AB186408

Anti-ADH7 antibody [EPR13949]

Name: Anti-ADH7 antibody [EPR13949] (ab186408) | Abcam
Brand: Abcam Limited
SKU: AB186408
Price: 345 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal ADH7 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

All-trans-retinol dehydrogenase [NAD(+)] ADH7, Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase, Omega-hydroxydecanoate dehydrogenase ADH7, Retinol dehydrogenase, ADH7

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] (AB186408)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] (AB186408)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ADH7 with ab186408 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Supplier Data

Western blot - Anti-ADH7 antibody [EPR13949] (AB186408)

All lanes:

Western blot - Anti-ADH7 antibody [EPR13949] (ab186408) at 1/1000 dilution

Lane 1:

Human stomach lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Carrier free

Anti-ADH7 antibody [EPR13949] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13949

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target ADH7 also known as alcohol dehydrogenase 7 or class IV ADH is an enzyme with a molecular mass of approximately 38 kDa. ADH7 belongs to the alcohol dehydrogenase family and participates in the oxidative reduction of alcohols to aldehydes or ketones. This enzyme is expressed mainly in the stomach and to a lesser extent in the liver and other tissues. The expression levels depend on genetic variation among different populations.

Biological function summary

ADH7 plays a role in metabolizing retinoids and ethanol in the body. It does not form a complex but acts as a monomer in enzymatic reactions. By converting alcohols to their aldehyde forms ADH7 participates in detoxification processes and helps maintain homeostasis of retinoid metabolites. This function involves the interaction of ADH7 with other alcohol dehydrogenases that process overlapping substrate types.

Pathways

ADH7 is involved in the retinol metabolism pathway and ethanol degradation pathway. In the retinol metabolism pathway it works alongside other enzymes such as retinol dehydrogenase to maintain vitamin A homeostasis. These pathways are important for detoxifying alcohols and maintaining cell signaling processes. Through some metabolic connections ADH7 interacts with proteins like CYP2E1 in ethanol degradation highlighting a collaborative yet distinct pathway environment.

ADH7 shows a connection to alcoholism and gastric cancer. Genetic variations in ADH7 may influence individual susceptibility to alcohol dependence due to altered alcohol metabolism. Additionally owing to its presence in gastric tissues ADH7's interaction with aldehyde dehydrogenase can modulate the development of gastric cancer under certain conditions emphasizing its relevance in disease etiology and potential therapeutic exploration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, and omega-hydroxy fatty acids and their derivatives (PubMed : 15369820, PubMed : 16787387, PubMed : 9600267). Oxidizes preferentially all trans-retinol, all-trans-4-hydroxyretinol, 9-cis-retinol, 2-hexenol, and long chain omega-hydroxy fatty acids such as juniperic acid (PubMed : 15369820, PubMed : 16787387, PubMed : 9600267). In vitro can also catalyze the NADH-dependent reduction of all-trans-retinal and aldehydes and their derivatives (PubMed : 15369820, PubMed : 16787387, PubMed : 9600267). Reduces preferentially all trans-retinal, all-trans-4-oxoretinal and hexanal (PubMed : 15369820, PubMed : 16787387). Catalyzes in the oxidative direction with higher efficiency (PubMed : 15369820, PubMed : 16787387). Therefore may participate in retinoid metabolism, fatty acid omega-oxidation, and elimination of cytotoxic aldehydes produced by lipid peroxidation (PubMed : 15369820, PubMed : 16787387, PubMed : 9600267).

See full target information ADH7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 17:2463581 PubMed39924917

2025

infection induces DNA double-strand breaks through the ACVR1/IRF3/POLD1 signaling axis to drive gastric tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinbo Xu,Xiao Fei,Huan Wang,Xidong Wu,Yuan Zhan,Xin Li,Yan'an Zhou,Chunxi Shu,Cong He,Yi Hu,Jianping Liu,Nonghua Lv,Nianshuang Li,Yin Zhu

iScience 26:108564 PubMed38213791

2024

Ethanol induces replication fork stalling and membrane stress in immortalized laryngeal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lore Hoes,Karin Voordeckers,Rüveyda Dok,Bram Boeckx,Bart Steemans,Diyavarshini Gopaul,Philippe Pasero,Sander K Govers,Diether Lambrechts,Sandra Nuyts,Kevin J Verstrepen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADH7 antibody [EPR13949]

Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] (AB186408)

Western blot - Anti-ADH7 antibody [EPR13949] (AB186408)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise