JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB262920

Anti-AF4 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal AF4 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human AFF1 aa 1000-1100.

View Alternative Names

AF4, FEL, MLLT2, PBM1, AFF1, AF4/FMR2 family member 1, ALL1-fused gene from chromosome 4 protein, Protein FEL, Proto-oncogene AF4, Protein AF-4

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-AF4 antibody (AB262920)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AF4 antibody (AB262920)

PFA-fixed, Triton X-100 permeabilized human SiHa cells stained for NULL (green) using ab262920 at 4 µg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human AFF1 aa 1000-1100. The exact immunogen used to generate this antibody is proprietary information.

P51825

style
left-column

Complementary Products

Primary Antibodies

AB154492

Anti-AF9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF9 antibody (AB154492)

  • 3
  • 1
Primary Antibodies

AB96729

Anti-PCCB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

  • 0
  • 0
Primary Antibodies

AB254336

Anti-MTF2 antibody [EPR23536-87] - ChIP Grade

RabMAb|Recombinant

ChIP - Anti-MTF2 antibody [EPR23536-87] - ChIP Grade (AB254336)

  • 5
  • 2
Primary Antibodies

AB274415

Anti-MCH antibody [EPR23778-94]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

  • 5
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AF4 protein also known as MLLT2 is a molecular component with an approximate mass of 131 kDa. This protein is typically expressed in hematopoietic tissues where it plays a role in various cellular functions. The AF4 protein acts as a transcriptional activator and is part of the larger AF4/LAF4/FMR2 (ALF) family which plays significant roles in gene regulation. It participates in the transcriptional regulation process by modulating RNA polymerase II activity ensuring proper expression of specific genes important for cell function.
Biological function summary

AF4 influences transcriptional regulation by interacting with the super elongation complex (SEC). The SEC is known for enhancing gene transcription elongation. AF4 contributes to the proper transcription of genes involved in development and differentiation especially affecting the hematopoietic system where it facilitates normal cell differentiation processes. This involvement identifies AF4 as an integral player in maintaining cellular function and identity through its participation in the regulation of genetic transcription.

Pathways

AF4 contributes importantly to the Wnt and Hedgehog signaling pathways. These pathways are essential in controlling cell fate determination proliferation and migration. The protein's interaction with RNA polymerase II places it in a vital position to influence these pathways directly. Within these pathways AF4's functions are related to those of the other proteins like transcription factors TCF/LEF in Wnt signaling and Gli proteins in Hedgehog signaling highlighting its role in gene expression regulation within these cellular processes.

AF4 has been implicated in leukemias particularly in acute lymphoblastic leukemia (ALL) where chromosomal translocations often involve AF4. This translocation disrupts normal gene function leading to the proliferation of leukemic cells. The interaction between AF4 and the MLL protein resulting in the MLL-AF4 fusion protein contributes to oncogenic processes in these leukemias. Moreover abnormalities in AF4 function have been linked to certain neurological disorders though the specific mechanism and associated proteins require further investigation to be thoroughly understood.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information AFF1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com