AB62551

Anti-Adiponectin antibody

Name: Anti-Adiponectin antibody (ab62551) | Abcam
Brand: Abcam Limited
SKU: AB62551
Price: 670 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(16 Publications)

Rabbit Polyclonal Adiponectin antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human ADIPOQ.

View Alternative Names

ACDC, ACRP30, APM1, GBP28, ADIPOQ, Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, apM-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody (AB62551)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody (AB62551)

ab62551, at 1μg/ml, staining Adiponectin in rat brain tissue by immunohistochemistry using paraffin-embedded tissue.

Unknown

Western blot - Anti-Adiponectin antibody (AB62551)

Lane 1:

Western blot - Anti-Adiponectin antibody (ab62551) at 1 µg/mL

Lane 2:

Western blot - Anti-Adiponectin antibody (ab62551) at 2 µg/mL

Lane 3:

Western blot - Anti-Adiponectin antibody (ab62551) at 4 µg/mL

All lanes:

Rat brain cell lysate at 15 µg

Predicted band size: 26 kDa

Observed band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ADIPOQ. The exact immunogen used to generate this antibody is proprietary information.

Q15848

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.

Biological function summary

Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.

Pathways

Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.

Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

See full target information ADIPOQ

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes investigation 13:947-954 PubMed35023319

2022

Hyperlipidemic plasma molecules bind and inhibit adiponectin activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Qing Zhang,Sen Fan,Wen-Qing Wang,Wayne Bond Lau,Jian-Li Dai,Hai-Feng Zhang,Xiao-Ming Wang,Xiao-Gang Liu,Rong Li

Frontiers in endocrinology 11:592818 PubMed33424769

2020

Diphyllin Improves High-Fat Diet-Induced Obesity in Mice Through Brown and Beige Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Nan Duan,Xiang Ge,Hao-Wen Jiang,Hong-Jie Zhang,Yu Zhao,Jin-Long Li,Wei Zhang,Jing-Ya Li

Stem cell research & therapy 11:444 PubMed33076979

2020

DNMT1-mediated methylation inhibits microRNA-214-3p and promotes hair follicle stem cell differentiate into adipogenic lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Fangcao Jin,Min Li,Xuyang Li,Yunpeng Zheng,Kun Zhang,Xiaojun Liu,Bingjie Cai,Guangwen Yin

Investigative ophthalmology & visual science 61:12 PubMed32396633

2020

Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3.

Applications

Unspecified application

Species

Unspecified reactive species

Aysegül Tura,Christiane Thieme,Anton Brosig,Hartmut Merz,Mahdy Ranjbar,Siranush Vardanyan,Huaxin Zuo,Tjorge Maassen,Vinodh Kakkassery,Salvatore Grisanti

International journal of molecular sciences 21: PubMed32408716

2020

Effects of Maternal Resveratrol on Maternal High-Fat Diet/Obesity with or without Postnatal High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Hsin Hsu,Jiunn-Ming Sheen,I-Chun Lin,Hong-Ren Yu,Mao-Meng Tiao,You-Lin Tain,Li-Tung Huang

Journal of cardiovascular pharmacology 75:259-267 PubMed31868825

2019

Restoration of Adiponectin-Connexin43 Signaling Mitigates Myocardial Inflammation and Dysfunction in Diabetic Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Korin E Leffler,Abdel A Abdel-Rahman

Journal of cellular physiology 233:7435-7446 PubMed29663375

2018

Long noncoding RNA H19 mediates LCoR to impact the osteogenic and adipogenic differentiation of mBMSCs in mice through sponging miR-188.

Applications

Unspecified application

Species

Unspecified reactive species

Yijun Wang,Wentao Liu,Yadong Liu,Jianli Cui,Zhiwei Zhao,Hui Cao,Zhuo Fu,Bin Liu

The international journal of biochemistry & cell b 98:29-42 PubMed29471042

2018

Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification.

Applications

Species

Unspecified reactive species

Yuanbo Zhan,Zhiwei He,Xinpeng Liu,Nan Miao,Feng Lin,Wenxia Xu,Sen Mu,Haibin Mu,Mengtong Yuan,Xiaofang Cao,Han Jin,Zhongshuang Liu,Ying Li,Bin Zhang

International journal of molecular medicine 40:713-720 PubMed28731141

2017

Botulinum toxin A improves adipose tissue engraftment by promoting cell proliferation, adipogenesis and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Tang,Chang Chen,Xiaqi Wang,Wei Li,Yan Zhang,Muyao Wang,Wei Jing,Hang Wang,Weihua Guo,Weidong Tian

Journal of cell science 130:1158-1168 PubMed28167681

2017

miR-450a-5p within rat adipose tissue exosome-like vesicles promotes adipogenic differentiation by targeting WISP2.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Mei Yu,Minjia Dai,Chang Chen,Qi Tang,Wei Jing,Hang Wang,Weidong Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Adiponectin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin antibody (AB62551)

Western blot - Anti-Adiponectin antibody (AB62551)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise