Rabbit Polyclonal AE2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC4A2.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC4A2. The exact immunogen used to generate this antibody is proprietary information. Database link P04920
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Chimpanzee | Predicted |
| Common marmoset | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Guinea pig | Predicted |
| Hamster | Predicted |
| Horse | Predicted |
| Monkey | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Monkey, Common marmoset, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane (PubMed:15184086, PubMed:34668226). Plays an important role in osteoclast differentiation and function (PubMed:34668226). Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH (By similarity). Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses (By similarity).
Alternative names
AE2, EPB3L1, HKB3, MPB3L, SLC4A2, Anion exchange protein 2, AE 2, Anion exchanger 2, Non-erythroid band 3-like protein, Solute carrier family 4 member 2, BND3L
Recommended products
Rabbit Polyclonal AE2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SLC4A2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC4A2. The exact immunogen used to generate this antibody is proprietary information. Database link P04920
- Purification technique
- Affinity purification Immunogen
- Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The AE2 protein also known as solute carrier family 4 member 2 (SLC4A2) functions as an anion exchanger. It plays an important role in transporting chloride and bicarbonate across the cell membrane. AE2 typically weighs around 150 kDa. This protein is expressed in various tissues with notable expression patterns in liver kidney and gastric tissues. AE2 also localizes in the apical membrane of epithelial cells and plays a critical role in pH regulation and electrolyte balance.
- Biological function summary
AE2 is essential for maintaining the acid-base balance within cells. It functions not just on its own but also as part of larger protein complexes that interact with cytoskeletal and signaling proteins. Given its role in pH regulation AE2 has significant implications in cellular homeostasis and signal transduction. Its expression in marmoset breast tissue and other epithelial structures supports its function in regulating fluid and ion exchange in various organ systems.
- Pathways
Scientists identified AE2 as an important component in the bicarbonate transport pathway and epithelial ion transport pathway. It interacts with various proteins including carbonic anhydrase to facilitate bicarbonate reabsorption and secretion. The pathways involving AE2 contribute to maintaining systemic pH and are critical in processes such as urine acidification and pancreatic secretion. AE2 also has functional links with proteins responsible for ion transport dictating fluid secretion and absorption.
- Associated diseases and disorders
Researchers have linked AE2 to conditions such as primary biliary cholangitis and gastric disorders related to acid-base imbalance. In these diseases disruptions in AE2 function can lead to alterations in bicarbonate transport causing symptom exacerbation. AntiAE antibodies targeting AE2 are explored as therapeutic options or diagnostic markers in managing these diseases. Through these mechanisms AE2 maintains a strong connection with proteins involved in pH regulation and immune response modulation affecting disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling AE2 with ab140953 at 10 μg/ml after heat-induced antigen retrieval.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labelling AE2 with ab140953 at 10 µg/ml after heat-induced antigen retrieval.
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