JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249768

Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ADHFE1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

HMFT2263, ADHFE1, HOT, Alcohol dehydrogenase iron-containing protein 1, Fe-containing alcohol dehydrogenase, ADHFe1

2 Images
Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (AB249768)
  • IP

Supplier Data

Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (AB249768)

This data was developed using ab173530, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver lysate or (Lane 2) 1X PBS (negative control) using ab173530 at a 1/10 dilution to immunoprecipitate ADHFE1, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] (<a href='/en-us/products/primary-antibodies/adhfe1-antibody-epr12501-ab173530'>ab173530</a>)

Predicted band size: 50 kDa

false

Western blot - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (AB249768)
  • WB

Supplier Data

Western blot - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (AB249768)

This data was developed using ab173530, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ADHFE1 antibody [EPR12501] (<a href='/en-us/products/primary-antibodies/adhfe1-antibody-epr12501-ab173530'>ab173530</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysates at 10 µg

Lane 2:

Human fetal kidney lysates at 10 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12501

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Product details

ab249768 is the carrier-free version of ab173530.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADHFE1 also known as alcohol dehydrogenase iron-containing 1 is a mitochondrial protein with a molecular mass of approximately 62 kDa. It is expressed in various tissues with higher levels in the liver kidney and heart. Functioning as a member of the iron-containing alcohol dehydrogenase family ADHFE1 catalyzes the conversion of alcohols into aldehydes an essential step in the metabolic pathway involving alcohols.
Biological function summary

The enzyme facilitates important redox reactions that contribute to various metabolic processes. ADHFE1 is not part of a larger complex but acts independently in its role of oxidizing alcohols to aldehydes. It impacts metabolic regulation by modulating the levels of substrates and products involved in energy production and detoxification which is significant for maintaining cellular homeostasis.

Pathways

ADHFE1 plays an important role in the metabolic network of energy conversion and alcohol detoxification. It is integrated into the glycolytic pathway and interacts with other proteins like G6PD and LDHA which function in glucose metabolism and lactate production respectively. These interactions support cellular energy production and manage the fate of glucose-derived metabolites.

ADHFE1 involvement relates to metabolic disorders such as alcohol dependence and non-alcoholic fatty liver disease (NAFLD). In alcohol dependence ADHFE1's activity influences the concentration of acetaldehyde which may contribute to the disease's pathology. Additionally its expression and interaction with proteins like ADH1C and ALDH2 are relevant in the context of liver diseases impacting the metabolic balance and detoxification processes in the liver.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.
See full target information ADHFE1
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com