Anti-Adenovirus Type 5 antibody is a rabbit polyclonal antibody that is used to detect Adenovirus Type 5 in ELISA, ICC/IF, Neut, Western blot. Suitable for Human adenovirus 5 samples.
- Cited in over 80 publications
- Tried and trusted by researchers since 2001
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- Nature biomedical engineering 7:928-9422023Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaibin Hao,Shipo Wu,Jiake Lin,Zitong Zheng,Yuemin Zhou,Ying Zhang,Qiang Guo,Fengchao Tian,Mengsu Zhao,Yi Chen,Xurong Xu,Lihua Hou,Xiaoyu Wang,Ruikang TangPubMed 36959404
- Journal of virology 97:e01539222023Fragile X-Related Protein FXR1 Controls Human Adenovirus Capsid mRNA Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZamaneh Hajikhezri,Yanina Kaira,Erik Schubert,Mahmoud Darweesh,Catharina Svensson,Göran Akusjärvi,Tanel PungaPubMed 36749074
- Journal for immunotherapy of cancer 10:2023Systemic cellular viroimmunotherapy for canine high-grade gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Cloquell,Isidro Mateo,Stefano Gambera,Martí Pumarola,Ramon Alemany,Javier García-Castro,Ana Judith Perisé-BarriosPubMed 36600663
- Journal of virology 97:e01467222022Molecular Mechanism of Adenovirus Late Protein L4-100K Chaperones the Trimerization of Hexon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Zhu,Xiang-Gang Xu,Tian-Xin Zhang,Xu-Pu Wang,Chun-He Zhang,Chun-Yu Wang,Chu Wang,Jia-Xin Wu,Bin Yu,Xiang-Hui YuPubMed 36475768
- Molecular therapy oncolytics 27:3-132022Oncolytic virus-mediated p53 overexpression promotes immunogenic cell death and efficacy of PD-1 blockade in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroyuki Araki,Hiroshi Tazawa,Nobuhiko Kanaya,Yoshinori Kajiwara,Motohiko Yamada,Masashi Hashimoto,Satoru Kikuchi,Shinji Kuroda,Ryuichi Yoshida,Yuzo Umeda,Yasuo Urata,Shunsuke Kagawa,Toshiyoshi FujiwaraPubMed 36212775
- Nature communications 13:32892022Low-dose self-amplifying mRNA COVID-19 vaccine drives strong protective immunity in non-human primates against SARS-CoV-2 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy R Rappaport,Sue-Jean Hong,Ciaran D Scallan,Leonid Gitlin,Arvin Akoopie,Gregory R Boucher,Milana Egorova,J Aaron Espinosa,Mario Fidanza,Melissa A Kachura,Annie Shen,Gloria Sivko,Anne Van Abbema,Robert L Veres,Karin JoossPubMed 35672369
- International journal of molecular sciences 23:2022Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelen Bergquist,Raviteja Inturi,Rula Zain,Tanel PungaPubMed 35328524
- Nature communications 13:11822022Self-attenuating adenovirus enables production of recombinant adeno-associated virus for high manufacturing yield without contamination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiheng Su,Maria I Patrício,Margaret R Duffy,Jakub M Krakowiak,Leonard W Seymour,Ryan CawoodPubMed 35256603
- Vaccines 10:2022E4orf1 Suppresses -Deleted Adenovirus Vaccine-Induced Immune Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKotou Sangare,Sabrina Helmold Hait,Madison Moore,Christopher Hogge,Tanya Hoang,Mohammad Arif Rahman,David J Venzon,Celia LaBranche,David Montefiori,Marjorie Robert-Guroff,Michael A ThomasPubMed 35214753
- Molecular therapy oncolytics 24:230-2482022Superior infectivity of the fiber chimeric oncolytic adenoviruses Ad5/35 and Ad5/3 over Ad5-delta-24-RGD in primary glioma cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksei A Stepanenko,Anastasiia O Sosnovtseva,Marat P Valikhov,Anastasia A Chernysheva,Sergey A Cherepanov,Gaukhar M Yusubalieva,Zsolt Ruzsics,Anastasiia V Lipatova,Vladimir P ChekhoninPubMed 35071746
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| WB | ICC/IF | ELISA | Neut | |
|---|---|---|---|---|
| Human adenovirus 5 | Tested | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human adenovirus 5 | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human adenovirus 5 | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human adenovirus 5 | Dilution info - | Notes Recognizes Ad5 immobilized on microtiter plates. |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human adenovirus 5 | Dilution info - | Notes This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137 |
Alternative names
AdV 5, Adenovirus 5
Recommended products
Anti-Adenovirus Type 5 antibody is a rabbit polyclonal antibody that is used to detect Adenovirus Type 5 in ELISA, ICC/IF, Neut, Western blot. Suitable for Human adenovirus 5 samples.
- Cited in over 80 publications
- Tried and trusted by researchers since 2001
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Adenovirus Type 5 antibody (ab6982) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, Neut, WB in human adenovirus 5 samples.
Anti-Adenovirus Type 5 antibody (ab6982) specifically detects Adenovirus Type 5 (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Adenovirus Type 5 antibody (ab6982) has high sensitivity and specificity.
Anti-Adenovirus Type 5 antibody (ab6982) has been cited over 85 times in peer reviewed journals and is trusted by the scientific community.
Anti-Adenovirus Type 5 antibody (ab6982) has 9 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Image courtesy of an anonymous customer reviewWestern blot - Anti-Adenovirus Type 5 antibody (ab6982)
Blocked with 5% milk
All lanes: Western blot - Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1: human hepatocytes whole cell lysate at 20 µg
Lane 2: human hepatocytes infected with Adv-iNOS whole cell lysate at 20 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 130 kDa
Exposure time: 20s
Image courtesy of an anonymous customer review.Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)
ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).
Bottom blot (B): As above, except ab6982 omitted.
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).
Bottom blot (B): As above, except ab6982 omitted.All lanes: Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
Image courtesy of an anonymous customer review.Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
Blocking step was performed using 5% milk for 1 hour at 23°C.
All lanes: Western blot - Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1: Whole cell lysate prepared from U2OS cells. at 35 µg
Lane 2: Whole cell lysate prepared from U2OS cells infected with Adenovirus 5. at 35 µg
SecondaryAll lanes: Goat anti-rabbit conjugated to IRDye 700DX
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