JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB50675

Anti-ADIPOR1 antibody [mAbcam50675]

Be the first to review this product! Submit a review

|

(3 Publications)

Mouse Monoclonal ADIPOR1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

PAQR1, TESBP1A, CGI-45, ADIPOR1, Adiponectin receptor protein 1, Progestin and adipoQ receptor family member 1, Progestin and adipoQ receptor family member I

2 Images
Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (AB50675)
  • WB

Lab

Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (AB50675)

Blocking buffer : 3% Milk Gel type : MOPS

All lanes:

Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675) at 10 µg/mL

All lanes:

Human Skeletal Muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/5000 dilution

Predicted band size: 43 kDa

Observed band size: 42 kDa

false

Exposure time: 12min

Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (AB50675)
  • WB

Project

Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (AB50675)

All lanes:

Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675) at 10 µg/mL

All lanes:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 43 kDa

Observed band size: 28 kDa,42 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam50675

Isotype

IgM

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB70362

Anti-ADIPOR1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody (AB70362)

  • 3
  • 9

Complementary Products

Assay Kits

AB65333

Glucose Assay Kit

Functional Studies - Glucose Assay Kit (AB65333)

  • 0
  • 0
Primary Antibodies

AB231051

Anti-Adiponectin Receptor 2/ADIPOR2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adiponectin Receptor 2/ADIPOR2 antibody (AB231051)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB133448

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (AB133448)

  • 4
  • 5
Primary Antibodies

AB133347

Anti-Adiponectin antibody [EPR3218]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Adiponectin antibody [EPR3218] (AB133347)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification technique
Cross-flow filtration
Purification notes
Tissue culture supernatant was concentrated and then buffer exchanged.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adiponectin receptor 1 (ADIPOR1) is a protein that mediates the effects of adiponectin a hormone involved in glucose regulation and fatty acid breakdown. ADIPOR1 is also known as the adiponectin receptor protein 1. It has a molecular weight of approximately 42 kDa. This receptor is widely expressed in various tissues including skeletal muscle liver pancreas and adipose tissue demonstrating its broad role in metabolism.
Biological function summary

ADIPOR1 facilitates the metabolic actions of adiponectin by increasing insulin sensitivity and promoting fatty acid oxidation. The receptor does not function alone; it operates in coordination with another isoform adiponectin receptor 2 (ADIPOR2) forming a complex to exert its full biological effects. This dual receptor system supports the regulation of energy homeostasis and inflammation influencing metabolic rate and improving lipid profiles.

Pathways

ADIPOR1 plays a critical role in the AMP-activated protein kinase (AMPK) pathway and the peroxisome proliferator-activated receptor alpha (PPAR-α) signaling pathway. These pathways are important for cellular energy balance and lipid metabolism. AMPK activation by ADIPOR1 leads to enhanced glucose uptake and fatty acid oxidation while interaction within the PPAR-α pathway regulates gene expression related to lipid metabolism. The activity of ADIPOR1 is intricately tied to other metabolic proteins like leptin and insulin thereby linking with these pathways.

ADIPOR1 involvement is significant in the development of type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). Its interaction with adiponectin and the modulation of insulin sensitivity positions it central to the pathophysiology of these conditions. These interactions highlight its potential as a therapeutic target. Disruption in ADIPOR1 function may influence related proteins such as adiponectin and insulin contributing to the progression of metabolic disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed : 12802337, PubMed : 25855295). Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin (By similarity).
See full target information ADIPOR1
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:5411462 PubMed35996380

2022

Schisandrin A from Schisandra chinensis Attenuates Ferroptosis and NLRP3 Inflammasome-Mediated Pyroptosis in Diabetic Nephropathy through Mitochondrial Damage by AdipoR1 Ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohu Wang,Qin Li,Bangzhi Sui,Maodi Xu,Zhichen Pu,Teng Qiu

Frontiers in physiology 11:655 PubMed32595527

2020

5-Hydroxytryptophan Suppresses the Abdominal Fat Deposit and Is Beneficial to the Intestinal Immune Function in Broilers.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Shaoqiong Liu,Jun Li,Liyuan Wang,Xiaojuan Wang,Jingpeng Zhao,Hongchao Jiao,Hai Lin

Reproductive biology and endocrinology : RB&E 13:81 PubMed26228641

2015

Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys.

Applications

WB

Species

Turkey

Mélodie Diot,Maxime Reverchon,Christelle Rame,Pascal Froment,Jean-Pierre Brillard,Sylvain Brière,Gérard Levêque,Daniel Guillaume,Joëlle Dupont
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com