AB140953

Anti-AE2 antibody - N-terminal

Name: Anti-AE2 antibody - N-terminal (ab140953) | Abcam
Brand: Abcam Limited
SKU: AB140953
Price: 630 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal AE2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLC4A2.

View Alternative Names

AE2, EPB3L1, HKB3, MPB3L, SLC4A2, Anion exchange protein 2, AE 2, Anion exchanger 2, Non-erythroid band 3-like protein, Solute carrier family 4 member 2, BND3L

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (AB140953)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (AB140953)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labelling AE2 with ab140953 at 10 µg/ml after heat-induced antigen retrieval.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (AB140953)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling AE2 with ab140953 at 10 μg/ml after heat-induced antigen retrieval.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC4A2. The exact immunogen used to generate this antibody is proprietary information.

P04920

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AE2 protein also known as solute carrier family 4 member 2 (SLC4A2) functions as an anion exchanger. It plays an important role in transporting chloride and bicarbonate across the cell membrane. AE2 typically weighs around 150 kDa. This protein is expressed in various tissues with notable expression patterns in liver kidney and gastric tissues. AE2 also localizes in the apical membrane of epithelial cells and plays a critical role in pH regulation and electrolyte balance.

Biological function summary

AE2 is essential for maintaining the acid-base balance within cells. It functions not just on its own but also as part of larger protein complexes that interact with cytoskeletal and signaling proteins. Given its role in pH regulation AE2 has significant implications in cellular homeostasis and signal transduction. Its expression in marmoset breast tissue and other epithelial structures supports its function in regulating fluid and ion exchange in various organ systems.

Pathways

Scientists identified AE2 as an important component in the bicarbonate transport pathway and epithelial ion transport pathway. It interacts with various proteins including carbonic anhydrase to facilitate bicarbonate reabsorption and secretion. The pathways involving AE2 contribute to maintaining systemic pH and are critical in processes such as urine acidification and pancreatic secretion. AE2 also has functional links with proteins responsible for ion transport dictating fluid secretion and absorption.

Researchers have linked AE2 to conditions such as primary biliary cholangitis and gastric disorders related to acid-base imbalance. In these diseases disruptions in AE2 function can lead to alterations in bicarbonate transport causing symptom exacerbation. AntiAE antibodies targeting AE2 are explored as therapeutic options or diagnostic markers in managing these diseases. Through these mechanisms AE2 maintains a strong connection with proteins involved in pH regulation and immune response modulation affecting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane (PubMed : 15184086, PubMed : 34668226). Plays an important role in osteoclast differentiation and function (PubMed : 34668226). Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH (By similarity). Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses (By similarity).

See full target information SLC4A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 29:355-371.e10 PubMed35245467

2022

Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary disease.

Applications

Unspecified application

Species

Unspecified reactive species

John M Hallett,Sofia Ferreira-Gonzalez,Tak Yung Man,Alastair M Kilpatrick,Hannah Esser,Kayleigh Thirlwell,Mark T Macmillan,Daniel Rodrigo-Torres,Benjamin J Dwyer,Victoria L Gadd,Candice Ashmore-Harris,Wei-Yu Lu,John P Thomson,Maurits A Jansen,Eoghan O'Duibhir,Philip J Starkey Lewis,Lara Campana,Rhona E Aird,Thomas S R Bate,Alasdair R Fraser,John D M Campbell,Gabriel C Oniscu,David C Hay,Anthony Callanan,Stuart J Forbes

Journal of Cancer 11:4250-4260 PubMed32368308

2020

IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape .

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Qian Hu,Bo-Wen Lei,Duo Wen,Ben Ma,Ting-Ting Zhang,Zhong-Wu Lu,Wen-Jun Wei,Yu-Long Wang,Yu Wang,Duan-Shu Li,Qing-Hai Ji,Tian Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-AE2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (AB140953)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (AB140953)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise