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AB111903

Anti-Adenosyl Homocysteine antibody [EPR4499]

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(1 Publication)

Rabbit Recombinant Monoclonal Adenosyl Homocysteine antibody. Suitable for cELISA and reacts with Modified Amino Acid samples. Cited in 1 publication.

View Alternative Names

S (5' Adenosyl) L homocysteine, S Adenosyl L homocysteine, S adenosylhomocysteine, SAH

1 Images
Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] (AB111903)
  • cELISA

Unknown

Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] (AB111903)

Competitive ELISA : 0.1 μg/ml of BSA-S-adenosylhomocysteine was coated into 96 wells. Serial dilution of S-adenosylhomocysteine (SAH), SAMe, et al. and 0.05 μg/ml of ab111903 were added. HRP conjugated Goat anti-Rabbit IgG antibody was used to develop the color.

  • Carrier free

    Anti-Adenosyl Homocysteine antibody [EPR4499] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4499

Isotype

IgG

Carrier free

No

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody shows the following reactivities with related compounds:S-(5'-Adenosyl)-L-homocysteine: 100%S-(5'-Adenosyl)-L-methionine: 0%Adenosine: 4.4%Homocysteine: 4.4%Cystathionine: 0%L-Cysteine: 1.5%Glutathione: 1.8%

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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB168537

Anti-Adenosyl Homocysteine antibody [EPR4499] - BSA and Azide free

RabMAb|Recombinant

Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] - BSA and Azide free (AB168537)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "1/5000", "cELISA-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosyl Homocysteine is a critical intermediate in the biological process known as the methionine cycle. It is also referred to as S-Adenosylhomocysteine (SAH) with a molecular mass of approximately 384.46 g/mol. This compound forms as a by-product of methylation reactions after the methyl group transfer from S-Adenosylmethionine (SAM). Adenosyl Homocysteine hydrolase further processes it into homocysteine and adenosine. Expression of Adenosyl Homocysteine is ubiquitous occurring across various cell types and tissues in the body.
Biological function summary

Adenosyl homocysteine plays an important role in regulating methylation processes which are essential for various cellular functions like epigenetic modifications and gene expression regulation. It forms part of a complex network where it acts as a metabolic regulator. By inhibiting methyltransferase enzymes it directly controls the levels of SAM affecting the methylation status in cells. As a feedback inhibitor Adenosyl Homocysteine balances methyl group transfers critical for cellular function.

Pathways

Adenosyl homocysteine sits at the intersection of the methionine and homocysteine metabolic pathways. It maintains a delicate balance necessary for transmethylation reactions. The methionine cycle and homocysteine metabolism pathways are linked by this compound. Adenosyl Homocysteine connects with other proteins such as S-Adenosylhomocysteine hydrolase and methionine synthase which are important for transferring methyl groups important for cellular processes.

Disruptions in Adenosyl Homocysteine levels contribute to conditions like cardiovascular diseases and hyperhomocysteinemia. Elevated levels of homocysteine resulting from inefficient conversion of Adenosyl Homocysteine are associated with these disorders. Within these diseases adenosyl homocysteine relates to proteins like homocysteine methyltransferase that influence methionine metabolism. The imbalance involves increased risk factors for vascular diseases establishing Adenosyl Homocysteine as a target for therapeutic intervention and research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Adenosyl Homocysteine
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 18:13020-6 PubMed24145794

2013

Selection of a mimotope peptide of S-adenosyl-L-homocysteine and its application in immunoassays.

Applications

ELISA

Species

Unspecified reactive species

Chun Wu,George Tzertzinis
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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