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AB249451

Anti-AE binding protein 1 antibody [EPR7733(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal AEBP1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ACLP, AEBP1, Adipocyte enhancer-binding protein 1, AE-binding protein 1, Aortic carboxypeptidase-like protein

1 Images
Western blot - Anti-AE binding protein 1 antibody [EPR7733(2)] - BSA and Azide free (AB249451)
  • WB

Unknown

Western blot - Anti-AE binding protein 1 antibody [EPR7733(2)] - BSA and Azide free (AB249451)

This data was developed using ab168355, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AE binding protein 1 antibody [EPR7733(2)] (<a href='/en-us/products/primary-antibodies/ae-binding-protein-1-antibody-epr77332-ab168355'>ab168355</a>) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 131 kDa

false

  • Unconjugated

    Anti-AE binding protein 1 antibody [EPR7733(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7733(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249451 is the carrier-free version of ab168355.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AE binding protein 1 also known as AEBP1 functions mechanically as a transcriptional repressor. It interacts with other proteins to alter gene expression. The protein has a molecular mass of approximately 150 kDa. Researchers find AEBP1 in various tissues but expression is high in adipose tissue and the uterus. The protein's role as a repressor links to its ability to modulate the activities of certain transcription factors influencing cellular processes.
Biological function summary

AEBP1 plays a role in regulating the expression of genes important for adipogenesis and inflammation. It acts as part of a transcriptional regulation complex that controls genes driving fat cell development and lipid metabolism. Beyond adipose tissue AEBP1 also influences inflammatory responses which may connect to its function in modulating the immune system. The protein's ability to interact with intracellular pathways points to its involvement in cellular functions critical for maintaining homeostasis.

Pathways

AEBP1 integrates into the PPARγ signaling pathway. This pathway is important for adipocyte differentiation and lipid homeostasis. AEBP1's interaction with PPARγ connects it to the regulation of adipogenesis and metabolism. AEBP1 also participates indirectly in pathways modulating inflammatory responses. Through these connections AEBP1 influences proteins like C/EBPα which are key regulators of adipocyte commitment and growth.

AEBP1 has associations with obesity and inflammatory disorders. Its role in adipogenesis and inflammation links AEBP1 to obesity-related conditions where altered expression may contribute to weight gain and metabolic imbalances. Inflammatory disorders also involve AEBP1 as its modulation of immune-related factors can exacerbate or mitigate inflammation. Researchers recognize connections between AEBP1 and other proteins involved in these diseases such as NF-κB where AEBP1's regulation of inflammatory pathways could impact the progression of inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. As a positive regulator of collagen fibrillogenesis, it is probably involved in the organization and remodeling of the extracellular matrix.. Isoform 2. May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor.
See full target information AEBP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com