Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)

Rabbit Monoclonal ADNP antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (AB300115), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	ICC/IF	Flow Cyt (Intra)	IP	ChIP
Human	Tested	Tested	Expected	Tested	Tested	Tested	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested	Tested	Not recommended
Rat	Tested	Tested	Tested	Expected	Expected	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information ADNP

Function

May be involved in transcriptional regulation. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation. Positively modulates WNT-beta-catenin/CTNN1B signaling, acting by regulating phosphorylation of, and thereby stabilizing, CTNNB1. May be required for neural induction and neuronal differentiation. May be involved in erythroid differentiation (By similarity).

Alternative names

ADNP1, KIAA0784, ADNP, Activity-dependent neuroprotector homeobox protein, Activity-dependent neuroprotective protein

Recommended products

Rabbit Monoclonal ADNP antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25434-4
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Aliquoting information: Upon delivery aliquot

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ADNP protein also known as Activity-dependent neuroprotector homeobox is a multifunctional protein with a mass of about 123 kDa. ADNP is highly expressed in the brain but also present in other tissues including heart and kidney. It regulates neural development and synaptic plasticity by interacting with chromatin and participating in transcriptional activities. The protein plays a role in cell cycle regulation and is involved in neuroprotection against multiple insults.

Biological function summary

ADNP plays critical roles in the development of the nervous system and maintenance of neuronal function. As part of a chromatin remodeling complex known as SWI/SNF it modulates the expression of genes necessary for neurogenesis and neuronal survival. ADNP affects microtubule dynamics which are important for neuron structure and transport by regulating activity of associated proteins. These functions make ADNP influential in maintaining a healthy nervous system and protecting against neurodegenerative processes.

Pathways

ADNP is necessary for proper functioning in neuroprotective pathways and the regulation of gene expression pathways. It closely interacts with the proteins REST and HDAC2 in the regulation of transcription important for neuronal cell survival. The involvement in Wnt signaling pathway an important regulator of cell proliferation and differentiation highlights its importance in neural development and plasticity. ADNP also affects the activity of MAPK/ERK pathway which links it to the control of cell proliferation and survival.

Associated diseases and disorders

ADNP mutations or dysregulations have been connected to neurodevelopmental disorders such as Helsmoortel-Van der Aa syndrome and Alzheimer's disease. In relation to Helsmoortel-Van der Aa syndrome disruptions in ADNP lead to intellectual disability and developmental delays due to impaired neural growth. In Alzheimer's disease changes in ADNP expression affect the stability and function of tau a protein involved in neurofibrillary tangles. These interactions underline ADNP's significance in neuropsychiatric disorders and potential as a therapeutic target.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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17 product images

Western blot - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
This data was developed using Anti-ADNP antibody [EPR25434-4] ab300114, the same antibody clone in a different buffer formulation.
Anti-ADNP antibody [EPR25434-4] ab300114 was shown to react with ADNP in wild-type DMS 53 cells in Western blot with loss of signal observed in a ADNP siRNA knockdown cell line. Cell lysates from wild-type DMS 53 transfected with either scrambled siRNA or ADNP siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-ADNP antibody [EPR25434-4] ab300114 overnight at 4 °C at a 1/10000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-ADNP antibody [EPR25434-4] (Anti-ADNP antibody [EPR25434-4] ab300114) at 1/10000 dilution
Lane 1: Wild-type DMS 53 transfected with scrambled siRNA control lysate at 30 µg
Lane 2: DMS 53 transfected with siRNA specifically targeting ADNP cell lysate at 30 µg
Immunocytochemistry/ Immunofluorescence - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
This data was developed using Anti-ADNP antibody [EPR25434-4] ab300114, the same antibody clone in a different buffer formulation.
Anti-ADNP antibody [EPR25434-4] ab300114 was shown to react with ADNP in wild-type DMS 53 cells in immunocytochemistry with loss of signal observed in a ADNP siRNA knockdown cell line. Wild-type and siRNA knockdown cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1x PBS, 0.01% Triton X-100, 5% BSA, 5% NGS. The cells were then incubated with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/500 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor® 555) at 0.5 ?g/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (siRNA knockdown) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and siRNA knockdown cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
Immunoprecipitation - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
All lanes: Immunoprecipitation - Anti-ADNP antibody [EPR25434-4] (Anti-ADNP antibody [EPR25434-4] ab300114) at 2 µg
Lanes 1 and 3: DMS 53 cells
Lane 2: Unbound fraction: Rabbit monoclonal IgG instead of Anti-ADNP antibody [EPR25434-4] ab300114 in DMS 53 cells
Immunohistochemistry (Frozen sections) - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). PBS was used instead of Anti-ADNP antibody [EPR25434-4] ab300114 followed by preabsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/500 dilution (Red) compared with a rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed and 90% methnol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/500 dilution (Red) compared with a rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: PBS was used instead of Anti-ADNP antibody [EPR25434-4] ab300114 followed by preadsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunocytochemistry - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
This data was developed using Anti-ADNP antibody [EPR25434-4] ab300114, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized NIH/3T3 (mouse embryonic fibroblast) cells lebelling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/50 (11.22 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/mL) dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody control: PBS was used instead of Anti-ADNP antibody [EPR25434-4] ab300114 followed by preabsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/mL) dilution.
Immunocytochemistry - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized HeLa (human cervix adenocarcinoma epithelial cell) cells lebelling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. Tubuline was stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: PBS was used instead of Anti-ADNP antibody [EPR25434-4] ab300114 followed by preabsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat cerebrum is observed. The section was incubated with Anti-ADNP antibody [EPR25434-4] ab300114 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Immunohistochemical analysis of paraffin-embedded mouse glioblastoma tissue labelling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse glioblastoma is observed. The section was incubated with Anti-ADNP antibody [EPR25434-4] ab300114 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on mouse cerebrum is observed. The section was incubated with Anti-ADNP antibody [EPR25434-4] ab300114 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling ADNP with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human colon carcinoma is observed (PMID: 27903678). The section was incubated with Anti-ADNP antibody [EPR25434-4] ab300114 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Blocking and dilution buffer and concentration: 5% NFDM/TBST. Lysates should be made freshly and used in WB immediately to minimize protein degradation. We used fresh mouse brain tissue lysate. This blot was developed using a higher sensitivity ECL substrate.
All lanes: Western blot - Anti-ADNP antibody [EPR25434-4] (Anti-ADNP antibody [EPR25434-4] ab300114) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 3s
Western blot - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
Blocking and dilution buffer and concentration: 5% NFDM/TBST. Lysates should be made freshly and used in WB immediately to minimize protein degradation.
All lanes: Western blot - Anti-ADNP antibody [EPR25434-4] (Anti-ADNP antibody [EPR25434-4] ab300114) at 1/1000 dilution
Lane 1: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
Lane 3: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 3s
Immunoprecipitation - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
ADNP was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/30 dilution. Western blot was performed on the immunoprecipitate using Anti-ADNP antibody [EPR25434-4] ab300114 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1 (Input): NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 µg. Lane 2 (+): Anti-ADNP antibody [EPR25434-4] ab300114 IP in NIH/3T3 whole cell lysate. Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADNP antibody [EPR25434-4] ab300114 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-ADNP antibody [EPR25434-4] (Anti-ADNP antibody [EPR25434-4] ab300114) at 1/30 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Lane 2: Anti-ADNP antibody [EPR25434-4] ab300114 IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADNP antibody [EPR25434-4] ab300114 in NIH/3T3 whole cell lysate
Immunoprecipitation - Anti-ADNP antibody [EPR25434-4] - BSA and Azide free (ab300115)
ADNP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-ADNP antibody [EPR25434-4] ab300114 at 1/30 dilution. Western blot was performed on the immunoprecipitate using Anti-ADNP antibody [EPR25434-4] ab300114 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1 (Input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg. Lane 2 (+): Anti-ADNP antibody [EPR25434-4] ab300114 IP in HeLa whole cell lysate. Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADNP antibody [EPR25434-4] ab300114 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 84 seconds
All lanes: Immunoprecipitation - Anti-ADNP antibody [EPR25434-4] (Anti-ADNP antibody [EPR25434-4] ab300114) at 1/30 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg
Lane 2: Anti-ADNP antibody [EPR25434-4] ab300114 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADNP antibody [EPR25434-4] ab300114 in HeLa whole cell lysate