Skip to main content

Rabbit Recombinant Monoclonal Adiponectin antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122), expandable thumbnail
  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122), expandable thumbnail
  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122), expandable thumbnail
  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

Alternative names

Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, ACRP30, apM-1, ACRP30, APM1, ACDC, ADIPOQ, GBP28

Recommended products

Rabbit Recombinant Monoclonal Adiponectin antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3218

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab226122 is the carrier-free version of Anti-Adiponectin antibody [EPR3218] ab133347.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.

Biological function summary

Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.

Pathways

Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.

Associated diseases and disorders

Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122), expandable thumbnail

    Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)

    This data was developed using Anti-Adiponectin antibody [EPR3218] ab133347, the same antibody clone in a different buffer formulation.

    The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

    All lanes: Western blot - Anti-Adiponectin antibody [EPR3218] (Anti-Adiponectin antibody [EPR3218] ab133347) at 1/1000 dilution

    Lane 1: Mouse white adipose lysate at 20 µg

    Lane 2: Mouse plasma lysate at 20 µg

    Lane 3: Rat-1 (Rat embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: Rat white adipose lysate at 20 µg

    Lane 5: Rat plasma lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 26 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122), expandable thumbnail

    Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)

    This data was developed using Anti-Adiponectin antibody [EPR3218] ab133347, the same antibody clone in a different buffer formulation.

    The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

    All lanes: Western blot - Anti-Adiponectin antibody [EPR3218] (Anti-Adiponectin antibody [EPR3218] ab133347) at 1/1000 dilution

    Lane 1: Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Human adipose lysate at 20 µg

    Lane 3: Human plasma lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 26 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122), expandable thumbnail

    Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)

    This data was developed using Anti-Adiponectin antibody [EPR3218] ab133347, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122) at 1/1000 dilution

    Lane 1: Human fat lysate at 10 µg

    Lane 2: Human placenta lysate at 10 µg

    Lane 3: Human plasma lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa

  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122), expandable thumbnail

    Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)

    This data was developed using Anti-Adiponectin antibody [EPR3218] ab133347, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)

    Lane 1: Human plasma lysate treated with Beta-ME and heat at 10 µg

    Lane 2: Human plasma lysate treated with heat only at 10 µg

    Lane 3: Human plasma lysate treated with Beta-ME only at 10 µg

    Lane 4: Human plasma lysate untreated at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com