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AB228606

Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free

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Rabbit Recombinant Monoclonal Adenosine phosphate antibody. Carrier free. Suitable for ELISA and reacts with Chemical samples.
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ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (AB228606)
  • ELISA

Unknown

ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (AB228606)

This data was developed using ab140604, the same antibody clone in a different buffer formulation.50 μl of 0.1μg/ml of cAMP-SPDP-BSA was coated into 96-wells. After blocking with 1% BSA, 25 μl of 5 mg/ml of cAMP, 5'-AMP, 5'-AMP-Na2, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, or H2O and 25 μl of ab140604 at 0.5 μg/ml were added to 96-wells and incubated for 30 min on a shaker. After washing, HRP conjugated goat anti-rabbit IgG Fc specific antibody was added and incubated. After washing, TMB substrate was used to develop color.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8471

Isotype

IgG

Carrier free

Yes

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes cAMP, AMP, ADP, and ATP only. It has less cross-reactivity with cGMP, GMP, GDP,GTP, and Adenosine.

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Complementary Products

Primary Antibodies

AB140604

Anti-Adenosine phosphate antibody [EPR8471]

RabMAb|Recombinant

ELISA - Anti-Adenosine phosphate antibody [EPR8471] (AB140604)

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Assay Kits

AB211094

Adenosine Assay Kit (Fluorometric)

Functional Studies - Adenosine Assay Kit (Fluorometric) (AB211094)

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Primary Antibodies

AB249503

Anti-cGMP antibody [EPR10355] - BSA and Azide free

RabMAb|Recombinant

Competitive ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (AB249503)

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  • 0
Assay Kits

AB204695

Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric)

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)

  • 4
  • 1
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Reactivity data

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Product details

ab228606 is the carrier-free version of ab140604.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine phosphate commonly referred to as adenine nucleotides includes compounds like adenosine monophosphate (AMP) adenosine diphosphate (ADP) and adenosine triphosphate (ATP). These molecules have important roles in energy transfer and storage. All types of cells express adenosine phosphates reflecting its significance in cellular activities. The molecular weight can vary depending on the number of phosphate groups: for example ATP which is the most well-known form typically has a mass of approximately 507.18 g/mol.
Biological function summary

Adenosine phosphates serve as energy currency within the cell. They do not generally exist in isolation but are part of larger complexes and biochemical structures. For instance ATP is particularly known for its role in coupling and driving endergonic reactions ensuring cells receive the necessary energy for various processes. When hydrolyzed ATP releases a significant amount of energy which is then harnessed by cellular machinery to perform tasks like biosynthesis motility and division.

Pathways

The role of adenosine phosphates is central to cellular respiration and metabolism. The oxidative phosphorylation pathway within mitochondria is a critical process involving ATP synthesis. Related proteins include various subunits of ATP synthase which play roles in converting ADP to ATP. Another relevant pathway is glycolysis where ADP and ATP are key players in the breakdown of glucose to provide energy with enzymes like phosphofructokinase aiding the transformation processes.

Its dysregulation or imbalance is linked to conditions such as mitochondrial diseases and ischemic events. Mitochondrial diseases frequently result from impaired energy production involving proteins like mitochondrial ATP synthase. Meanwhile ischemic conditions connecting to disrupted ATP supply can exacerbate symptoms or lead to further cellular damage. Understanding the role of adenosine phosphates in these disorders can guide research for potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Adenosine phosphate
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com