Rabbit Recombinant Monoclonal ADK antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--immunoprecipitation-img406389.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358)")
![Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--immunoprecipitation-img406390.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358)")
![Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--western-blot-img405401.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358)")
![Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--western-blot-img405400.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358)")
![Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--immunocytochemistry-immunofluorescence-img405397.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (AB307358)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Not recommended | Not recommended | Tested |
| Mouse | Tested | Tested | Expected | Not recommended | Not recommended | Tested |
| Rat | Tested | Expected | Expected | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species Mouse | Dilution info 1/30 | Notes - |
Species Rat | Dilution info 1/30 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species Mouse | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50 | Notes - |
Species Mouse | Dilution info 1/50 | Notes - |
Species Human | Dilution info 1/50 | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Alternative names
Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK
Recommended products
Rabbit Recombinant Monoclonal ADK antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse, Rat samples.
Alternative names
Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR27497-67
- Purification technique
Affinity purification Protein A
- Specificity
This antibody is unsuitable for human IP and rat ICC/IF.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Adenosine kinase (ADK) also known by its alternative name ATP:adenosine 5'-phosphotransferase is an enzyme with a molecular mass of approximately 40 kDa. This enzyme catalyzes the transfer of a phosphate group from ATP to adenosine forming AMP. ADK is expressed in several tissues with significant presence in the liver brain and lungs. This distribution suggests its versatile role in organ-specific metabolism and regulatory processes.
- Biological function summary
The enzyme modulates cellular adenosine levels which influences various physiological functions such as sleep regulation and immune responses. ADK does not operate as part of a larger protein complex but acts independently to maintain intracellular energy balance by regulating the pools of adenosine and AMP. The enzyme activity affects purine metabolism important in diverse cellular activities.
- Pathways
The activity of adenosine kinase fits prominently into the purine metabolism and energy homeostasis pathways. Its function maintains the cellular concentrations of adenosine a nucleotide affecting cellular signaling pathways including the AMP-activated protein kinase (AMPK) pathway. ADK interacts with enzymes like adenosine deaminase which collectively orchestrate the purine salvage pathway—pivotal in nucleotide recycling.
- Associated diseases and disorders
Irregularities in ADK function have implications in neurological disorders and liver diseases. Abnormal ADK activity links to epilepsy due to its role in controlling adenosine concentrations affecting neurotransmitter release. Liver diseases such as hepatic steatosis have shown associations with altered ADK function impacting energy balance. The interplay with proteins such as AMP-activated protein kinase indicates potential therapeutic targets in disorders where metabolic regulation is compromised.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--immunoprecipitation-img406389.jpg "Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)
This data was developed using Anti-ADK antibody [EPR27497-67] ab307357, the same antibody clone in a different buffer formulation.
ADK was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate 10 µg with Anti-ADK antibody [EPR27497-67] ab307357 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ADK antibody [EPR27497-67] ab307357 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: C6 whole cell lysate 10 µg
Lane 2: Anti-ADK antibody [EPR27497-67] ab307357 IP in C6 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADK antibody [EPR27497-67] ab307357 in C6 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 128 seconds.All lanes: Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (Anti-ADK antibody [EPR27497-67] ab307357) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell) whole cell lysate
Lane 2: C6 whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 45 kDa
Exposure time: 128s
ADK was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate 10 µg with Anti-ADK antibody [EPR27497-67] ab307357 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ADK antibody [EPR27497-67] ab307357 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: C6 whole cell lysate 10 µg
Lane 2: Anti-ADK antibody [EPR27497-67] ab307357 IP in C6 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADK antibody [EPR27497-67] ab307357 in C6 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 128 seconds.![Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--immunoprecipitation-img406390.jpg "Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
Immunoprecipitation - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)
This data was developed using Anti-ADK antibody [EPR27497-67] ab307357, the same antibody clone in a different buffer formulation.
ADK was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 µg with Anti-ADK antibody [EPR27497-67] ab307357 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ADK antibody [EPR27497-67] ab307357 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW264.7 whole cell lysate 10 µg
Lane 2: Anti-ADK antibody [EPR27497-67] ab307357 IP in RAW264.7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADK antibody [EPR27497-67] ab307357 in RAW264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 128 secondsAll lanes: Immunoprecipitation - Anti-ADK antibody [EPR27497-67] (Anti-ADK antibody [EPR27497-67] ab307357) at 1/1000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2: RAW264.7 whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 45 kDa
Exposure time: 128s
ADK was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 µg with Anti-ADK antibody [EPR27497-67] ab307357 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ADK antibody [EPR27497-67] ab307357 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW264.7 whole cell lysate 10 µg
Lane 2: Anti-ADK antibody [EPR27497-67] ab307357 IP in RAW264.7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADK antibody [EPR27497-67] ab307357 in RAW264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 128 seconds![Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--western-blot-img405401.jpg "Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)
This data was developed using 307357, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Low expression: A549
In Western blot, Anti-ADK antibody [EPR27497-67] ab307357 was shown to bind specifically to ADK. A band was observed at 45 kDa in wild-type HAP1 cell lysates whereas no signal observed at this size in ADK knockout cell line.
This blot (lanes 1-4) was developed using a high sensitivity ECL substrate.
125 seconds
Exposure time:All lanes: Western blot - Anti-ADK antibody [EPR27497-67] (Anti-ADK antibody [EPR27497-67] ab307357) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate
Lane 2: ADK knockout HAP1 whole cell lysate
Lane 3: HeLa whole cell lysate
Lane 4: A549 whole cell lysate
Lane 5: RAW264.7 whole cell lysate
Lane 6: C6 whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 45 kDa
Exposure time: 125s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: A549
In Western blot, Anti-ADK antibody [EPR27497-67] ab307357 was shown to bind specifically to ADK. A band was observed at 45 kDa in wild-type HAP1 cell lysates whereas no signal observed at this size in ADK knockout cell line.
This blot (lanes 1-4) was developed using a high sensitivity ECL substrate.
Exposure time: 125 seconds![Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--western-blot-img405400.jpg "Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
Western blot - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)
This data was developed using 307357, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: mouse heart (PMID:15930047;14736855), rat heart (PMID:15930047;14736855).
Exposure time: 15 secondsAll lanes: Western blot - Anti-ADK antibody [EPR27497-67] (Anti-ADK antibody [EPR27497-67] ab307357) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Mouse heart tissue lysate
Lane 4: Rat heart tissue lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 45 kDa
Exposure time: 15s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: mouse heart (PMID:15930047;14736855), rat heart (PMID:15930047;14736855).
Exposure time: 15 seconds![Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--immunocytochemistry-immunofluorescence-img405397.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)
This data was developed using Anti-ADK antibody [EPR27497-67] ab307357, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling ADK with Anti-ADK antibody [EPR27497-67] ab307357 at 1/50 dilution (10.64 µg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing nuclear and weak cytoplasmic staining in NIH/3T3 cells.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).![Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--immunocytochemistry-immunofluorescence-img405396.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)
This data was developed using Anti-ADK antibody [EPR27497-67] ab307357, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ADK KO HAP1 (ADK knockout human chronic myelogenous leukemia near-haploid cell) cells labeling ADK with Anti-ADK antibody [EPR27497-67] ab307357 at 1/50 dilution (10.64 µg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing nuclear and weak cytoplasmic staining in wildtype HAP1 cells, and no staining in ADK knockout HAP1 cells.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).![Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--flow-cytometry-intracellular-img405395.jpg "Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)
This data was developed using Anti-ADK antibody [EPR27497-67] ab307357, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) (Right) / ADK knockout HAP1 (Left) cells labeling ADK with Anti-ADK antibody [EPR27497-67] ab307357 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody.![Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--flow-cytometry-intracellular-img405394.jpg "Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)
This data was developed using Anti-ADK antibody [EPR27497-67] ab307357, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling ADK with Anti-ADK antibody [EPR27497-67] ab307357 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--immunocytochemistry-immunofluorescence-img405396.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
![Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--flow-cytometry-intracellular-img405395.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
![Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358), expandable thumbnail](https://content.abcam.com/products/images/adk-antibody-epr27497-67-bsa-and-azide-free-ab307358--flow-cytometry-intracellular-img405394.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ADK antibody [EPR27497-67] - BSA and Azide free (ab307358)")
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