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AB226122

Anti-Adiponectin antibody [EPR3218] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Adiponectin antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

ACDC, ACRP30, APM1, GBP28, ADIPOQ, Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, apM-1

4 Images
Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122)
  • WB

Unknown

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122)

This data was developed using ab133347, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122) at 1/1000 dilution

Lane 1:

Human fat lysate at 10 µg

Lane 2:

Human placenta lysate at 10 µg

Lane 3:

Human plasma lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122)
  • WB

Unknown

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122)

This data was developed using ab133347, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)

Lane 1:

Human plasma lysate treated with Beta-ME and heat at 10 µg

Lane 2:

Human plasma lysate treated with heat only at 10 µg

Lane 3:

Human plasma lysate treated with Beta-ME only at 10 µg

Lane 4:

Human plasma lysate untreated at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122)
  • WB

Unknown

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122)

This data was developed using ab133347, the same antibody clone in a different buffer formulation.

The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID : 15855314, 23967179).

All lanes:

Western blot - Anti-Adiponectin antibody [EPR3218] (<a href='/en-us/products/primary-antibodies/adiponectin-antibody-epr3218-ab133347'>ab133347</a>) at 1/1000 dilution

Lane 1:

Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Human adipose lysate at 20 µg

Lane 3:

Human plasma lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 26 kDa

Observed band size: 30 kDa

false

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122)
  • WB

Unknown

Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (AB226122)

This data was developed using ab133347, the same antibody clone in a different buffer formulation.

The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID : 15855314, 23967179).

All lanes:

Western blot - Anti-Adiponectin antibody [EPR3218] (<a href='/en-us/products/primary-antibodies/adiponectin-antibody-epr3218-ab133347'>ab133347</a>) at 1/1000 dilution

Lane 1:

Mouse white adipose lysate at 20 µg

Lane 2:

Mouse plasma lysate at 20 µg

Lane 3:

Rat-1 (Rat embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

Rat white adipose lysate at 20 µg

Lane 5:

Rat plasma lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 26 kDa

Observed band size: 30 kDa

false

  • Carrier free

    Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (Detector)

  • Unconjugated

    Anti-Adiponectin antibody [EPR3218]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3218

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab226122 is the carrier-free version of ab133347.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.
Biological function summary

Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.

Pathways

Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.

Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
See full target information ADIPOQ
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 25:2152-2165 PubMed39438660

2024

A longitudinal single-cell atlas of anti-tumour necrosis factor treatment in inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Thomas,Matthias Friedrich,Charlotte Rich-Griffin,Mathilde Pohin,Devika Agarwal,Julia Pakpoor,Carl Lee,Ruchi Tandon,Aniko Rendek,Dominik Aschenbrenner,Ashwin Jainarayanan,Alexandru Voda,Jacqueline H Y Siu,Raphael Sanches-Peres,Eloise Nee,Dharshan Sathananthan,Dylan Kotliar,Peter Todd,Maria Kiourlappou,Lisa Gartner,Nicholas Ilott,Fadi Issa,Joanna Hester,Jason Turner,Saba Nayar,Jonas Mackerodt,Fan Zhang,Anna Jonsson,Michael Brenner,Soumya Raychaudhuri,Ruth Kulicke,Danielle Ramsdell,Nicolas Stransky,Ray Pagliarini,Piotr Bielecki,Noah Spies,Brian Marsden,Stephen Taylor,Allon Wagner,Paul Klenerman,Alissa Walsh,Mark Coles,Luke Jostins-Dean,Fiona M Powrie,Andrew Filer,Simon Travis,Holm H Uhlig,Calliope A Dendrou,Christopher D Buckley
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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