AB208016

Anti-AF10 antibody [EPR20810]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal AF10 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

AF10, MLLT10, Protein AF-10, ALL1-fused gene from chromosome 10 protein

5 Images

Flow Cytometry (Intracellular) - Anti-AF10 antibody [EPR20810] (AB208016)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AF10 antibody [EPR20810] (AB208016)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling AF10 with ab208016 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (AB208016)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (AB208016)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human testis (PMID : 7888665; PMID : 11756182). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using Tris/EDTA buffer, pH 9.0.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (AB208016)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat testis (PMID : 7888665; PMID : 11756182). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using Tris/EDTA buffer, pH 9.0.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (AB208016)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling AF10 with ab208016 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse testis (PMID : 7888665; PMID : 11756182). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using Tris/EDTA buffer, pH 9.0.

Supplier Data

Western blot - Anti-AF10 antibody [EPR20810] (AB208016)

Exposure time : Lanes 1-2 : 3 minutes; Lane 3 : 5 seconds; Lane 4 : 15 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument

Lanes 1 - 3:

Western blot - Anti-AF10 antibody [EPR20810] (ab208016) at 1/5000 dilution

Lane 4:

Western blot - Anti-AF10 antibody [EPR20810] (ab208016) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 2:

ES-D3 (mouse embryonic multipotent stem cell line) whole cell lysate at 20 µg

Lane 3:

Mouse testis lysate at 20 µg

Lane 4:

Rat testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 113 kDa

Observed band size: 130 kDa

true

Carrier free

Anti-AF10 antibody [EPR20810] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20810

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AF10 protein also known as MLLT10 acts mechanically by associating with chromatin to regulate gene expression. The protein weighs approximately 225 kDa. Scientists find AF10 in a variety of tissues prominently in the nucleus where it influences gene regulation through interaction with chromatin structures. As a member of the AF family of proteins AF10 has a basic leucine zipper (bZIP) domain which facilitates its role in DNA binding and transcription regulation.

Biological function summary

AF10 participates in the regulation of gene transcription as part of larger protein complexes. It interacts with other chromatin-associated proteins and contributes to histone modification processes thereby affecting chromatin architecture and transcriptional activity. This binding to chromatin-modifying factors allows AF10 to maintain vital roles in controlling essential genes involved in cell cycle progression and differentiation.

Pathways

AF10 operates primarily in transcription regulation pathways and is linked to the HOX gene regulation pathway. It closely interacts with proteins such as MLL (Mixed-Lineage Leukemia) in the context of these pathways. Through its function in the transcription machinery AF10 facilitates the precise expression of gene sets necessary for proper cell lineage specification and development.

Malfunctions in AF10 are linked to acute leukemias such as acute myeloid leukemia (AML) due to aberrant gene translocations involving the MLL gene. This aberration can result in the creation of MLL-AF10 fusion proteins which contribute to oncogenic processes. Furthermore irregular AF10 expression or mutation can be connected with other hematological disorders impacting hematopoiesis and leading to disease progression. Researchers study its connection with MLL in a bid to develop targeted therapies aimed at modulating these leukemic transformations.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation (PubMed : 26439302).

See full target information MLLT10

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e56492 PubMed37317657

2023

DOT1L bridges transcription and heterochromatin formation at mammalian pericentromeres.

Applications

Species

Mouse

Aushaq B Malla,Haoming Yu,Delaney Farris,Srilekha Kadimi,TuKiet T Lam,Andy L Cox,Zachary D Smith,Bluma J Lesch

Clinical and translational medicine 12:e944 PubMed35735113

2022

Lipid metabolism-related lncRNA SLC25A21-AS1 promotes the progression of oesophageal squamous cell carcinoma by regulating the NPM1/c-Myc axis and SLC25A21 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Liu,Chunxiang Li,Lingling Fang,Liyu Wang,Hengchang Liu,He Tian,Yujia Zheng,Tao Fan,Jie He

Cancer management and research 12:5749-5758 PubMed32765078

2020

miR-331-3p Inhibits Tumor Cell Proliferation, Metastasis, Invasion by Targeting MLLT10 in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Qing Tian,Jing Xia,Xin Zhang,Bao-Qin Gao,Wei Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AF10 antibody [EPR20810]

Flow Cytometry (Intracellular) - Anti-AF10 antibody [EPR20810] (AB208016)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (AB208016)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (AB208016)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody [EPR20810] (AB208016)

Western blot - Anti-AF10 antibody [EPR20810] (AB208016)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise