Rabbit Polyclonal AE2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC4A2 aa 1-50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC4A2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P04920
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane (PubMed:15184086, PubMed:34668226). Plays an important role in osteoclast differentiation and function (PubMed:34668226). Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH (By similarity). Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses (By similarity).
Alternative names
AE2, EPB3L1, HKB3, MPB3L, SLC4A2, Anion exchange protein 2, AE 2, Anion exchanger 2, Non-erythroid band 3-like protein, Solute carrier family 4 member 2, BND3L
Recommended products
Rabbit Polyclonal AE2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC4A2 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC4A2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P04920
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab226829 was affinity purified using an epitope specific to AE2 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The AE2 protein also known as solute carrier family 4 member 2 (SLC4A2) functions as an anion exchanger. It plays an important role in transporting chloride and bicarbonate across the cell membrane. AE2 typically weighs around 150 kDa. This protein is expressed in various tissues with notable expression patterns in liver kidney and gastric tissues. AE2 also localizes in the apical membrane of epithelial cells and plays a critical role in pH regulation and electrolyte balance.
- Biological function summary
AE2 is essential for maintaining the acid-base balance within cells. It functions not just on its own but also as part of larger protein complexes that interact with cytoskeletal and signaling proteins. Given its role in pH regulation AE2 has significant implications in cellular homeostasis and signal transduction. Its expression in marmoset breast tissue and other epithelial structures supports its function in regulating fluid and ion exchange in various organ systems.
- Pathways
Scientists identified AE2 as an important component in the bicarbonate transport pathway and epithelial ion transport pathway. It interacts with various proteins including carbonic anhydrase to facilitate bicarbonate reabsorption and secretion. The pathways involving AE2 contribute to maintaining systemic pH and are critical in processes such as urine acidification and pancreatic secretion. AE2 also has functional links with proteins responsible for ion transport dictating fluid secretion and absorption.
- Associated diseases and disorders
Researchers have linked AE2 to conditions such as primary biliary cholangitis and gastric disorders related to acid-base imbalance. In these diseases disruptions in AE2 function can lead to alterations in bicarbonate transport causing symptom exacerbation. AntiAE antibodies targeting AE2 are explored as therapeutic options or diagnostic markers in managing these diseases. Through these mechanisms AE2 maintains a strong connection with proteins involved in pH regulation and immune response modulation affecting disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody (ab226829)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for AE2 using ab226829 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
Western blot - Anti-AE2 antibody (ab226829)
Lysates were prepared using RIPA lysis buffer.
All lanes: Western blot - Anti-AE2 antibody (ab226829) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 30s
Immunoprecipitation - Anti-AE2 antibody (ab226829)
AE2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226829 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226829 at 1 μg/ml.
Lane 1: ab226829 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-AE2 antibody (ab226829)
Predicted band size: 137 kDa
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