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AB250872

Anti-ADH7 antibody [EPR13949] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ADH7 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

All-trans-retinol dehydrogenase [NAD(+)] ADH7, Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase, Omega-hydroxydecanoate dehydrogenase ADH7, Retinol dehydrogenase, ADH7

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (AB250872)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (AB250872)

This data was developed using ab186408, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ADH7 with ab186408 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Western blot - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (AB250872)
  • WB

Supplier Data

Western blot - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (AB250872)

This data was developed using ab186408, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ADH7 antibody [EPR13949] (<a href='/en-us/products/primary-antibodies/adh7-antibody-epr13949-ab186408'>ab186408</a>) at 1/1000 dilution

Lane 1:

Human stomach lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13949

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250872 is the carrier-free version of ab186408.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target ADH7 also known as alcohol dehydrogenase 7 or class IV ADH is an enzyme with a molecular mass of approximately 38 kDa. ADH7 belongs to the alcohol dehydrogenase family and participates in the oxidative reduction of alcohols to aldehydes or ketones. This enzyme is expressed mainly in the stomach and to a lesser extent in the liver and other tissues. The expression levels depend on genetic variation among different populations.
Biological function summary

ADH7 plays a role in metabolizing retinoids and ethanol in the body. It does not form a complex but acts as a monomer in enzymatic reactions. By converting alcohols to their aldehyde forms ADH7 participates in detoxification processes and helps maintain homeostasis of retinoid metabolites. This function involves the interaction of ADH7 with other alcohol dehydrogenases that process overlapping substrate types.

Pathways

ADH7 is involved in the retinol metabolism pathway and ethanol degradation pathway. In the retinol metabolism pathway it works alongside other enzymes such as retinol dehydrogenase to maintain vitamin A homeostasis. These pathways are important for detoxifying alcohols and maintaining cell signaling processes. Through some metabolic connections ADH7 interacts with proteins like CYP2E1 in ethanol degradation highlighting a collaborative yet distinct pathway environment.

ADH7 shows a connection to alcoholism and gastric cancer. Genetic variations in ADH7 may influence individual susceptibility to alcohol dependence due to altered alcohol metabolism. Additionally owing to its presence in gastric tissues ADH7's interaction with aldehyde dehydrogenase can modulate the development of gastric cancer under certain conditions emphasizing its relevance in disease etiology and potential therapeutic exploration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, and omega-hydroxy fatty acids and their derivatives (PubMed : 15369820, PubMed : 16787387, PubMed : 9600267). Oxidizes preferentially all trans-retinol, all-trans-4-hydroxyretinol, 9-cis-retinol, 2-hexenol, and long chain omega-hydroxy fatty acids such as juniperic acid (PubMed : 15369820, PubMed : 16787387, PubMed : 9600267). In vitro can also catalyze the NADH-dependent reduction of all-trans-retinal and aldehydes and their derivatives (PubMed : 15369820, PubMed : 16787387, PubMed : 9600267). Reduces preferentially all trans-retinal, all-trans-4-oxoretinal and hexanal (PubMed : 15369820, PubMed : 16787387). Catalyzes in the oxidative direction with higher efficiency (PubMed : 15369820, PubMed : 16787387). Therefore may participate in retinoid metabolism, fatty acid omega-oxidation, and elimination of cytotoxic aldehydes produced by lipid peroxidation (PubMed : 15369820, PubMed : 16787387, PubMed : 9600267).
See full target information ADH7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

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