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Anti-ADX antibody [EPR4629] (ab108257) is a rabbit monoclonal antibody that is used to detect ADX in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Western blot - Anti-ADX antibody [EPR4629] (AB108257), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (AB108257), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IFWBIHC-P
Human
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Expected
Tested
Not recommended
Rat
Not recommended
Expected
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Antigen retrieval is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Antigen retrieval is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Antigen retrieval is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

Essential for the synthesis of various steroid hormones (PubMed:20547883, PubMed:21636783). Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis (PubMed:20547883, PubMed:21636783). Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (PubMed:20547883, PubMed:21636783). Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons (By similarity).

Alternative names

ADX, FDX1, Adrenal ferredoxin, Ferredoxin-1, Hepatoredoxin

Recommended products

Anti-ADX antibody [EPR4629] (ab108257) is a rabbit monoclonal antibody that is used to detect ADX in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4629
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-ADX antibody [EPR4629] (ab108257) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of ADX?


Anti-ADX [EPR4629] (ab108257) specifically detects a band for ADX (UniProt: P10109) at a molecular weight of 19kDa.

Trusted by the scientific community


Anti-ADX [EPR4629] (ab108257) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR4629] also available for your convenience:
ab108257, Carrier free - Anti-ADX antibody [EPR4629] - BSA and Azide free ab247613



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alternate names for ADX include ADX mouse and FDX1. This target is a protein with a mass of approximately 54 kDa. ADX is expressed in various tissues with a high presence in adrenal cortex and testes. Mechanically it functions as an adrenodoxin which transfers electrons in the adrenal gland mitochondria. As a small iron-sulfur protein ADX interacts with cytochrome P450 enzymes to facilitate steroidogenic reactions.

Biological function summary

ADX plays a significant role in enabling steroid biosynthesis and metabolism acting as an electron donor. ADX is an essential component of the mitochondrial cholesterol side-chain cleavage complex. This complex helps in converting cholesterol into pregnenolone initiating steroid hormone production. It is necessary for producing corticosteroids androgens and estrogens.

Pathways

ADX holds critical functions in the cholesterol and steroid biosynthesis pathways. ADX interacts with the cholesterol transport protein StAR and cytochrome P450scc both essential for initiating the conversion of cholesterol to pregnenolone. Additionally it supports the electron transfer processes necessary for the final conversion steps of various steroid hormones.

Associated diseases and disorders

Abnormal ADX functioning connects to disorders like congenital adrenal hyperplasia and Addison's disease. These conditions are often linked with insufficient steroid hormone production. Furthermore defects or imbalances in the ADX pathway may involve related proteins such as cytochrome P450 enzymes which could lead to disrupted steroidogenesis and further complications in adrenal gland function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-ADX antibody [EPR4629] (ab108257), expandable thumbnail

    Western blot - Anti-ADX antibody [EPR4629] (ab108257)

    All lanes: Western blot - Anti-ADX antibody [EPR4629] (ab108257) at 1/1000 dilution

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate

    Lane 2: Mouse kidney lysate

    Lane 3: Rat kidney lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 19 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (ab108257), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (ab108257)

    Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma epithelial) cells with ab108257 at a 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Mouse anti-COX IV monoclonal antibody - Mitochondrial Marker was used as a counterstain at a 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150115 Alexa Fluor®,/sup> 647 Goat anti-Mouse secondary antibody used at a 1/1000 dilution.

    The negative controls are shown in bottom middle and right hand panels - for negative control 1, ab108257 was used at a dilution of 1/100 followed by Alexa Fluor® 647 Goat anti-Mouse secondary Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150115 at a dilution of 1/1000. For negative control 2, Mouse anti-COX IV monoclonal antibody - Mitochondrial Marker was used at a dilution of 1/50 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a dilution of 1/1000.

    Confocal image showing cytoplasmic staining in A549 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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