AB137077

Anti-ADH4 antibody [EPR9483]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal ADH4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

ADH2, ADH4, All-trans-retinol dehydrogenase [NAD(+)] ADH4, Alcohol dehydrogenase 2, Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-ADH4 antibody [EPR9483] (AB137077)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ADH4 antibody [EPR9483] (AB137077)

Immunofluorescent analysis of HepG2 cells labelling ADH4 with ab137077 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH4 antibody [EPR9483] (AB137077)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH4 antibody [EPR9483] (AB137077)

Immunohistochemical analysis of paraffin-embedded Human liver tissue carcinoma labelling ADH4 with ab137077 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ADH4 antibody [EPR9483] (AB137077)

All lanes:

Western blot - Anti-ADH4 antibody [EPR9483] (ab137077) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

false

Carrier free

Anti-ADH4 antibody [EPR9483] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9483

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADH4 also known as alcohol dehydrogenase 4 is an enzyme with a mass of approximately 40 kDa. This enzyme plays a role in the oxidation of alcohols within the digestive system. Not just limited to the digestive system ADH4 also shows expression in the liver and kidneys where it contributes to the metabolic processes. Its action involves converting ethanol and other alcohols to their corresponding aldehydes which are then further metabolized.

Biological function summary

The enzyme participates in the oxidation of retinol to retinoic acid a process essential for diverse biological functions. While ADH4 operates primarily as a standalone enzyme it still interacts with other members of the alcohol dehydrogenase family within the liver. These interactions facilitate intermediary metabolism and help maintain homeostasis. The enzyme's role extends beyond simple alcohol metabolism implicating it in broader metabolic contexts.

Pathways

ADH4 occupies a definite position in the ethanol metabolism pathway. It cooperates with enzymes like aldehyde dehydrogenase to process ethanol into acetic acid a step important for energy production and detoxification. Additionally its activity relates to the retinoic acid signaling pathway. Through this pathway it interacts indirectly with enzymes that process retinoids connecting it to cellular differentiation and vision.

ADH4 has associations with alcohol-related liver disease and fetal alcohol syndrome. In liver disease ADH4's excessive ethanol oxidation can lead to toxic aldehyde accumulation contributing to tissue damage. Its interaction with aldehyde dehydrogenase becomes significant as any dysfunction in this relation might exacerbate disease severity. Moreover disruptions in retinoic acid metabolism where ADH4 plays a part can link to disorders affecting embryonic development highlighting its role in conditions like fetal alcohol syndrome.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol (PubMed : 17279314). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed : 16081420). Also catalyzes the reduction of benzoquinones (PubMed : 10514444).

See full target information ADH4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:251 PubMed40410177

2025

Absence of Rnf126 causes male infertility with multiple morphological abnormalities of the sperm flagella.

Applications

Unspecified application

Species

Unspecified reactive species

Shengnan Wang,Zihan Qin,Juan Liu,Jie Liu,Qiaohua Xiong,Zexiao Wei,Li Wang,Yuming Cao

Clinical and translational medicine 15:e70198 PubMed39834100

2025

Single-cell landscape of the intrahepatic ecosystem in alcohol-related liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Zhao,Senyan Wang,Qi Liu,Wenjuan Wei,Xiaoyan Sun,Hao Song,Jing Xu,Shuijun Zhang,Hongyang Wang,Jing Fu

Cancer medicine 13:e70284 PubMed39540710

2024

Predictive Prognostic Model for Hepatocellular Carcinoma Based on Seven Genes Participating in Arachidonic Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Gu,Jing Wang,Jun Guan,Guojun Li,Xiao Ma,Yanli Ren,Shanshan Wu,Chao Chen,Haihong Zhu

Oncology letters 25:3 PubMed36419756

2022

Alcohol dehydrogenase 4 is a TP53-associated gene signature for the prediction of prognosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Hui-Hong Jiang,Zhen-Yu Wang,Bo Zhai,Mou-Bin Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADH4 antibody [EPR9483]

Immunocytochemistry/ Immunofluorescence - Anti-ADH4 antibody [EPR9483] (AB137077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH4 antibody [EPR9483] (AB137077)

Western blot - Anti-ADH4 antibody [EPR9483] (AB137077)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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