Anti-ADGRE5 antibody [HL1924] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (AB325845)

ab325845 detects CD97 protein at cell membrane and cytoplasmic vesicles by immunofluorescent analysis. Sample : U937 cells were fixed in ice-cold MeOH for 5 min. Green : CD97 stained by ab325845 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (AB325845)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325845 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (ab325845) at 1/500 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (AB325845)

Boiled and unboiled wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325845 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (ab325845) at 1/1000 dilution

Lane 1:

Boiled wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

Boiled ADGRE5 knockout (KO) HeLa cell extracts at 30 µg

Lane 3:

Unboiled wild-type (WT) HeLa cell extracts at 30 µg

Lane 4:

Unboiled ADGRE5 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (AB325845)

ab325845 detects CD97 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in MeOH. Green : CD97 stained by ab325845 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (AB325845)

Unboiled non-transfected (–) and transfected (+) Expi293 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325845 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (ab325845) at 1/5000 dilution

Lanes 1, 3, 5 and 7:

Unboiled non-transfected (–) Expi293 whole cell extracts at 30 µg

Lane 2:

Unboiled ADGRE1 transfected (+) Expi293 whole cell extracts at 30 µg

Lane 4:

Unboiled ADGRE5 transfected (+) Expi293 whole cell extracts at 30 µg

Lane 6:

Unboiled ADGRG5 transfected (+) Expi293 whole cell extracts at 30 µg

Lane 8:

Unboiled ADGRL1 transfected (+) Expi293 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (AB325845)

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325845 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (ab325845) at 1/1000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged ADGRE5 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged ADGRE5 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (AB325845)

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325845 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1924] - BSA and Azide free (ab325845) at 1/1000 dilution

All lanes:

MDCK whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true