Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]

4 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

  IHC image of Adenosine Receptor A2a staining in Mouse normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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  Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

  Adenosine Receptor A2a Western blot staining using mouse Anti-Adenosine Receptor A2a antibody

  Blocking buffer: 1% BSA
  

  Gel type: MOPS

  Lanes 1 - 3: Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) at 5 µg/mL

  Lanes 4 - 6: Competitor product at 5 µg/mL

  Lanes 1 and 4: Human brain tissue lysate at 20 µg

  Lanes 2 and 5: Mouse brain tissue lysate at 20 µg

  Lanes 3 and 6: Rat brain tissue lysate at 20 µg

  Secondary

  All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

  Predicted band size: 44 kDa

  Observed band size: 25 kDa, 39 kDa, 45 kDa, 55 kDa

  Exposure time: 20min

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

  IHC image of Adenosine Receptor A2a staining in Human normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

  IHC image of Adenosine Receptor A2a staining in Rat normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.