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AB168537

Anti-Adenosyl Homocysteine antibody [EPR4499] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Adenosyl Homocysteine antibody. Carrier free. Suitable for cELISA and reacts with Modified Amino Acid samples. Cited in 1 publication.

View Alternative Names

SAHH, AHCY, Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, S (5' Adenosyl) L homocysteine, S Adenosyl L homocysteine, S adenosylhomocysteine, SAH

1 Images
Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] - BSA and Azide free (AB168537)
  • cELISA

Unknown

Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] - BSA and Azide free (AB168537)

This ELISA data was generated using the same anti-Adenosyl Homocysteine clone, EPR4499, in a different buffer formulation (cat# ab111903).

Competitive ELISA : 0.1 μg/ml of BSA-S-adenosylhomocysteine was coated into 96 wells. Serial dilution of S-adenosylhomocysteine (SAH), SAMe, et al. and 0.05 μg/ml of ab111903 were added. HRP conjugated Goat anti-Rabbit IgG antibody was used to develop the color.)

  • Unconjugated

    Anti-Adenosyl Homocysteine antibody [EPR4499]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4499

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody shows the following reactivities with related compounds:S-(5'-Adenosyl)-L-homocysteine: 100%S-(5'-Adenosyl)-L-methionine: 0%Adenosine: 4.4%Homocysteine: 4.4%Cystathionine: 0%L-Cysteine: 1.5%Glutathione: 1.8%

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Complementary Products

Assay Kits

AB309446

Human SAHH ELISA Kit

Sandwich ELISA - Human SAHH ELISA Kit (AB309446)

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Primary Antibodies

AB111903

Anti-Adenosyl Homocysteine antibody [EPR4499]

RabMAb|Recombinant

Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] (AB111903)

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Primary Antibodies

AB238682

Anti-ACSM3/SAH antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSM3/SAH antibody (AB238682)

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Proteins & Peptides

AB157892

Recombinant Human Acyloxyacyl Hydrolase protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Acyloxyacyl Hydrolase protein (GST tag N-Terminus) (AB157892)

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Primary Antibodies

AB129106

Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (AB129106)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }
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Product details

ab168537 is the carrier-free version of ab111903.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosyl Homocysteine is a critical intermediate in the biological process known as the methionine cycle. It is also referred to as S-Adenosylhomocysteine (SAH) with a molecular mass of approximately 384.46 g/mol. This compound forms as a by-product of methylation reactions after the methyl group transfer from S-Adenosylmethionine (SAM). Adenosyl Homocysteine hydrolase further processes it into homocysteine and adenosine. Expression of Adenosyl Homocysteine is ubiquitous occurring across various cell types and tissues in the body.
Biological function summary

Adenosyl homocysteine plays an important role in regulating methylation processes which are essential for various cellular functions like epigenetic modifications and gene expression regulation. It forms part of a complex network where it acts as a metabolic regulator. By inhibiting methyltransferase enzymes it directly controls the levels of SAM affecting the methylation status in cells. As a feedback inhibitor Adenosyl Homocysteine balances methyl group transfers critical for cellular function.

Pathways

Adenosyl homocysteine sits at the intersection of the methionine and homocysteine metabolic pathways. It maintains a delicate balance necessary for transmethylation reactions. The methionine cycle and homocysteine metabolism pathways are linked by this compound. Adenosyl Homocysteine connects with other proteins such as S-Adenosylhomocysteine hydrolase and methionine synthase which are important for transferring methyl groups important for cellular processes.

Disruptions in Adenosyl Homocysteine levels contribute to conditions like cardiovascular diseases and hyperhomocysteinemia. Elevated levels of homocysteine resulting from inefficient conversion of Adenosyl Homocysteine are associated with these disorders. Within these diseases adenosyl homocysteine relates to proteins like homocysteine methyltransferase that influence methionine metabolism. The imbalance involves increased risk factors for vascular diseases establishing Adenosyl Homocysteine as a target for therapeutic intervention and research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).
See full target information AHCY

Additional targets

Adenosyl Homocysteine
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed33499026

2021

Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Flores-Muñoz,Jaime Maripillán,Jacqueline Vásquez-Navarrete,Joel Novoa-Molina,Ricardo Ceriani,Helmuth A Sánchez,Ana C Abbott,Caroline Weinstein-Oppenheimer,Donald I Brown,Ana María Cárdenas,Isaac E García,Agustín D Martínez
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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