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AB248683

Anti-ADO antibody [EPR6581] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ADO antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C10orf22, ADO, 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase

1 Images
Western blot - Anti-ADO antibody [EPR6581] - BSA and Azide free (AB248683)
  • WB

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Western blot - Anti-ADO antibody [EPR6581] - BSA and Azide free (AB248683)

Blocking/Diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134102).

All lanes:

Western blot - Anti-ADO antibody [EPR6581] (<a href='/en-us/products/primary-antibodies/ado-antibody-epr6581-ab134102'>ab134102</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

A-673 (Human muscle Ewing's Sarcoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 28-30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6581

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248683 is the carrier-free version of ab134102.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine deaminase (ADO) also known as ADA is an enzyme that plays an important role in the metabolism of purine. The molecular weight of ADO approximately 41 kDa allows it to facilitate the deamination of adenosine into inosine an essential step in nucleoside metabolism. ADO is expressed in various tissues including the lymphoid tissues and red blood cells where its enzymatic activity is necessary for maintaining cellular homeostasis.
Biological function summary

The activity of adenosine deaminase is significant for the development and function of the immune system. ADO does not act alone but is part of a larger enzymatic framework involved in nucleic acid metabolism. It helps regulate levels of adenosine and other deaminated derivatives and is important in cells where rapid proliferation occurs such as in lymphocytes. ADO interacts with proteins in this metabolic complex to manage the pool of nucleosides available to the cell which influences cell signaling processes.

Pathways

Adenosine deaminase integrates into the purine salvage pathway essential for the recycling and management of purine nucleotides in cellular metabolism. Within this framework ADO interacts with purine nucleoside phosphorylase (PNP) which further processes inosine to hypoxanthine continuing the nucleotide cycle. These processes are necessary for maintaining cellular function and survival especially under conditions that demand rapid cell proliferation or repair.

Adenosine deaminase deficiency is linked to severe combined immunodeficiency (SCID) a condition that severely impairs the immune system. This deficiency leads to the accumulation of toxic levels of adenosine and deoxyadenosine damaging lymphocytes. The enzyme's connection to diseases also includes interactions with proteins such as RAG1 and RAG2 which are important for lymphocyte development. Additionally overactivity of ADO has implications in chronic inflammatory diseases linking it to pathological conditions where purine metabolism becomes dysregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a vital role in regulating thiol metabolism and preserving oxygen homeostasis by oxidizing the sulfur of cysteamine and N-terminal cysteine-containing proteins to their corresponding sulfinic acids using O2 as a cosubstrate (PubMed : 17581819, PubMed : 29752763, PubMed : 31273118, PubMed : 32601061). Catalyzes the oxidation of cysteamine (2-aminoethanethiol) to hypotaurine (PubMed : 17581819, PubMed : 29752763, PubMed : 32601061). Catalyzes the oxidation of regulators of G-protein signaling 4 (RGS4) and 5 (RGS5) and interleukin-32 (IL32) (PubMed : 31273118, PubMed : 32601061).
See full target information ADO
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:4513-4535 PubMed35832075

2022

Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,An-Dong Liu,Qian Niu,Xiao Feng,Yuan-Yi Zheng,Shuai-Jun Chen,Hui Xu,Qian Li,Guo-Qing Hou,Xiao-Yang Bi,Yu-Zhi Lu,Pei-Pei Cheng,Li-Mei Liang,Ye-Han Jiang,Li-Qin Zhao,Fei Liu,Lin-Jie Song,Li-Ling Zhou,Ling-Yan Xiao,Feng Chen,Shawn Shun-Cheng Li,Wan-Li Ma,Xuan Cao,Hong Ye
View all publications
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