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Rabbit Recombinant Monoclonal Adenosine Receptor A2a antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB260032), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB260032), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB260032), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB260032), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB260032), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Rat
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).

Alternative names

Adenosine receptor A2a, ADORA2A, ADORA2

Recommended products

Rabbit Recombinant Monoclonal Adenosine Receptor A2a antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Alternative names

Adenosine receptor A2a, ADORA2A, ADORA2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23731-50

Purification technique

Affinity purification Protein A

Specificity

Expression levels of the target protein vary with sample type and optimisation may be required. In our hands we are only able to detect ADORA2A in relevant neurological samples and do not recommend this product for use in other samples.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Adenosine Receptor A2A also known as A2AR is a G protein-coupled receptor with a molecular mass of approximately 45 kDa. This receptor is widely expressed in various body tissues including the brain heart spleen and immune cells. A2A receptors play a mechanical role in mediating physiological responses to adenosine a purine nucleoside that acts as a signaling molecule in the body. Through binding to adenosine A2A receptors activate intracellular pathways that modulate cellular responses.

Biological function summary

The A2A adenosine receptor functions as an important regulator of neurotransmission and immune response. It does not form part of a larger protein complex but rather independently influences processes such as vasodilation heart rate regulation and cytokine release. The A2A receptor contributes to the modulation of dopamine and glutamate signaling in the central nervous system playing a significant role in maintaining neural balance. Its activity in immune cells helps in controlling inflammation and immune cell proliferation.

Pathways

The A2A adenosine receptor engages in critical signaling cascades particularly the cAMP-PKA pathway and the MAPK pathway. Through the cAMP-PKA pathway A2A R influences various cellular processes by increasing cyclic AMP levels which activate protein kinase A. The receptor is also involved in the MAPK pathway impacting cellular growth and differentiation. It interacts with proteins such as Gs alpha subunit which couples to the receptor to promote downstream signaling events.

Associated diseases and disorders

The A2A receptor has connections to conditions like Parkinson's disease and ischemia-reperfusion injury. In Parkinson's disease the activity of A2A receptors affects dopaminergic signaling where it interacts with proteins such as dopamine D2 receptors suggesting potential for therapeutic targeting. During ischemia-reperfusion injury the receptor's modulation of immune responses and vasodilation can influence tissue damage and repair mechanisms linking it with proteins involved in inflammatory pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded human cerebrum (striatum) tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebrum is observed. The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilutionfollowed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the striatum of the mouse cerebrum (PMID: 21858817). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: No staining observed on human diffuse large B-cell lymphoma tissue .

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: No staining observed on human hepatocellular carcinoma tissue.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: No staining observed on human spleen tissue.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: No staining observed on human thymoma tissue.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Tissue Microarrays stained for "Anti-Adenosine Receptor A2a antibody [EPR23731-50]" using "ab260032"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab260032 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)

    Tissue Microarrays stained for "Anti-Adenosine Receptor A2a antibody [EPR23731-50]" using "ab260032"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab260032 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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