AB108257

Anti-ADX antibody [EPR4629]

Name: Anti-ADX antibody [EPR4629] (ab108257) | Abcam
Brand: Abcam Limited
SKU: AB108257
Price: 335 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(32 Publications)

Anti-ADX antibody [EPR4629] (ab108257) is a rabbit monoclonal antibody detecting ADX in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ADX, FDX1, Adrenal ferredoxin, Ferredoxin-1, Hepatoredoxin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (AB108257)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (AB108257)

Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma epithelial) cells with ab108257 at a 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Mouse anti-COX IV monoclonal antibody - Mitochondrial Marker was used as a counterstain at a 1/50 dilution followed by ab150115 Alexa Fluor^® 647 Goat anti-Mouse secondary antibody used at a 1/1000 dilution.

The negative controls are shown in bottom middle and right hand panels - for negative control 1, ab108257 was used at a dilution of 1/100 followed by Alexa Fluor^® 647 Goat anti-Mouse secondary ab150115 at a dilution of 1/1000. For negative control 2, Mouse anti-COX IV monoclonal antibody - Mitochondrial Marker was used at a dilution of 1/50 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed ab150081 at a dilution of 1/1000.

Confocal image showing cytoplasmic staining in A549 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Western blot - Anti-ADX antibody [EPR4629] (AB108257)

All lanes:

Western blot - Anti-ADX antibody [EPR4629] (ab108257) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) whole cell lysate

Lane 2:

Mouse kidney lysate

Lane 3:

Rat kidney lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 19 kDa

false

Carrier free

Anti-ADX antibody [EPR4629] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4629

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval is recommended." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval is recommended." }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval is recommended." } } }

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Product details

What is this antibody validated in?
Anti-ADX antibody [EPR4629] (ab108257) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of ADX?
Anti-ADX [EPR4629] (ab108257) specifically detects a band for ADX (UniProt: P10109) at a molecular weight of 19kDa.

Trusted by the scientific community
Anti-ADX [EPR4629] (ab108257) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR4629] also available for your convenience: ab108257, Carrier free - ab247613

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alternate names for ADX include ADX mouse and FDX1. This target is a protein with a mass of approximately 54 kDa. ADX is expressed in various tissues with a high presence in adrenal cortex and testes. Mechanically it functions as an adrenodoxin which transfers electrons in the adrenal gland mitochondria. As a small iron-sulfur protein ADX interacts with cytochrome P450 enzymes to facilitate steroidogenic reactions.

Biological function summary

ADX plays a significant role in enabling steroid biosynthesis and metabolism acting as an electron donor. ADX is an essential component of the mitochondrial cholesterol side-chain cleavage complex. This complex helps in converting cholesterol into pregnenolone initiating steroid hormone production. It is necessary for producing corticosteroids androgens and estrogens.

Pathways

ADX holds critical functions in the cholesterol and steroid biosynthesis pathways. ADX interacts with the cholesterol transport protein StAR and cytochrome P450scc both essential for initiating the conversion of cholesterol to pregnenolone. Additionally it supports the electron transfer processes necessary for the final conversion steps of various steroid hormones.

Abnormal ADX functioning connects to disorders like congenital adrenal hyperplasia and Addison's disease. These conditions are often linked with insufficient steroid hormone production. Furthermore defects or imbalances in the ADX pathway may involve related proteins such as cytochrome P450 enzymes which could lead to disrupted steroidogenesis and further complications in adrenal gland function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the synthesis of various steroid hormones (PubMed : 20547883, PubMed : 21636783). Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis (PubMed : 20547883, PubMed : 21636783). Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (PubMed : 20547883, PubMed : 21636783). Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons (By similarity).

See full target information FDX1

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

BMC geriatrics 25:645 PubMed40836274

2025

AIMP1 exerts hearing protection role in age related hearing loss mice by regulating SIRT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haisen Peng,Jiali Liu,Yuehui Liu,Chunhua Li,Zhilin Zhang,Shuihua Hu,Wen Xie

Discover oncology 16:1521 PubMed40788438

2025

Clinacanthus nutans (Burm.f.) Lindau facilitates cuproptosis and ameliorates colon cancer progression by inhibiting PDE3B-mediated Apelin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gaoyun Zhou,Xueying Lin,Zhiheng Lai,Dewen Su,Long Lin,Xuewu Chen

Nature communications 16:7347 PubMed40783561

2025

Ligand supplementation restores the cancer therapy efficacy of the antirheumatic drug auranofin from serum inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Wang,Bei Cao,Qianqian Wang,Sinan Zhong,Xin Fang,Junjian Wang,Albert S C Chan,Xiaolin Xiong,Taotao Zou

MedComm 6:e70312 PubMed40761482

2025

Plumbagin Triggers Cuproptosis in Hepatocellular Carcinoma (HCC) via the DNA-Methyltransferase 1 (DNMT1)/microRNA-302a-3p (miR-302a-3p)/ATPase Copper Transporting Beta (ATP7B) Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chuyu Wang,Hao Wang,Chong Wang,Tongtong Tian,Anli Jin,Yu Liu,Ran Huo,Te Liu,Baishen Pan,Wei Guo,Wenjing Yang,Beili Wang

British journal of pharmacology 182:2409-2425 PubMed39933533

2025

Targeting FDX1 by trilobatin to inhibit cuproptosis in doxorubicin-induced cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Wei,Guozhen Lan,Wenfang Zhang,Wang Ran,Yu Wei,Xin Liu,Yuandong Zhang,Qihai Gong,Haibo Li,Jianmei Gao

Polish journal of microbiology 73:535-539 PubMed39670641

2024

Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by .

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan An,Wenyi Ding

Frontiers in immunology 15:1481129 PubMed39654888

2024

IAPP blocks anti-breast cancer function of CD8T cells via targeting cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Guo,Zhijian Huang,Qianqian Wang,Wei Chen,Yu Huang,Xinhao Sun,Jian Chen,Shuying Feng

Discover oncology 15:706 PubMed39585552

2024

BIRC5 knockdown ameliorates hepatocellular carcinoma progression via regulating PPARγ pathway and cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxing Mai,Zhuocheng Ji,Yujing Tan,Lei Feng,Jiasheng Qin

The Journal of biological chemistry 300:107995 PubMed39547509

2024

Engineered bacterial lipoate protein ligase A (lplA) restores lipoylation in cell models of lipoylation deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Nolan R Bick,Margaret B Dreishpoon,Ava Perry,Anna Rogachevskaya,Sylvia S Bottomley,Mark D Fleming,Sarah Ducamp,Peter Tsvetkov

Journal of biochemical and molecular toxicology 38:e23848 PubMed39264832

2024

GAD1 ameliorates glioma progression through regulating cuproptosis via RAS/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Gao,Jing Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADX antibody [EPR4629]

Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (AB108257)

Western blot - Anti-ADX antibody [EPR4629] (AB108257)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

Product promise