Anti-AF6 antibody [35/AF6] - BSA and Azide Free

• WB

Supplier Data

Western blot - Anti-AF6 antibody [35/AF6] - BSA and Azide Free (AB300636)

This data was developed using ab300635, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lysates were freshly made and used immediately to minimize protein degradation. The identity of the lower MW band at approximately 20kDa is unknown. Exposure time : 3 minutes

All lanes:

Western blot - Anti-AF6 antibody [35/AF6] (<a href='/en-us/products/primary-antibodies/af6-antibody-35-af6-ab300635'>ab300635</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Observed band size: 207 kDa,189 kDa,190 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-AF6 antibody [35/AF6] - BSA and Azide Free (AB300636)

This data was developed using ab300635, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Low expression : (PMID : 21478912 and PMID : 24269953)

The lower bands in may be caused by degradation.

Lysates were freshly made and used immediately to minimize protein degradation.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-AF6 antibody [35/AF6] (<a href='/en-us/products/primary-antibodies/af6-antibody-35-af6-ab300635'>ab300635</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 207 kDa,190 kDa

true

Exposure time: 3min