Rabbit Recombinant Monoclonal Adenosine Receptor A2a antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Human, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172400.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68415.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8556.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24343.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4309.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (AB273879)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Alternative names
Adenosine receptor A2a, ADORA2A, ADORA2
Recommended products
Rabbit Recombinant Monoclonal Adenosine Receptor A2a antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23731-50
- Purification technique
Affinity purification Protein A
- Specificity
Expression levels of the target protein vary with sample type and optimisation may be required. In our hands we are only able to detect ADORA2A in relevant neurological samples and do not recommend this product for use in other samples.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab273879 is the carrier-free version of Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adenosine Receptor A2A also known as A2AR is a G protein-coupled receptor with a molecular mass of approximately 45 kDa. This receptor is widely expressed in various body tissues including the brain heart spleen and immune cells. A2A receptors play a mechanical role in mediating physiological responses to adenosine a purine nucleoside that acts as a signaling molecule in the body. Through binding to adenosine A2A receptors activate intracellular pathways that modulate cellular responses.
- Biological function summary
The A2A adenosine receptor functions as an important regulator of neurotransmission and immune response. It does not form part of a larger protein complex but rather independently influences processes such as vasodilation heart rate regulation and cytokine release. The A2A receptor contributes to the modulation of dopamine and glutamate signaling in the central nervous system playing a significant role in maintaining neural balance. Its activity in immune cells helps in controlling inflammation and immune cell proliferation.
- Pathways
The A2A adenosine receptor engages in critical signaling cascades particularly the cAMP-PKA pathway and the MAPK pathway. Through the cAMP-PKA pathway A2A R influences various cellular processes by increasing cyclic AMP levels which activate protein kinase A. The receptor is also involved in the MAPK pathway impacting cellular growth and differentiation. It interacts with proteins such as Gs alpha subunit which couples to the receptor to promote downstream signaling events.
- Associated diseases and disorders
The A2A receptor has connections to conditions like Parkinson's disease and ischemia-reperfusion injury. In Parkinson's disease the activity of A2A receptors affects dopaminergic signaling where it interacts with proteins such as dopamine D2 receptors suggesting potential for therapeutic targeting. During ischemia-reperfusion injury the receptor's modulation of immune responses and vasodilation can influence tissue damage and repair mechanisms linking it with proteins involved in inflammatory pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172400.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded human cerebrum (striatum) tissue labeling Adenosine receptor A2a with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebrum is observed. The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68415.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8556.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilutionfollowed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the striatum of the mouse cerebrum (PMID: 21858817). The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24343.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4309.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169532.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling Adenosine receptor A2a with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Negative control: No staining observed on human diffuse large B-cell lymphoma tissue .
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164749.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Adenosine receptor A2a with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Negative control: No staining observed on human hepatocellular carcinoma tissue.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180740.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Adenosine receptor A2a with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Negative control: No staining observed on human spleen tissue.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170357.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling Adenosine receptor A2a with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The section was incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Negative control: No staining observed on human thymoma tissue.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423102.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Tissue Microarrays stained for "Anti-Adenosine Receptor A2a antibody [EPR23731-50]" using "Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423101.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
Tissue Microarrays stained for "Anti-Adenosine Receptor A2a antibody [EPR23731-50]" using "Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with Anti-Adenosine Receptor A2a antibody [EPR23731-50] ab260032 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169532.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164749.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180740.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170357.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423102.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879), expandable thumbnail](https://content.abcam.com/products/images/adenosine-receptor-a2a-antibody-epr23731-50-bsa-and-azide-free-ab273879--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423101.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)")
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