Rabbit Recombinant Monoclonal ADH4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (AB248803), expandable thumbnail](https://content.abcam.com/products/images/adh4-antibody-epr9483-bsa-and-azide-free-ab248803--western-blot-img81808.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (AB248803)")
![Immunocytochemistry/ Immunofluorescence - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (AB248803), expandable thumbnail](https://content.abcam.com/products/images/adh4-antibody-epr9483-bsa-and-azide-free-ab248803--immunocytochemistry-immunofluorescence-img118684.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (AB248803)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (AB248803), expandable thumbnail](https://content.abcam.com/products/images/adh4-antibody-epr9483-bsa-and-azide-free-ab248803--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98352.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (AB248803)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol (PubMed:17279314). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed:16081420). Also catalyzes the reduction of benzoquinones (PubMed:10514444).
Alternative names
ADH2, ADH4, All-trans-retinol dehydrogenase [NAD(+)] ADH4, Alcohol dehydrogenase 2, Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain
Recommended products
Rabbit Recombinant Monoclonal ADH4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9483
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248803 is the carrier-free version of Anti-ADH4 antibody [EPR9483] ab137077.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADH4 also known as alcohol dehydrogenase 4 is an enzyme with a mass of approximately 40 kDa. This enzyme plays a role in the oxidation of alcohols within the digestive system. Not just limited to the digestive system ADH4 also shows expression in the liver and kidneys where it contributes to the metabolic processes. Its action involves converting ethanol and other alcohols to their corresponding aldehydes which are then further metabolized.
- Biological function summary
The enzyme participates in the oxidation of retinol to retinoic acid a process essential for diverse biological functions. While ADH4 operates primarily as a standalone enzyme it still interacts with other members of the alcohol dehydrogenase family within the liver. These interactions facilitate intermediary metabolism and help maintain homeostasis. The enzyme's role extends beyond simple alcohol metabolism implicating it in broader metabolic contexts.
- Pathways
ADH4 occupies a definite position in the ethanol metabolism pathway. It cooperates with enzymes like aldehyde dehydrogenase to process ethanol into acetic acid a step important for energy production and detoxification. Additionally its activity relates to the retinoic acid signaling pathway. Through this pathway it interacts indirectly with enzymes that process retinoids connecting it to cellular differentiation and vision.
- Associated diseases and disorders
ADH4 has associations with alcohol-related liver disease and fetal alcohol syndrome. In liver disease ADH4's excessive ethanol oxidation can lead to toxic aldehyde accumulation contributing to tissue damage. Its interaction with aldehyde dehydrogenase becomes significant as any dysfunction in this relation might exacerbate disease severity. Moreover disruptions in retinoic acid metabolism where ADH4 plays a part can link to disorders affecting embryonic development highlighting its role in conditions like fetal alcohol syndrome.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803), expandable thumbnail](https://content.abcam.com/products/images/adh4-antibody-epr9483-bsa-and-azide-free-ab248803--western-blot-img81808.jpg "Western blot - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803)")
Western blot - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803)
This data was developed using Anti-ADH4 antibody [EPR9483] ab137077, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ADH4 antibody [EPR9483] (Anti-ADH4 antibody [EPR9483] ab137077) at 1/1000 dilution
All lanes: Human fetal liver tissue lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803), expandable thumbnail](https://content.abcam.com/products/images/adh4-antibody-epr9483-bsa-and-azide-free-ab248803--immunocytochemistry-immunofluorescence-img118684.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803)")
Immunocytochemistry/ Immunofluorescence - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803)
This data was developed using Anti-ADH4 antibody [EPR9483] ab137077, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labelling ADH4 with Anti-ADH4 antibody [EPR9483] ab137077 at 1/250 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803), expandable thumbnail](https://content.abcam.com/products/images/adh4-antibody-epr9483-bsa-and-azide-free-ab248803--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98352.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH4 antibody [EPR9483] - BSA and Azide free (ab248803)
This data was developed using Anti-ADH4 antibody [EPR9483] ab137077, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue carcinoma labelling ADH4 with Anti-ADH4 antibody [EPR9483] ab137077 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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