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Mouse Monoclonal I Afadin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-AF6 antibody [35/AF6] (AB300635), expandable thumbnail
  • Western blot - Anti-AF6 antibody [35/AF6] (AB300635), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton (PubMed:11024295). May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions (PubMed:30463011).

Additional Targets

AFDN, AFDN, AFDN, AFDN, AFDN

Alternative names

AF6, MLLT4, AFDN, Afadin, ALL1-fused gene from chromosome 6 protein, Afadin adherens junction formation factor, Protein AF-6

Recommended products

Mouse Monoclonal I Afadin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
35/AF6 H1L1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

AF6 also known as Afadin or anti-i is a protein involved in cell signaling and adhesion mechanisms. It weighs approximately 180 kDa and plays a role in the formation of tight junctions influencing cellular architecture and communication. AF6 is expressed in a variety of tissues including epithelial cells indicating its involvement in maintaining tissue integrity. The protein localizes at cellular junctions where it interacts with other junctional components facilitating connections between adjacent cells.

Biological function summary

AF6 contributes to cell-to-cell contact by organizing cytoskeletal elements and signaling elements. It exists within the nectin-afadin complex which is critical for the regulation of tight junctions and adherens junctions. This positioning allows AF6 to modulate processes like cell migration and tissue morphogenesis. Through its interactions with actin filaments and other junctional proteins AF6 has a significant role in maintaining proper cellular organization and stability.

Pathways

AF6 participates actively in the Ras signaling and Rap1 pathways. Within the Ras pathway AF6 associates with proteins like Ras and Rap1 which regulate various cellular outcomes including proliferation and differentiation. AF6's involvement with these pathways enhances its role in influencing cell dynamics and signaling decisions. AF6's function in these signaling pathways underlines its participation in normal cell function and development.

Associated diseases and disorders

AF6 has links to cancer progression and neurological disorders such as autism. The protein’s interaction with Ras influences oncogenic signaling which can lead to aberrant cell growth and metastasis in cancers. Additionally disruptions in AF6 function or expression levels have associations with autism spectrum disorders as AF6 affects neural cell adhesion and signaling pathways. The protein’s interactions with junctional proteins like cadherins and nectins in diseased states suggest its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-AF6 antibody [35/AF6] (ab300635), expandable thumbnail

    Western blot - Anti-AF6 antibody [35/AF6] (ab300635)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were freshly made and used immediately to minimize protein degradation.

    The identity of the lower MW band at approximately 20kDa is unknown.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-AF6 antibody [35/AF6] (ab300635) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Observed band size: 207 kDa, 189 kDa, 190 kDa

    Exposure time: 3min

  • Western blot - Anti-AF6 antibody [35/AF6] (ab300635), expandable thumbnail

    Western blot - Anti-AF6 antibody [35/AF6] (ab300635)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: (PMID: 21478912 and PMID: 24269953)

    The lower bands in may be caused by degradation.

    Lysates were freshly made and used immediately to minimize protein degradation.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-AF6 antibody [35/AF6] (ab300635) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 3: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 4: T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Observed band size: 207 kDa, 190 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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