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Rabbit Recombinant Monoclonal ADH5 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.


Images

Western blot - Anti-ADH5 antibody [EPR12885(B)] (AB177932), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (AB177932), expandable thumbnail
  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (AB177932), expandable thumbnail
  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (AB177932), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (AB177932), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Expected
Not recommended
Not recommended
Tested
Not recommended
Rat
Expected
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/8000
Notes

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

-

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione (PubMed:8460164). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed:16081420). Class-III ADH is remarkably ineffective in oxidizing ethanol (PubMed:8460164). Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage (PubMed:33355142). Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation (By similarity).

Alternative names

ADHX, FDH, ADH5, Alcohol dehydrogenase class-3, Alcohol dehydrogenase 5, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class-III, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase, FALDH, GSH-FDH

Recommended products

Rabbit Recombinant Monoclonal ADH5 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12885(B)
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ADH5 also known as alcohol dehydrogenase class-3 or glutathione-dependent formaldehyde dehydrogenase is an enzyme with a mass of approximately 42 kDa. It plays a role in alcohol metabolism specifically in the detoxification of formaldehyde. The protein is expressed in many tissues including the liver kidney and lung. ADH5 belongs to the alcohol dehydrogenase protein family and distinguishes itself by its ability to metabolize both aliphatic and aromatic compounds.

Biological function summary

Alcohol metabolism involves more than one enzyme and ADH5 functions within a network of metabolic reactions. This enzyme contributes to formaldehyde detoxification where it oxidizes formaldehyde to formic acid. The ADH5 enzyme operates in the cytosol and takes part in the catabolism of harmful aldehydes. Its action supports cellular protection reducing toxicity from chemical exposure and ensuring normal cellular metabolism.

Pathways

ADH5 plays significant roles in methanol and formaldehyde metabolic pathways. It acts alongside enzymes like alcohol dehydrogenase 1 (ADH1) and aldehyde dehydrogenase (ALDH) in processing formaldehyde into less toxic substances. In the methanol metabolism pathway interactions with ADH1 highlight its role in ethanol to acetaldehyde conversion. The pathway interactions emphasize its importance in reducing oxidative stress and preventing damage from reactive aldehydes.

Associated diseases and disorders

ADH5's function aligns with conditions related to alcohol metabolism like formaldehyde sensitivity and methanol poisoning. Abnormal ADH5 activity can contribute to such conditions implicating oxidative stress responses. Associations also exist with other diseases related to aldehyde toxicity such as alcohol-related liver damage. Alongside ADH1 and ALDH ADH5 aids in maintaining homeostasis in cellular environments and disruptions can lead to pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932), expandable thumbnail

    Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

    All lanes: Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution

    All lanes: Human liver lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling ADH5 with purified ab177932 at 1/8000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932), expandable thumbnail

    Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

    ADH5 is highly expressed in liver, kidney and low expressed in brain as is described in PMID: 26412304

    All lanes: Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/5000 dilution

    Lane 1: Mouse liver lysates at 15 µg

    Lane 2: Mouse brain lysates at 15 µg

    Lane 3: Rat liver lysates at 15 µg

    Lane 4: Rat brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932), expandable thumbnail

    Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

    All lanes: Western blot - Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/1000 dilution

    Lane 1: Human fetal liver lysates at 10 µg

    Lane 2: HepG2 lysates at 10 µg

    Lane 3: Human fetal kidney lysates at 10 µg

    Lane 4: SH-SY5Y lysates at 10 µg

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] (ab177932)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with unpurified ab177932 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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