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AB229146

Anti-ADHFE1 antibody

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(1 Publication)

Rabbit Polyclonal ADHFE1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADHFE1.

View Alternative Names

HMFT2263, ADHFE1, HOT, Alcohol dehydrogenase iron-containing protein 1, Fe-containing alcohol dehydrogenase, ADHFe1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ADHFE1 antibody (AB229146)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADHFE1 antibody (AB229146)

2% paraformaldehyde/culture medium-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ADHFE1 (green) using ab229146 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADHFE1 antibody (AB229146)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADHFE1 antibody (AB229146)

Paraffin-embedded human hepatoma tissue stained for ADHFE1 using ab229146 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-ADHFE1 antibody (AB229146)
  • WB

Supplier Data

Western blot - Anti-ADHFE1 antibody (AB229146)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-ADHFE1 antibody (ab229146) at 1/1000 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ADHFE1. The exact immunogen used to generate this antibody is proprietary information.

Q8IWW8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADHFE1 also known as alcohol dehydrogenase iron-containing 1 is a mitochondrial protein with a molecular mass of approximately 62 kDa. It is expressed in various tissues with higher levels in the liver kidney and heart. Functioning as a member of the iron-containing alcohol dehydrogenase family ADHFE1 catalyzes the conversion of alcohols into aldehydes an essential step in the metabolic pathway involving alcohols.
Biological function summary

The enzyme facilitates important redox reactions that contribute to various metabolic processes. ADHFE1 is not part of a larger complex but acts independently in its role of oxidizing alcohols to aldehydes. It impacts metabolic regulation by modulating the levels of substrates and products involved in energy production and detoxification which is significant for maintaining cellular homeostasis.

Pathways

ADHFE1 plays an important role in the metabolic network of energy conversion and alcohol detoxification. It is integrated into the glycolytic pathway and interacts with other proteins like G6PD and LDHA which function in glucose metabolism and lactate production respectively. These interactions support cellular energy production and manage the fate of glucose-derived metabolites.

ADHFE1 involvement relates to metabolic disorders such as alcohol dependence and non-alcoholic fatty liver disease (NAFLD). In alcohol dependence ADHFE1's activity influences the concentration of acetaldehyde which may contribute to the disease's pathology. Additionally its expression and interaction with proteins like ADH1C and ALDH2 are relevant in the context of liver diseases impacting the metabolic balance and detoxification processes in the liver.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.
See full target information ADHFE1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 18:4421-4428 PubMed31611951

2019

STAMBPL1 knockdown has antitumour effects on gastric cancer biological activities.

Applications

Unspecified application

Species

Unspecified reactive species

Da-Jun Yu,Jun Qian,Xin Jin,Jing Li,Chen-Xu Guo,Xi-Cheng Yue
View all publications
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Product promise

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