JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB226829

Anti-AE2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal AE2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC4A2 aa 1-50.

View Alternative Names

AE2, EPB3L1, HKB3, MPB3L, SLC4A2, Anion exchange protein 2, AE 2, Anion exchanger 2, Non-erythroid band 3-like protein, Solute carrier family 4 member 2, BND3L

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody (AB226829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody (AB226829)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for AE2 using ab226829 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB staining.

Immunoprecipitation - Anti-AE2 antibody (AB226829)
  • IP

Supplier Data

Immunoprecipitation - Anti-AE2 antibody (AB226829)

AE2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226829 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226829 at 1 μg/ml.

Lane 1 : ab226829 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-AE2 antibody (ab226829)

Predicted band size: 137 kDa

false

Western blot - Anti-AE2 antibody (AB226829)
  • WB

Supplier Data

Western blot - Anti-AE2 antibody (AB226829)

Lysates were prepared using RIPA lysis buffer.

All lanes:

Western blot - Anti-AE2 antibody (ab226829) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 137 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SLC4A2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P04920

style
left-column

Alternative Products

Primary Antibodies

AB140953

Anti-AE2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (AB140953)

  • 1
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

  • 5
  • 2
Primary Antibodies

AB131553

Anti-CFTR antibody

Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (AB131553)

  • 5
  • 1
Primary Antibodies

AB75850

Anti-LGR5 antibody [EPR3065Y]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

  • 4
  • 7
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab226829 was affinity purified using an epitope specific to AE2 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AE2 protein also known as solute carrier family 4 member 2 (SLC4A2) functions as an anion exchanger. It plays an important role in transporting chloride and bicarbonate across the cell membrane. AE2 typically weighs around 150 kDa. This protein is expressed in various tissues with notable expression patterns in liver kidney and gastric tissues. AE2 also localizes in the apical membrane of epithelial cells and plays a critical role in pH regulation and electrolyte balance.
Biological function summary

AE2 is essential for maintaining the acid-base balance within cells. It functions not just on its own but also as part of larger protein complexes that interact with cytoskeletal and signaling proteins. Given its role in pH regulation AE2 has significant implications in cellular homeostasis and signal transduction. Its expression in marmoset breast tissue and other epithelial structures supports its function in regulating fluid and ion exchange in various organ systems.

Pathways

Scientists identified AE2 as an important component in the bicarbonate transport pathway and epithelial ion transport pathway. It interacts with various proteins including carbonic anhydrase to facilitate bicarbonate reabsorption and secretion. The pathways involving AE2 contribute to maintaining systemic pH and are critical in processes such as urine acidification and pancreatic secretion. AE2 also has functional links with proteins responsible for ion transport dictating fluid secretion and absorption.

Researchers have linked AE2 to conditions such as primary biliary cholangitis and gastric disorders related to acid-base imbalance. In these diseases disruptions in AE2 function can lead to alterations in bicarbonate transport causing symptom exacerbation. AntiAE antibodies targeting AE2 are explored as therapeutic options or diagnostic markers in managing these diseases. Through these mechanisms AE2 maintains a strong connection with proteins involved in pH regulation and immune response modulation affecting disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane (PubMed : 15184086, PubMed : 34668226). Plays an important role in osteoclast differentiation and function (PubMed : 34668226). Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH (By similarity). Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses (By similarity).
See full target information SLC4A2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 46:675-684 PubMed32626954

2020

Paeonol exerts anti‑tumor activity against colorectal cancer cells by inducing G0/G1 phase arrest and cell apoptosis via inhibiting the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Hua Liu,Ren-Jie Shi,Zhi-Cheng Chen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com