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AB220738

Anti-Adipose Triglyceride Lipase antibody

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(2 Publications)

Rabbit Polyclonal Adipose Triglyceride Lipase antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PNPLA2 aa 350-500.

View Alternative Names

ATGL, FP17548, PNPLA2, Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Calcium-independent phospholipase A2-zeta, Desnutrin, Pigment epithelium-derived factor receptor, TTS2.2, Transport-secretion protein 2, iPLA2-zeta, PEDF-R, TTS2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase antibody (AB220738)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase antibody (AB220738)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A549 cells labeling Adipose Triglyceride Lipase with ab220738 at 4 μg/ml. Antibody staining is shown in green.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PNPLA2 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

Q96AD5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adipose Triglyceride Lipase often referred to as ATGL is an important enzyme in lipid metabolism. Also known as lipase 29 or desnutrin ATGL hydrolyzes triglycerides into diglycerides and free fatty acids the first step in the breakdown of stored fat. The molecular weight of the ATGL protein is approximately 54 kDa. Cells express ATGL abundantly in adipose tissues but it also appears in cardiac and skeletal muscles highlighting its importance in energy management across different tissues.
Biological function summary

ATGL is part of the lipid mobilization process and contributes significantly to energy homeostasis. It acts distinctively as it breaks down phosphotriglycerides and is not part of a larger complex carrying out its function independently. Its activity impacts the balance of lipid storage and release important for maintaining energy levels within cells and across the entire organism.

Pathways

ATGL plays a significant role in the lipid catabolism and energy regulation pathways. ATGL's activity regulates the fat mobilization pathway by interacting closely with the protein Comparative Gene Identification-58 (CGI-58) which activates it. Further ATGL is involved in the peroxisome proliferator-activated receptor (PPAR) pathway highlighting its role in influencing metabolic processes and energy balance.

ATGL dysregulation connects to metabolic disorders such as obesity and non-alcoholic fatty liver disease (NAFLD). Reduced ATGL activity leads to excessive triglyceride accumulation in adipose tissue contributing to the development of obesity. Moreover ATGL deficiencies can link to cardiac issues through its connection with another lipase Hormone-Sensitive Lipase (HSL) affecting lipid content in cardiac tissues and influencing cardiomyopathies. Understanding ATGL's precise function aids in the development of therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed : 15364929, PubMed : 15550674, PubMed : 16150821, PubMed : 16239926, PubMed : 17603008, PubMed : 34903883). Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade (By similarity). Also possesses acylglycerol transacylase and phospholipase A2 activities (PubMed : 15364929, PubMed : 17032652, PubMed : 17603008). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (PubMed : 17603008). Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed : 16239926). Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes (PubMed : 35676490). Acts antagonistically with LDAH in regulation of cellular lipid stores (PubMed : 28578400). Inhibits LDAH-stimulated lipid droplet fusion (PubMed : 28578400). May play an important role in energy homeostasis (By similarity). May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion (By similarity).
See full target information PNPLA2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2135 PubMed40032835

2025

Proximity proteomics reveals a mechanism of fatty acid transfer at lipid droplet-mitochondria- endoplasmic reticulum contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ayenachew Bezawork-Geleta,Camille J Devereux,Stacey N Keenan,Jieqiong Lou,Ellie Cho,Shuai Nie,David P De Souza,Vinod K Narayana,Nicole A Siddall,Carlos H M Rodrigues,Stephanie Portelli,Tenghao Zheng,Hieu T Nim,Mirana Ramialison,Gary R Hime,Garron T Dodd,Elizabeth Hinde,David B Ascher,David A Stroud,Matthew J Watt

Cellular and molecular biology (Noisy-le-Grand, France) 70:99-109 PubMed38372107

2024

TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyu Sun,Shan Gong,Suwei Lan,Ying He,Yanhua Sun,Zhengmao Zhang
View all publications
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