AB317611

Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Adipose Triglyceride Lipase phospho S406 antibody. Carrier free. Suitable for I-ELISA, WB, IP, ICC/IF and reacts with Synthetic peptide - Human, Synthetic peptide - Mouse, Mouse, Human samples.

View Alternative Names

Atgl, Pnpla2, Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Calcium-independent phospholipase A2-zeta, Desnutrin, iPLA2-zeta

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

This data was developed using ab317610, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling Adipose Triglyceride Lipase with ab317610 at 1/50 (10.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic and membranous staining in differentiated 3T3-L1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Supplier Data

Immunoprecipitation - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

This data was developed using ab317610, the same antibody clone in a different buffer formulation.

Adipose Triglyceride Lipase was immunoprecipitated from 0.35 mg Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate with ab317610 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317610 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
Lane 2 : ab317610 IP in Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317610 in differentiated 3T3-L1 for 6 days whole cell lysate

All lanes:

Immunoprecipitation - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (<a href='/en-us/products/primary-antibodies/adipose-triglyceride-lipase-phospho-s406-antibody-epr27159-29-ab317610'>ab317610</a>) at 1/30 dilution

All lanes:

Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Lab

Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

This data was developed using ab317610, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time :
Lane 1-2 : 5 seconds;
Lane 3-4 : 180 seconds

ab181602 was used as a GAPDH loading control at 1/200000 dilution

All lanes:

Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (<a href='/en-us/products/primary-antibodies/adipose-triglyceride-lipase-phospho-s406-antibody-epr27159-29-ab317610'>ab317610</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate (untreated membrane) at 20 µg

Lane 2:

Human colon tissue lysate (Alkaline phosphatase treated membrane) at 20 µg

Lane 3:

Human fat tissue lysate (untreated membrane) at 20 µg

Lane 4:

Human fat tissue lysate (Alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

Supplier Data

Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

This data was developed using ab317610, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Lane 1:

Undifferentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 40 µg

Lane 2:

3T3-L1 differentiated for 6 days whole cell lysate (untreated membrane) at 40 µg

Lane 3:

Undifferentiated 3T3-L1 whole cell lysate (Lambda phosphatase treated membrane) at 40 µg

Lane 4:

3T3-L1 differentiated for 6 days whole cell lysate (Lambda phosphatase treated membrane) at 40 µg

Lane 5:

Mouse white fat tissue lysate (untreated membrane) at 40 µg

Lane 6:

Mouse white fat tissue lysate (Lambda phosphatase treated membrane) at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,36 kDa

false

Exposure time: 180s

ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

ELISA

Supplier Data

ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

This data was developed using ab317610, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab317610 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Mouse Adipose Triglyceride Lipase(phospho S406),Mouse Adipose Triglyceride Lipase non-phospho,Mouse Adipose Triglyceride Lipase(mutated S406A), Human Adipose Triglyceride Lipase(phospho S404),Human Adipose Triglyceride Lipase non-phospho.

Antigen concentration : 100ng/ml

Unconjugated

Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27159-29

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

I-ELISA, WB, ICC/IF, IP

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes both mouse phospho Ser406 and human phospho Ser404.

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Reactivity data

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Product details

ab317611 is the carrier-free version of ab317610.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adipose Triglyceride Lipase often referred to as ATGL is an important enzyme in lipid metabolism. Also known as lipase 29 or desnutrin ATGL hydrolyzes triglycerides into diglycerides and free fatty acids the first step in the breakdown of stored fat. The molecular weight of the ATGL protein is approximately 54 kDa. Cells express ATGL abundantly in adipose tissues but it also appears in cardiac and skeletal muscles highlighting its importance in energy management across different tissues.

Biological function summary

ATGL is part of the lipid mobilization process and contributes significantly to energy homeostasis. It acts distinctively as it breaks down phosphotriglycerides and is not part of a larger complex carrying out its function independently. Its activity impacts the balance of lipid storage and release important for maintaining energy levels within cells and across the entire organism.

Pathways

ATGL plays a significant role in the lipid catabolism and energy regulation pathways. ATGL's activity regulates the fat mobilization pathway by interacting closely with the protein Comparative Gene Identification-58 (CGI-58) which activates it. Further ATGL is involved in the peroxisome proliferator-activated receptor (PPAR) pathway highlighting its role in influencing metabolic processes and energy balance.

ATGL dysregulation connects to metabolic disorders such as obesity and non-alcoholic fatty liver disease (NAFLD). Reduced ATGL activity leads to excessive triglyceride accumulation in adipose tissue contributing to the development of obesity. Moreover ATGL deficiencies can link to cardiac issues through its connection with another lipase Hormone-Sensitive Lipase (HSL) affecting lipid content in cardiac tissues and influencing cardiomyopathies. Understanding ATGL's precise function aids in the development of therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed : 15337759, PubMed : 15550674, PubMed : 16150821, PubMed : 16675698, PubMed : 16679289, PubMed : 17074755, PubMed : 17114792, PubMed : 23066022). Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade (PubMed : 23066022). Also possesses acylglycerol transacylase and phospholipase A2 activities (By similarity). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (By similarity). Regulates adiposome size and may be involved in the degradation of adiposomes (By similarity). Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes (PubMed : 35676490). Acts antagonistically with LDAH in regulation of cellular lipid stores (By similarity). Inhibits LDAH-stimulated lipid droplet fusion (By similarity). May play an important role in energy homeostasis (PubMed : 16675698). May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion (PubMed : 15337759).

See full target information Pnpla2 phospho S406

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

Immunoprecipitation - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] - BSA and Azide free (AB317611)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise