Anti-ADGRE5 antibody [HL1925] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (AB325846)

Immunoprecipitation of CD97 protein from U937 using 5 μg of ab325846. Western blot analysis was performed using ab325846. Secondary : HRP-conjugated anti rabbit IgG antibody.

All lanes:

Immunoprecipitation - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (ab325846) at 5 µg

Lane 1:

Input

Lane 3:

Immunoprecipitation of CD97 protein from U937

Secondary

All lanes:

HRP-conjugated anti rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (AB325846)

Wild-type (WT) and ADGRE5 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325846 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (ab325846) at 1/500 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

ADGRE5 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (AB325846)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325846 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (ab325846) at 1/500 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (AB325846)

U937 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with ab325846 diluted at 1 : 1000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (ab325846) at 1/1000 dilution

Lane 1:

U937 whole cell extracts at 30 µg

Lane 2:

U937 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (AB325846)

Wild-type (WT) and CD97 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with ab325846 diluted at 1 : 1000 and the highly cited competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (ab325846) at 1/1000 dilution

Lane 1:

Boiled Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

Boiled CD97 knockout (KO) HeLa cell extracts at 30 µg

Lane 3:

Unboiled Wild-type (WT) HeLa cell extracts at 30 µg

Lane 4:

Unboiled CD97 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (AB325846)

Untreated (–) and treated (+) U937 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325846 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (ab325846) at 1/2000 dilution

Lane 1:

Untreated (–) U937 whole cell extracts at 30 µg

Lane 2:

24 ug/mL Tunicamycin, 24hr treated (+) U937 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (AB325846)

Boiled and unboiled U937 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325846 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (ab325846) at 1/2000 dilution

Lane 1:

Boiled U937 whole cell extracts at 30 µg

Lane 2:

Boiled U937 membrane extracts at 30 µg

Lane 3:

Unboiled U937 whole cell extracts at 30 µg

Lane 4:

Unboiled U937 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (AB325846)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325846 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ADGRE5 antibody [HL1925] - BSA and Azide free (ab325846) at 1/2000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged ADGRE5 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false