Anti-Adenovirus Type 5 antibody

• WB

AbReview59142****

Western blot - Anti-Adenovirus Type 5 antibody (AB6982)

Blocked with 5% milk

All lanes:

Western blot - Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

Lane 1:

human hepatocytes whole cell lysate at 20 µg

Lane 2:

human hepatocytes infected with Adv-iNOS whole cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution

Observed band size: 130 kDa

true

Exposure time: 20s

Image courtesy of an anonymous Abreview

• ICC/IF

AbReview18561****

Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (AB6982)

ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.

Image courtesy of an anonymous Abreview.

• WB

AbReview22715****

Western blot - Anti-Adenovirus Type 5 antibody (AB6982)

Blocking step was performed using 5% milk for 1 hour at 23°C.

All lanes:

Western blot - Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

Lane 1:

Whole cell lysate prepared from U2OS cells. at 35 µg

Lane 2:

Whole cell lysate prepared from U2OS cells infected with Adenovirus 5. at 35 µg

Secondary

All lanes:

Goat anti-rabbit conjugated to IRDye 700DX

false

Image courtesy of an anonymous Abreview.

• WB

Unknown

Western blot - Anti-Adenovirus Type 5 antibody (AB6982)

Top blot (A) : ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).

Bottom blot (B) : As above, except ab6982 omitted.

Top blot (A) : ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B) : As above, except ab6982 omitted.

All lanes:

Western blot - Anti-Adenovirus Type 5 antibody (ab6982)

false