1 antibody [EPR24511-37]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-SPHK1 antibody [EPR24511-37] (AB302714)

  • 0
  • 0
Primary Antibodies

AB140592

Anti-S1P antibody

Western blot - Anti-S1P antibody (AB140592)

  • 3
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com