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Rabbit Polyclonal AE2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC4A2.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human SLC4A2. The exact immunogen used to generate this antibody is proprietary information. Database link P04920
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Predicted
Rat
Predicted
Chimpanzee
Predicted
Common marmoset
Predicted
Cow
Predicted
Dog
Predicted
Guinea pig
Predicted
Hamster
Predicted
Horse
Predicted
Monkey
Predicted
Orangutan
Predicted
Pig
Predicted
Rabbit
Predicted

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Monkey, Common marmoset, Orangutan

Dilution info

-

Notes

-

Target data

Function

Plasma membrane anion exchange protein of wide distribution.

Alternative names

Recommended products

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Rabbit Polyclonal AE2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC4A2.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human SLC4A2. The exact immunogen used to generate this antibody is proprietary information. Database link P04920
Purification technique

Affinity purification Immunogen

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

AE2 is essential for maintaining the acid-base balance within cells. It functions not just on its own but also as part of larger protein complexes that interact with cytoskeletal and signaling proteins. Given its role in pH regulation AE2 has significant implications in cellular homeostasis and signal transduction. Its expression in marmoset breast tissue and other epithelial structures supports its function in regulating fluid and ion exchange in various organ systems.

Activity summary

The AE2 protein also known as solute carrier family 4 member 2 (SLC4A2) functions as an anion exchanger. It plays an important role in transporting chloride and bicarbonate across the cell membrane. AE2 typically weighs around 150 kDa. This protein is expressed in various tissues with notable expression patterns in liver kidney and gastric tissues. AE2 also localizes in the apical membrane of epithelial cells and plays a critical role in pH regulation and electrolyte balance.

Pathways

Scientists identified AE2 as an important component in the bicarbonate transport pathway and epithelial ion transport pathway. It interacts with various proteins including carbonic anhydrase to facilitate bicarbonate reabsorption and secretion. The pathways involving AE2 contribute to maintaining systemic pH and are critical in processes such as urine acidification and pancreatic secretion. AE2 also has functional links with proteins responsible for ion transport dictating fluid secretion and absorption.

Associated diseases and disorders

Researchers have linked AE2 to conditions such as primary biliary cholangitis and gastric disorders related to acid-base imbalance. In these diseases disruptions in AE2 function can lead to alterations in bicarbonate transport causing symptom exacerbation. AntiAE antibodies targeting AE2 are explored as therapeutic options or diagnostic markers in managing these diseases. Through these mechanisms AE2 maintains a strong connection with proteins involved in pH regulation and immune response modulation affecting disease progression.

Product promise

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Full details and terms and conditions can be found here:
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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling AE2 with ab140953 at 10 μg/ml after heat-induced antigen retrieval.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labelling AE2 with ab140953 at 10 µg/ml after heat-induced antigen retrieval.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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