Anti-ADRM1/ARM-1 antibody [EPR11450(B)]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (AB157218)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ADRM1/ARM-1 with ab157218 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (AB157218)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling ADRM1/ARM-1 with ab157218 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (AB157218)

Intracellular flow cytometric analysis of permeabilized Hela cells using ab157218 at 1/10 (red) or a rabbit IgG negative (green).

• WB

Lab

Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (AB157218)

Lanes 1-4 : Merged signal (red and green). Green - ab157218 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.

ab157218 Anti-ADRM1/ARM-1 antibody [EPR11450(B)] was shown to specifically react with ADRM1/ARM-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266765 (knockout cell lysate ab257816) was used. Wild-type and ADRM1/ARM-1 knockout samples were subjected to SDS-PAGE. ab157218 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ADRM1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ADRM1 (ARM-1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-adrm1-arm-1-knockout-hek-293t-cell-line-ab266765'>ab266765</a>)

Lane 3:

K-562 cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

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• WB

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Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (AB157218)

All lanes:

Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

RAW 264.7 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Predicted band size: 42 kDa

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• WB

CiteAb

Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (AB157218)

ADRM1/ARM-1 western blot using anti-ADRM1/ARM-1 antibody [EPR11450(B)] ab157218. Publication image and figure legend from Feng, Q., Yao, J., et al., 2018, Front Immunol, PubMed 30177931.

ab157218 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab157218 please see the product overview.

ARC downregulated apoptosis and autophagy in ConA-induced hepatitis. (A) The expressions of Bnip3, Beclin1, LC3B, p62, and Adrm1 were measured by IHC. (B) The protein levels of Bnip3, Beclin1, LC3B, p62, and Adrm1 were measured by Western blot. The values are represented as mean ± SD. **p < 0.01 compared with model group (n = 8). The mice pre-treated with vehicle were used as model group.

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