Rabbit Recombinant Monoclonal Adiponectin antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications.
Images
![Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347), expandable thumbnail](https://content.abcam.com/products/images/adiponectin-antibody-epr3218-ab133347--western-blot-img8221.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347)")
![Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347), expandable thumbnail](https://content.abcam.com/products/images/adiponectin-antibody-epr3218-ab133347--western-blot-img9463.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347)")
![Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347), expandable thumbnail](https://content.abcam.com/products/images/adiponectin-antibody-epr3218-ab133347--western-blot-img134345.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347)")
![Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347), expandable thumbnail](https://content.abcam.com/products/images/adiponectin-antibody-epr3218-ab133347--western-blot-img7579.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347)")
Publications
Filter by
- Gut microbes 15:22115012023Dietary palmitoleic acid reprograms gut microbiota and improves biological therapy against colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiwei Chen,Qiongdan Mai,Zixu Chen,Tao Lin,Yongjie Cai,Jing Han,Ying Wang,Mudan Zhang,Shimin Tan,Zhiying Wu,Lingming Chen,Zhiyi Zhang,Yi Yang,Taimei Cui,Beiyin Ouyang,Yue Sun,Lijia Yang,Lin Xu,Sien Zhang,Jian Li,Hongbo Shen,Linna Liu,Lingchan Zeng,Shenghong Zhang,Gucheng ZengPubMed 37203220
- Journal of enzyme inhibition and medicinal chemistry 37:1327-13392022Design, synthesis, biological evaluation and QSAR analysis of novel -substituted benzimidazole derived carboxamides.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnja Beč,Marija Mioč,Branimir Bertoša,Marija Kos,Patricia Debogović,Marijeta Kralj,Kristina Starčević,Marijana HranjecPubMed 35514167
- Antioxidants (Basel, Switzerland) 10:2021Low Dietary n6/n3 Ratio Attenuates Changes in the NRF 2 Gene Expression, Lipid Peroxidation, and Inflammatory Markers Induced by Fructose Overconsumption in the Rat Abdominal Adipose Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetra Roškarić,Marcela Šperanda,Tomislav Mašek,Donatella Verbanac,Kristina StarčevićPubMed 34943108
- Journal of biomedical science 25:242018Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Ju Peng et. al.PubMed 29540173
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
Target data
Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Alternative names
Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, ACRP30, apM-1, ACRP30, APM1, ACDC, ADIPOQ, GBP28
Recommended products
Rabbit Recombinant Monoclonal Adiponectin antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR3218
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347), expandable thumbnail](https://content.abcam.com/products/images/adiponectin-antibody-epr3218-ab133347--western-blot-img8221.jpg "Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)")
Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).
All lanes: Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution
Lane 1: Mouse white adipose lysate at 20 µg
Lane 2: Mouse plasma lysate at 20 µg
Lane 3: Rat-1 (Rat embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: Rat white adipose lysate at 20 µg
Lane 5: Rat plasma lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa
![Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347), expandable thumbnail](https://content.abcam.com/products/images/adiponectin-antibody-epr3218-ab133347--western-blot-img9463.jpg "Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)")
Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).
All lanes: Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution
Lane 1: Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Human adipose lysate at 20 µg
Lane 3: Human plasma lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa
![Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347), expandable thumbnail](https://content.abcam.com/products/images/adiponectin-antibody-epr3218-ab133347--western-blot-img134345.jpg "Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)")
Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
All lanes: Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution
Lane 1: Human fat lysate at 10 µg
Lane 2: Human placenta lysate at 10 µg
Lane 3: Human plasma lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
![Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347), expandable thumbnail](https://content.abcam.com/products/images/adiponectin-antibody-epr3218-ab133347--western-blot-img7579.jpg "Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)")
Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
All lanes: Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
Lane 1: Human plasma lysate treated with Beta-ME and heat at 10 µg
Lane 2: Human plasma lysate treated with heat only at 10 µg
Lane 3: Human plasma lysate treated with Beta-ME only at 10 µg
Lane 4: Human plasma lysate untreated at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Downloads
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