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Rabbit Recombinant Monoclonal ADK antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Rat, Mouse, Human samples.

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Images

Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (AB307357), expandable thumbnail
  • Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (AB307357), expandable thumbnail
  • Western blot - Anti-ADK antibody [EPR27497-67] (AB307357), expandable thumbnail
  • Western blot - Anti-ADK antibody [EPR27497-67] (AB307357), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] (AB307357), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-FrIHC-PWB
Human
Not recommended
Tested
Tested
Not recommended
Not recommended
Tested
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Rat

Dilution info

1/30

Notes

-

Species

Mouse

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50.00000 - 1/1000.00000

Notes

-

Species

Human

Dilution info

1/50.00000 - 1/1000.00000

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Target data

Function

Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

Alternative names

Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK

Recommended products

Rabbit Recombinant Monoclonal ADK antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27497-67

Purification technique

Affinity purification Protein A

Specificity

This antibody is unsuitable for human IP and rat ICC.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Adenosine kinase (ADK) also known by its alternative name ATP:adenosine 5'-phosphotransferase is an enzyme with a molecular mass of approximately 40 kDa. This enzyme catalyzes the transfer of a phosphate group from ATP to adenosine forming AMP. ADK is expressed in several tissues with significant presence in the liver brain and lungs. This distribution suggests its versatile role in organ-specific metabolism and regulatory processes.

Biological function summary

The enzyme modulates cellular adenosine levels which influences various physiological functions such as sleep regulation and immune responses. ADK does not operate as part of a larger protein complex but acts independently to maintain intracellular energy balance by regulating the pools of adenosine and AMP. The enzyme activity affects purine metabolism important in diverse cellular activities.

Pathways

The activity of adenosine kinase fits prominently into the purine metabolism and energy homeostasis pathways. Its function maintains the cellular concentrations of adenosine a nucleotide affecting cellular signaling pathways including the AMP-activated protein kinase (AMPK) pathway. ADK interacts with enzymes like adenosine deaminase which collectively orchestrate the purine salvage pathway—pivotal in nucleotide recycling.

Associated diseases and disorders

Irregularities in ADK function have implications in neurological disorders and liver diseases. Abnormal ADK activity links to epilepsy due to its role in controlling adenosine concentrations affecting neurotransmitter release. Liver diseases such as hepatic steatosis have shown associations with altered ADK function impacting energy balance. The interplay with proteins such as AMP-activated protein kinase indicates potential therapeutic targets in disorders where metabolic regulation is compromised.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (ab307357), expandable thumbnail

    Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (ab307357)

    ADK was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate 10 µg with ab307357 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307357 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: C6 whole cell lysate 10 µg

    Lane 2: ab307357 IP in C6 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307357 in C6 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 128 seconds.

    All lanes: Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (ab307357) at 1/1000 dilution

    Lane 1: C6 (rat glial tumor glial cell) whole cell lysate

    Lane 2: C6 whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 45 kDa

    Exposure time: 128s

  • Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (ab307357), expandable thumbnail

    Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (ab307357)

    ADK was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 µg with ab307357 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307357 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: RAW264.7 whole cell lysate 10 µg

    Lane 2: ab307357 IP in RAW264.7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307357 in RAW264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 128 seconds

    All lanes: Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (ab307357) at 1/1000 dilution

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lane 2: RAW264.7 whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 45 kDa

    Exposure time: 128s

  • Western blot - Anti-ADK antibody [EPR27497-67] (ab307357), expandable thumbnail

    Western blot - Anti-ADK antibody [EPR27497-67] (ab307357)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: A549

    In Western blot, ab307357 was shown to bind specifically to ADK. A band was observed at 45 kDa in wild-type HAP1 cell lysates whereas no signal observed at this size in ADK knockout cell line. 

    This blot (lanes 1-4) was developed using a high sensitivity ECL substrate.

    Exposure time: 125 seconds

    All lanes: Western blot - Anti-ADK antibody [EPR27497-67] (ab307357) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate

    Lane 2: ADK knockout HAP1 whole cell lysate

    Lane 3: HeLa whole cell lysate

    Lane 4: A549 whole cell lysate

    Lane 5: RAW264.7 whole cell lysate

    Lane 6: C6 whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 45 kDa

    Exposure time: 125s

  • Western blot - Anti-ADK antibody [EPR27497-67] (ab307357), expandable thumbnail

    Western blot - Anti-ADK antibody [EPR27497-67] (ab307357)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: mouse heart (PMID:15930047;14736855), rat heart (PMID:15930047;14736855).

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-ADK antibody [EPR27497-67] (ab307357) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate

    Lane 2: Rat liver tissue lysate

    Lane 3: Mouse heart tissue lysate

    Lane 4: Rat heart tissue lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45 kDa

    Exposure time: 15s

  • Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] (ab307357), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] (ab307357)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling ADK with ab307357 at 1/50 dilution (10.64 µg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
    Confocal image showing nuclear and weak cytoplasmic staining in NIH/3T3 cells.
    The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

  • Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] (ab307357), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] (ab307357)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ADK KO HAP1 (ADK knockout human chronic myelogenous leukemia near-haploid cell) cells labeling ADK with ab307357 at 1/50 dilution (10.64 µg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
    Confocal image showing nuclear and weak cytoplasmic staining in wildtype HAP1 cells, and no staining in ADK knockout HAP1 cells.
    The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

  • Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] (ab307357), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] (ab307357)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) (Right) / ADK knockout HAP1 (Left) cells labeling ADK with ab307357 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] (ab307357), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] (ab307357)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling ADK with ab307357 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

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