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Rabbit Recombinant Monoclonal CAPSH antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, I-ELISA and reacts with Transfected cell lysate - Human adenovirus 5, Transfected cell line - Human adenovirus 5, Synthetic peptide - Human adenovirus 5 samples.

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Images

Western blot - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (AB316852), expandable thumbnail
  • Western blot - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (AB316852), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (AB316852), expandable thumbnail
  • Immunoprecipitation - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (AB316852), expandable thumbnail
  • Indirect ELISA - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (AB316852), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IPI-ELISA
Human adenovirus 5
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Synthetic peptide - Human adenovirus 5
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell line - Human adenovirus 5
Not recommended
Tested
Tested
Tested
Not recommended
Not recommended
Transfected cell lysate - Human adenovirus 5
Tested
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human adenovirus 5
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Human adenovirus 5
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human adenovirus 5, Synthetic peptide - Human adenovirus 5
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human adenovirus 5
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Human adenovirus 5
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human adenovirus 5, Synthetic peptide - Human adenovirus 5
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human adenovirus 5
Dilution info
1/4000
Notes

-

Predicted
Predicted

Species
Human adenovirus 5
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human adenovirus 5, Synthetic peptide - Human adenovirus 5
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human adenovirus 5
Dilution info
1/5000
Notes

-

Predicted
Predicted

Species
Human adenovirus 5
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human adenovirus 5, Synthetic peptide - Human adenovirus 5
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human adenovirus 5
Dilution info
1/30
Notes

-

Predicted
Predicted

Species
Human adenovirus 5
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human adenovirus 5, Synthetic peptide - Human adenovirus 5
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human adenovirus 5
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Human adenovirus 5
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human adenovirus 5, Transfected cell line - Human adenovirus 5
Dilution info
-
Notes

-

Target data

Function

Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein. Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus.

Alternative names

Hexon protein, CP-H, Protein II, L3

Recommended products

Rabbit Recombinant Monoclonal CAPSH antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, I-ELISA and reacts with Transfected cell lysate - Human adenovirus 5, Transfected cell line - Human adenovirus 5, Synthetic peptide - Human adenovirus 5 samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28237-57
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Adenovirus Type 5 hexon also known as hexon protein or 5 protein is a major structural component of adenovirus capsid with a mass of approximately 960 kDa. Hexon proteins form the outer shell of the virus giving it stability and shape. These proteins are heavily expressed on the surface of human adenovirus serotype 5 a common virus strain used in therapeutic vector development. Hexon comprises three regions: the hypervariable regions conserved regions and the base that interacts with other capsid proteins.

Biological function summary

Adenovirus hexon proteins participate in the assembly and stability of the virus capsid complex. The hexon proteins interact with other components like penton base and fiber proteins to form a robust and protective viral coat. Their function involves binding to cell surface receptors such as the coxsackievirus and adenovirus receptor (CAR) allowing the virus to attach to and enter host cells. This interaction is key in viral infections and also in viral vector efficiency.

Pathways

The adenovirus Type 5 hexon protein plays important roles in immune evasion and viral entry pathways. The hexon protein helps the virus escape host immune detection by eluding neutralizing antibodies. The internal pathways also involve interactions with proteins like E1A which modulates host cell cycle pathways for viral replication. These interactions integrate hexon proteins into the broader adenoviral life cycle and infection processes.

Associated diseases and disorders

Infections involving adenovirus Type 5 relate to respiratory illnesses and conjunctivitis. Respiratory infections often occur in crowded environments such as schools and military barracks and the hexon protein's interaction with the immune system can exacerbate symptoms. Additionally due to its prevalent role in viral vector design research on hexon relates to gene therapy contexts scrutinizing how hexon modifications impact disease treatment and host immune response. Hemagglutinin might connect to respiratory disorders linked to adenovirus making it also relevant in developing therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Western blot - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852) at 1/1000 dilution

    Lane 1: 293T cells (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 293T cells transfected with a Adenovirus Type 5 Hexon expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 108 kDa, 104 kDa

    Exposure time: 81s

  • Western blot - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Western blot - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    This antibody does not cross-react with Adenovirus Type 2 Hexon and Adenovirus Type 4 Hexon.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852) at 1/1000 dilution

    Lane 1: His-tagged Adenovirus Type 2 Hexon protein at 10 ng with 5% NFDM/TBST

    Lane 2: His-tagged Adenovirus Type 5 Hexon protein at 10 ng with 5% NFDM/TBST

    Lane 3: His-tagged Adenovirus Type 4 Hexon protein at 10 ng with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 36 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with HAdV-5 Hexon vector containing a myc-His-tag® (Middle) / 293T cells transfected with an epmty vector containing a myc-His-tag® (Right) cells labelling Adenovirus Type 5 Hexon with ab316852 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Cells were stained with anti-myc tag conjugated to Alexa Fluor®647.

  • Immunoprecipitation - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Immunoprecipitation - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    Adenovirus Type 5 Hexon was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a Adenovirus Type 5 Hexon expression vector containing a myc-His-tag®, whole cell lysate with ab316852 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316852 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a Adenovirus Type 5 Hexon expression vector containing a myc-His-tag®, whole cell lysate

    Lane 2: ab316852 IP in 293T (human embryonic kidney epithelial cell) cells transfected with a Adenovirus Type 5 Hexon expression vector containing a myc-His-tag®, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316852 in 293T cells transfected with a Adenovirus Type 5 Hexon expression vector containing a myc-His-tag®, whole cell lysate

    All lanes: Immunoprecipitation - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852) at 1/30 dilution

    All lanes: 293T (human embryonic kidney epithelial cell) cells transfected with a Adenovirus Type 5 Hexon expression vector containing a myc-His-tag®, whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 10s

  • Indirect ELISA - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Indirect ELISA - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    Indirect ELISA analysis of ab316852 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

    Antigen: Adenovirus Type 5 Hexon.

    Antigen concentration: 1000 ng/ml

  • Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an Adenovirus Type 5 Hexon vector containing a myc-His-tag®. cells labelling Adenovirus Type 5 Hexon with ab316852 at 1/4000 (0.125 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing positive staining in 293T cells transfected with an Adenovirus Type 5 Hexon vector containing a myc-His-tag®.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Adenovirus Type 5 Hexon with ab316852 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    No staining on rat pancreas.

    The section was incubated with ab316852 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Adenovirus Type 5 Hexon with ab316852 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    No staining on mouse pancreas.

    The section was incubated with ab316852 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Adenovirus Type 5 Hexon with ab316852 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    No staining on human pancreas.

    The section was incubated with ab316852 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenovirus Type 5 Hexon antibody [EPR28237-57] (ab316852)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Adenovirus Type 5 Hexon expression vector containing a myc-His-tag®. (B) HEK-293T transfected with empty vector containing a myc-His-tag®. tissue labeling Adenovirus Type 5 Hexon with ab316852 at 1/500 (0.998 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) HEK-293T transfected with a Adenovirus Type 5 Hexon vector containing a myc-His-tag®. No staining on (B) HEK-293T transfected with empty vector containing a myc-His-tag®.

    The section was incubated with ab316852 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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