Rabbit Recombinant Monoclonal ADH5 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] - BSA and Azide free (AB250008), expandable thumbnail](https://content.abcam.com/products/images/adh5-antibody-epr12885b-bsa-and-azide-free-ab250008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104094.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] - BSA and Azide free (AB250008)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected | Not recommended |
| Mouse | Predicted | Not recommended | Not recommended | Expected | Not recommended |
| Rat | Predicted | Not recommended | Not recommended | Expected | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione (PubMed:8460164). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed:16081420). Class-III ADH is remarkably ineffective in oxidizing ethanol (PubMed:8460164).
Alternative names
Alcohol dehydrogenase class-3, Alcohol dehydrogenase 5, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class-III, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase, FALDH, FDH, GSH-FDH, ADHX, ADH5, FDH
Recommended products
Rabbit Recombinant Monoclonal ADH5 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Alternative names
Alcohol dehydrogenase class-3, Alcohol dehydrogenase 5, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class-III, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase, FALDH, FDH, GSH-FDH, ADHX, ADH5, FDH
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR12885(B)
- Purification technique
Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250008 is the carrier-free version of Anti-ADH5 antibody [EPR12885(B)] ab177932.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADH5 also known as alcohol dehydrogenase class-3 or glutathione-dependent formaldehyde dehydrogenase is an enzyme with a mass of approximately 42 kDa. It plays a role in alcohol metabolism specifically in the detoxification of formaldehyde. The protein is expressed in many tissues including the liver kidney and lung. ADH5 belongs to the alcohol dehydrogenase protein family and distinguishes itself by its ability to metabolize both aliphatic and aromatic compounds.
- Biological function summary
Alcohol metabolism involves more than one enzyme and ADH5 functions within a network of metabolic reactions. This enzyme contributes to formaldehyde detoxification where it oxidizes formaldehyde to formic acid. The ADH5 enzyme operates in the cytosol and takes part in the catabolism of harmful aldehydes. Its action supports cellular protection reducing toxicity from chemical exposure and ensuring normal cellular metabolism.
- Pathways
ADH5 plays significant roles in methanol and formaldehyde metabolic pathways. It acts alongside enzymes like alcohol dehydrogenase 1 (ADH1) and aldehyde dehydrogenase (ALDH) in processing formaldehyde into less toxic substances. In the methanol metabolism pathway interactions with ADH1 highlight its role in ethanol to acetaldehyde conversion. The pathway interactions emphasize its importance in reducing oxidative stress and preventing damage from reactive aldehydes.
- Associated diseases and disorders
ADH5's function aligns with conditions related to alcohol metabolism like formaldehyde sensitivity and methanol poisoning. Abnormal ADH5 activity can contribute to such conditions implicating oxidative stress responses. Associations also exist with other diseases related to aldehyde toxicity such as alcohol-related liver damage. Alongside ADH1 and ALDH ADH5 aids in maintaining homeostasis in cellular environments and disruptions can lead to pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] - BSA and Azide free (ab250008), expandable thumbnail](https://content.abcam.com/products/images/adh5-antibody-epr12885b-bsa-and-azide-free-ab250008--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104094.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] - BSA and Azide free (ab250008)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADH5 antibody [EPR12885(B)] - BSA and Azide free (ab250008)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling ADH5 with purified Anti-ADH5 antibody [EPR12885(B)] ab177932 at 1/8000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ADH5 antibody [EPR12885(B)] ab177932)
Downloads
Product protocols
- Visit the general protocols
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