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Mouse Monoclonal Adenosine Receptor A2a antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (AB79714), expandable thumbnail
  • Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (AB79714), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (AB79714), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (AB79714), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Tested
Tested
Not recommended
Dog
Predicted
Predicted
Not recommended
Guinea pig
Predicted
Predicted
Not recommended
Hamster
Predicted
Predicted
Not recommended
Primates
Predicted
Predicted
Not recommended

Tested
Tested

Species

Mouse

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Guinea pig, Hamster, Dog, Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1.00000-5.00000 µg/mL

Notes

We recommend using 1% BSA as a blocking agent for western blot.

Species

Rat

Dilution info

1.00000-5.00000 µg/mL

Notes

We recommend using 1% BSA as a blocking agent for western blot.

Species

Human

Dilution info

1.00000-5.00000 µg/mL

Notes

We recommend using 1% BSA as a blocking agent for western blot.

Predicted
Predicted

Species

Guinea pig, Hamster, Dog, Primates

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Guinea pig, Hamster, Dog, Primates

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).

Alternative names

Adenosine receptor A2a, ADORA2A, ADORA2

Recommended products

Mouse Monoclonal Adenosine Receptor A2a antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

7F6-G5-A2

Purification technique

Affinity purification Protein G

Specificity

This antibody is recommended for tissue lysates only. In house testing has shown no signal in Western Blot for SH-SY5Y, SK-N-SH, PC-12 or HeLa cell lines.

Epitope

Recognises amino acids 213-220 (SQPLPGER) within the third intracellular loop.

Concentration
Loading...
Purification notes

Purified by running the antiserum from the injected animal through an affinity column with the antigen bound to a beaded agarose gel.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Adenosine Receptor A2A also known as A2AR is a G protein-coupled receptor with a molecular mass of approximately 45 kDa. This receptor is widely expressed in various body tissues including the brain heart spleen and immune cells. A2A receptors play a mechanical role in mediating physiological responses to adenosine a purine nucleoside that acts as a signaling molecule in the body. Through binding to adenosine A2A receptors activate intracellular pathways that modulate cellular responses.

Biological function summary

The A2A adenosine receptor functions as an important regulator of neurotransmission and immune response. It does not form part of a larger protein complex but rather independently influences processes such as vasodilation heart rate regulation and cytokine release. The A2A receptor contributes to the modulation of dopamine and glutamate signaling in the central nervous system playing a significant role in maintaining neural balance. Its activity in immune cells helps in controlling inflammation and immune cell proliferation.

Pathways

The A2A adenosine receptor engages in critical signaling cascades particularly the cAMP-PKA pathway and the MAPK pathway. Through the cAMP-PKA pathway A2A R influences various cellular processes by increasing cyclic AMP levels which activate protein kinase A. The receptor is also involved in the MAPK pathway impacting cellular growth and differentiation. It interacts with proteins such as Gs alpha subunit which couples to the receptor to promote downstream signaling events.

Associated diseases and disorders

The A2A receptor has connections to conditions like Parkinson's disease and ischemia-reperfusion injury. In Parkinson's disease the activity of A2A receptors affects dopaminergic signaling where it interacts with proteins such as dopamine D2 receptors suggesting potential for therapeutic targeting. During ischemia-reperfusion injury the receptor's modulation of immune responses and vasodilation can influence tissue damage and repair mechanisms linking it with proteins involved in inflammatory pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

    IHC image of Adenosine Receptor A2a staining in Mouse normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714), expandable thumbnail

    Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

    Blocking buffer: 1% BSA

    Gel type: MOPS

    Lanes 1 - 3: Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) at 5 µg/mL

    Lanes 4 - 6: Competitor product at 5 µg/mL

    Lanes 1 and 4: Human brain tissue lysate at 20 µg

    Lanes 2 and 5: Mouse brain tissue lysate at 20 µg

    Lanes 3 and 6: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Predicted band size: 44 kDa

    Observed band size: 25 kDa, 39 kDa, 45 kDa, 55 kDa

    Exposure time: 20min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

    IHC image of Adenosine Receptor A2a staining in Human normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

    IHC image of Adenosine Receptor A2a staining in Rat normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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