AB203569

Anti-AF6 antibody [A7L9H48]

Name: Anti-AF6 antibody [A7L9H48] (ab203569) | Abcam
Brand: Abcam Limited
SKU: AB203569
Price: 575 USD
Availability: InStock
Rating: 4 (1 reviews)

Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal I Afadin antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Afdn aa 1800 to C-terminus.

View Alternative Names

Af6, Mllt4, Afdn, Afadin, Afadin adherens junction formation factor, Protein Af-6

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF6 antibody [A7L9H48] (AB203569)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF6 antibody [A7L9H48] (AB203569)

Immunohistochemical analysis of Mouse hyppocampus tissue labeling l + S Afadin using ab203569 at 1 μg/ml.

Supplier Data

Western blot - Anti-AF6 antibody [A7L9H48] (AB203569)

All lanes:

Western blot - Anti-AF6 antibody [A7L9H48] (ab203569) at 1 µg/mL

All lanes:

Mouse hyppocampus tissue lysate (unboiled)

Predicted band size: 207 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

A7L9H48

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Mouse Afdn aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9QZQ1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>Do not boil samples.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AF6 also known as Afadin or anti-i is a protein involved in cell signaling and adhesion mechanisms. It weighs approximately 180 kDa and plays a role in the formation of tight junctions influencing cellular architecture and communication. AF6 is expressed in a variety of tissues including epithelial cells indicating its involvement in maintaining tissue integrity. The protein localizes at cellular junctions where it interacts with other junctional components facilitating connections between adjacent cells.

Biological function summary

AF6 contributes to cell-to-cell contact by organizing cytoskeletal elements and signaling elements. It exists within the nectin-afadin complex which is critical for the regulation of tight junctions and adherens junctions. This positioning allows AF6 to modulate processes like cell migration and tissue morphogenesis. Through its interactions with actin filaments and other junctional proteins AF6 has a significant role in maintaining proper cellular organization and stability.

Pathways

AF6 participates actively in the Ras signaling and Rap1 pathways. Within the Ras pathway AF6 associates with proteins like Ras and Rap1 which regulate various cellular outcomes including proliferation and differentiation. AF6's involvement with these pathways enhances its role in influencing cell dynamics and signaling decisions. AF6's function in these signaling pathways underlines its participation in normal cell function and development.

AF6 has links to cancer progression and neurological disorders such as autism. The protein’s interaction with Ras influences oncogenic signaling which can lead to aberrant cell growth and metastasis in cancers. Additionally disruptions in AF6 function or expression levels have associations with autism spectrum disorders as AF6 affects neural cell adhesion and signaling pathways. The protein’s interactions with junctional proteins like cadherins and nectins in diseased states suggest its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton (By similarity). May play a key role in the organization of epithelial structures of the embryonic ectoderm (PubMed : 10477764). Essential for the organization of adherens junctions (By similarity).

See full target information Afdn

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:4562 PubMed35931706

2022

Afadin couples RAS GTPases to the polarity rheostat Scribble.

Applications

Unspecified application

Species

Unspecified reactive species

Marilyn Goudreault,Valérie Gagné,Chang Hwa Jo,Swati Singh,Ryan C Killoran,Anne-Claude Gingras,Matthew J Smith

Cerebral cortex (New York, N.Y. : 1991) 27:1253-1269 PubMed26733533

2016

Rap1 GTPases Are Master Regulators of Neural Cell Polarity in the Developing Neocortex.

Applications

ICC/IF

Species

Mouse

Bhavin Shah,Daniela Lutter,Yaroslav Tsytsyura,Natalia Glyvuk,Akira Sakakibara,Jürgen Klingauf,Andreas W Püschel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AF6 antibody [A7L9H48]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF6 antibody [A7L9H48] (AB203569)

Western blot - Anti-AF6 antibody [A7L9H48] (AB203569)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise