AB101678

Anti-Adenosine Receptor A2a antibody

Name: Anti-Adenosine Receptor A2a antibody (ab101678) | Abcam
Brand: Abcam Limited
SKU: AB101678
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Adenosine Receptor A2a antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADORA2A aa 250 to C-terminus.

View Alternative Names

ADORA2, ADORA2A, Adenosine receptor A2a

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB101678)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB101678)

ab101678, at 1/100 dilution, staining membrane Adenosine Receptor A2a in formalin-fixed, paraffin-embedded Human fetal colon tissue, by Immunohistochemistry.

Unknown

Western blot - Anti-Adenosine Receptor A2a antibody (AB101678)

All lanes:

Western blot - Anti-Adenosine Receptor A2a antibody (ab101678) at 1/500 dilution

All lanes:

Human fetal spleen lysate

Predicted band size: 44 kDa

Observed band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ADORA2A aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine Receptor A2A also known as A2AR is a G protein-coupled receptor with a molecular mass of approximately 45 kDa. This receptor is widely expressed in various body tissues including the brain heart spleen and immune cells. A2A receptors play a mechanical role in mediating physiological responses to adenosine a purine nucleoside that acts as a signaling molecule in the body. Through binding to adenosine A2A receptors activate intracellular pathways that modulate cellular responses.

Biological function summary

The A2A adenosine receptor functions as an important regulator of neurotransmission and immune response. It does not form part of a larger protein complex but rather independently influences processes such as vasodilation heart rate regulation and cytokine release. The A2A receptor contributes to the modulation of dopamine and glutamate signaling in the central nervous system playing a significant role in maintaining neural balance. Its activity in immune cells helps in controlling inflammation and immune cell proliferation.

Pathways

The A2A adenosine receptor engages in critical signaling cascades particularly the cAMP-PKA pathway and the MAPK pathway. Through the cAMP-PKA pathway A2A R influences various cellular processes by increasing cyclic AMP levels which activate protein kinase A. The receptor is also involved in the MAPK pathway impacting cellular growth and differentiation. It interacts with proteins such as Gs alpha subunit which couples to the receptor to promote downstream signaling events.

The A2A receptor has connections to conditions like Parkinson's disease and ischemia-reperfusion injury. In Parkinson's disease the activity of A2A receptors affects dopaminergic signaling where it interacts with proteins such as dopamine D2 receptors suggesting potential for therapeutic targeting. During ischemia-reperfusion injury the receptor's modulation of immune responses and vasodilation can influence tissue damage and repair mechanisms linking it with proteins involved in inflammatory pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).

See full target information ADORA2A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical endocrinology and metaboli 98:E1345-51 PubMed23788688

2013

Inhibition of parathyroid hormone secretion by caffeine in human parathyroid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Lu,Lars-Ove Farnebo,Robert Bränström,Catharina Larsson

Journal of cellular physiology 228:602-8 PubMed22833450

2012

5'-ectonucleotidase mediates multiple-drug resistance in glioblastoma multiforme cells.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Quezada,Wallys Garrido,Carlos Oyarzún,Katia Fernández,Rodrigo Segura,Rómulo Melo,Paola Casanello,Luis Sobrevia,Rody San Martín

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Adenosine Receptor A2a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB101678)

Western blot - Anti-Adenosine Receptor A2a antibody (AB101678)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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