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AB169523

Anti-ADRA1B antibody [EPR10336]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • What is this?

1

(1 Review)

|

(25 Publications)

Rabbit Recombinant Monoclonal ADRA1B antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 25 publications.

View Alternative Names

Alpha-1B adrenergic receptor, Alpha-1B adrenoreceptor, Alpha-1B adrenoceptor, ADRA1B

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (AB169523)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (AB169523)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining predominantly on collecting duct of human kidney. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (AB169523)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (AB169523)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human ovarian carcinoma. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (AB169523)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (AB169523)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat stomach. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (AB169523)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRA1B antibody [EPR10336] (AB169523)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse stomach. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.

Western blot - Anti-ADRA1B antibody [EPR10336] (AB169523)
  • WB

Lab

Western blot - Anti-ADRA1B antibody [EPR10336] (AB169523)

All lanes:

Western blot - Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/2000 dilution

Lane 1:

Human fetal brain lysates at 20 µg

Lane 2:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 5:

Rat brain lysates at 20 µg

Lane 6:

Mouse stomach lysates at 20 µg

Lane 7:

Rat stomach lysates at 20 µg

Lane 8:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Western blot - Anti-ADRA1B antibody [EPR10336] (AB169523)
  • WB

Unknown

Western blot - Anti-ADRA1B antibody [EPR10336] (AB169523)

All lanes:

Western blot - Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

PC-3 lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

false

  • Carrier free

    Anti-ADRA1B antibody [EPR10336] - BSA and Azide free

  • HRP

    HRP Anti-ADRA1B antibody [EPR10336]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10336

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100 - 1/500", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100 - 1/500", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100 - 1/500", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADRA1B also known as alpha-1B adrenergic receptor is a G protein-coupled receptor (GPCR) involved in various cellular processes. It has a molecular mass of approximately 56 kDa. ADRA1B mainly expresses in smooth muscle cells particularly within arterial walls and cardiac tissues which suggests its involvement in cardiovascular function. It also expresses in the central nervous system reflecting a broader physiological role.
Biological function summary

Alpha-1B adrenergic receptor mediates vasoconstriction and regulates blood pressure by interacting with catecholamines like norepinephrine. This receptor participates in forming a homodimer or a heterodimer complex with other adrenergic receptors which affects its signaling capabilities and functional outcomes. The receptor's activation influences smooth muscle contraction cardiac output and alpha-adrenergic signaling.

Pathways

ADRA1B actively participates in the adrenergic signaling pathway influencing cardiovascular regulation and smooth muscle contraction. The receptor interacts with proteins such as G proteins which are central to signal transduction. It also relates to the mitogen-activated protein kinase (MAPK) pathway promoting cell proliferation and survival linking its function to both cardiac and neural activities.

ADRA1B associates with hypertension and heart failure where its overexpression or dysregulation affects vascular resistance and cardiac workload. It connects to proteins like phospholipase C which further modulate intracellular calcium levels linked to these cardiovascular conditions. Additionally changes in this receptor's function may relate to psychiatric disorders reflecting its role in the central nervous system and interactions with noradrenergic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Alpha-1 adrenergic receptors are G protein-coupled receptors for catecholamines that signal through the G(q) family of G proteins, including G(q) and G(11). Upon activation, they stimulate the phosphatidylinositol-calcium second messenger pathway, leading to calcium release from intracellular stores and activation of protein kinase C (By similarity). ADRA1B binds the catecholamine ligands norepinephrine and epinephrine (PubMed : 7815325, PubMed : 8183249). Can also couple to G(14) and G(16) proteins (By similarity). Nuclear ADRA1B forms heterooligomers with ADRA1A to regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes (PubMed : 18802028, PubMed : 22120526). At the plasma membrane, ADRA1B interacts with CAVIN4/MURC to regulates ERK activation in cardiomyocytes, contributing to the regulation of cardiac hypertrophy (PubMed : 24567387).
See full target information ADRA1B
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 601:1383-1405 PubMed36864773

2023

Short-term high-fat diet feeding of mice suppresses catecholamine-stimulated Ca signalling in hepatocytes and intact liver.

Applications

Unspecified application

Species

Unspecified reactive species

Robert P Brumer,Juliana C Corrêa-Velloso,Samantha J Thomas,Oleta A Sandiford,Andrew P Thomas,Paula J Bartlett

Cells 12: PubMed36831252

2023

Alpha1A- and Beta3-Adrenoceptors Interplay in Adipose Multipotent Mesenchymal Stromal Cells: A Novel Mechanism of Obesity-Driven Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Vadim Chechekhin,Anastasia Ivanova,Konstantin Kulebyakin,Veronika Sysoeva,Daria Naida,Mikhail Arbatsky,Nataliya Basalova,Maxim Karagyaur,Mariya Skryabina,Anastasia Efimenko,Olga Grigorieva,Natalia Kalinina,Vsevolod Tkachuk,Pyotr Tyurin-Kuzmin

American journal of physiology. Lung cellular and molecular physiology 324:L639-L651 PubMed36648147

2023

α1 Adrenoreceptor antagonism mitigates extracellular mitochondrial DNA accumulation in lung fibrosis models and in patients with idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Genta Ishikawa,Xueyan Peng,John McGovern,Sam Woo,Carrighan Perry,Angela Liu,Sheeline Yu,Alexander Ghincea,Aliaksandr Kishchanka,Vitória Fiorini,Buqu Hu,Ying Sun,Huanxing Sun,Changwan Ryu,Erica L Herzog

FEBS letters 596:2706-2716 PubMed35920096

2022

Heteromerization between α -adrenoceptor and chemokine (C-C motif) receptor 2 biases α -adrenoceptor signaling: Implications for vascular function.

Applications

Unspecified application

Species

Unspecified reactive species

Xianlong Gao,Anthony J DeSantis,Garrett A Enten,McWayne Weche,Jorge E Marcet,Matthias Majetschak

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

Proceedings of the National Academy of Sciences of the United States of America 119:e2123511119 PubMed35537053

2022

α-adrenoceptors regulate chemokine receptor-mediated leukocyte migration via formation of heteromeric receptor complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Garrett A Enten,Xianlong Gao,Hannah R Strzelinski,McWayne Weche,Stephen B Liggett,Matthias Majetschak

PloS one 16:e0253821 PubMed34166476

2021

Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren J Albee,Xianlong Gao,Matthias Majetschak

The Journal of endocrinology 248:277-288 PubMed33434145

2021

Norepinephrine exposure restrains endometrial decidualization during early pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiju Wang,Yunhui Tang,Songcun Wang,Liyuan Cui,Dajin Li,Meirong Du

The Journal of clinical investigation 131: PubMed33393489

2021

Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijuan Gao,Xueyan Peng,Carrighan Perry,Huanxing Sun,Aglaia Ntokou,Changwan Ryu,Jose L Gomez,Benjamin C Reeves,Anjali Walia,Naftali Kaminski,Nir Neumark,Genta Ishikawa,Katharine E Black,Lida P Hariri,Meagan W Moore,Mridu Gulati,Robert J Homer,Daniel M Greif,Holger K Eltzschig,Erica L Herzog

Journal of cellular and molecular medicine : PubMed33118708

2020

Alterations in the phosphodiesterase type 5 pathway and oxidative stress correlate with erectile function in spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Weixiang He,Jianmin Liu,Daoquan Liu,Jundong Hu,Ye Jiang,Mingzhou Li,Qian Wang,Ping Chen,Guang Zeng,Deqiang Xu,Xinghuan Wang,Michael E DiSanto,Xinhua Zhang
View all publications
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Product promise

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