Anti-ADX antibody [EPR29804-54] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ADX with ab321899 at 1/500 (0.976 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human cerebrum. The section was incubated with ab321899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling ADX with ab321899 at 1/2000 (0.244 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human adrenal gland (A) at 1/500 dilution (0.976 ug/mL) and (B) at 1/2000 dilution (0.244 ug/mL). The section was incubated with ab321899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ADX with ab321899 at 1/500 (0.976 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human testis. The section was incubated with ab321899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADX with ab321899 at 1/500 (0.976 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney. The section was incubated with ab321899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse adrenal gland staining of ADX with ab321899 at a 1/2000 (0.244 µg/ml) dilution VGF with ab308287 at 1/500 (0.95 µg/ml) dilution and Neuropeptide Y with ab221145 at 1/4000 ( 0.140 µg/ml) dilution.

Panel A : merged staining of anti-ADX (gray; Opal™690) anti-VGF (green; Opal™520) and anti-Neuropeptide Y (magenta; Opal™570) on mouse adrenal gland.
Panel B : anti-ADX staining adrenal cortex in mouse adrenal gland.
Panel C : anti-VGF staining endocrine cells in mouse adrenal gland medulla.
Panel D : nti-Neuropeptide Y staining chromaffin cells in mouse adrenal gland medulla.
Nuclear DNA was labelled with DAPI (shown in blue). ).

The section was incubated in three rounds of staining : in the order of ab321899 ab308287 and ab221145 for 30 mins at room temperature.. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ADX with ab321899 at 1/500 (0.976 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with ab321899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse adrenal gland tissue labeling ADX with ab321899 at 1/2000 (0.244 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse adrenal gland (A) at 1/500 dilution (0.976 ug/mL) and (B) at 1/2000 dilution (0.244 ug/mL). The section was incubated with ab321899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling ADX with ab321899 at 1/500 (0.976 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse testis. The section was incubated with ab321899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ADX with ab321899 at 1/500 (0.976 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse cerebrum. The section was incubated with ab321899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-ADX antibody [EPR29804-54] - BSA and Azide free (AB321900)

This data was developed using ab321899, the same antibody clone in a different buffer formulation.

Dot blot analysis of ADX using ab321899 at 1000 (0.488 ug/ml) followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1 : 100000 dilution.

Lane1 : His-tagged human ADX recombinant protein

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Dot Blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-ADX antibody [EPR29804-54] (<a href='/en-us/products/primary-antibodies/adx-antibody-epr29804-54-ab321899'>ab321899</a>) at 1/1000 dilution

All lanes:

His-tagged human ADX recombinant protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s