Rabbit Recombinant Monoclonal Adenosine phosphate antibody. Carrier free. Suitable for ELISA and reacts with Chemical samples.
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![ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (AB228606), expandable thumbnail](https://content.abcam.com/products/images/adenosine-phosphate-antibody-epr8471-bsa-and-azide-free-ab228606--elisa-img415636.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (AB228606)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ELISA | |
|---|---|
| Chemical | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Alternative names
ADP, AMP, ATP, Adenosine diphosphate, Cyclic adenosine-3閳, 5閳-monophosphate, adenosine monophosphate, adenosine triphosphate
Recommended products
Rabbit Recombinant Monoclonal Adenosine phosphate antibody. Carrier free. Suitable for ELISA and reacts with Chemical samples.
Alternative names
ADP, AMP, ATP, Adenosine diphosphate, Cyclic adenosine-3閳, 5閳-monophosphate, adenosine monophosphate, adenosine triphosphate
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR8471
- Purification technique
Affinity purification Protein A
- Specificity
This antibody recognizes cAMP, AMP, ADP, and ATP only. It has less cross-reactivity with cGMP, GMP, GDP,GTP, and Adenosine.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab228606 is the carrier-free version of Anti-Adenosine phosphate antibody [EPR8471] ab140604.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adenosine phosphate commonly referred to as adenine nucleotides includes compounds like adenosine monophosphate (AMP) adenosine diphosphate (ADP) and adenosine triphosphate (ATP). These molecules have important roles in energy transfer and storage. All types of cells express adenosine phosphates reflecting its significance in cellular activities. The molecular weight can vary depending on the number of phosphate groups: for example ATP which is the most well-known form typically has a mass of approximately 507.18 g/mol.
- Biological function summary
Adenosine phosphates serve as energy currency within the cell. They do not generally exist in isolation but are part of larger complexes and biochemical structures. For instance ATP is particularly known for its role in coupling and driving endergonic reactions ensuring cells receive the necessary energy for various processes. When hydrolyzed ATP releases a significant amount of energy which is then harnessed by cellular machinery to perform tasks like biosynthesis motility and division.
- Pathways
The role of adenosine phosphates is central to cellular respiration and metabolism. The oxidative phosphorylation pathway within mitochondria is a critical process involving ATP synthesis. Related proteins include various subunits of ATP synthase which play roles in converting ADP to ATP. Another relevant pathway is glycolysis where ADP and ATP are key players in the breakdown of glucose to provide energy with enzymes like phosphofructokinase aiding the transformation processes.
- Associated diseases and disorders
Its dysregulation or imbalance is linked to conditions such as mitochondrial diseases and ischemic events. Mitochondrial diseases frequently result from impaired energy production involving proteins like mitochondrial ATP synthase. Meanwhile ischemic conditions connecting to disrupted ATP supply can exacerbate symptoms or lead to further cellular damage. Understanding the role of adenosine phosphates in these disorders can guide research for potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (ab228606), expandable thumbnail](https://content.abcam.com/products/images/adenosine-phosphate-antibody-epr8471-bsa-and-azide-free-ab228606--elisa-img415636.jpg "ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (ab228606)")
ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (ab228606)
This data was developed using Anti-Adenosine phosphate antibody [EPR8471] ab140604, the same antibody clone in a different buffer formulation.50 μl of 0.1μg/ml of cAMP-SPDP-BSA was coated into 96-wells. After blocking with 1% BSA, 25 μl of 5 mg/ml of cAMP, 5'-AMP, 5'-AMP-Na2, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, or H2O and 25 μl of Anti-Adenosine phosphate antibody [EPR8471] ab140604 at 0.5 μg/ml were added to 96-wells and incubated for 30 min on a shaker. After washing, HRP conjugated goat anti-rabbit IgG Fc specific antibody was added and incubated. After washing, TMB substrate was used to develop color.
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