Rabbit Polyclonal AF9 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MLLT3 aa 250-550.
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Publications
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- Nature 612:732-7382022Primate gastrulation and early organogenesis at single-cell resolution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinglei Zhai,Jing Guo,Haifeng Wan,Luqing Qi,Lizhong Liu,Zhenyu Xiao,Long Yan,Daniel A Schmitz,Yanhong Xu,Dainan Yu,Xulun Wu,Wentao Zhao,Kunyuan Yu,Xiangxiang Jiang,Fan Guo,Jun Wu,Hongmei WangPubMed 36517595
- The Journal of experimental medicine 216:2653-26682019Yeats4 drives ILC lineage commitment via activation of transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenyu Liu et. al.PubMed 31434684
- Transcription 8:133-1492017Super elongation complex promotes early HIV transcription and its function is modulated by P-TEFb.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlona Kuzmina et. al.PubMed 28340332
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MLLT3 aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link P42568
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Target data
Function
Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA (PubMed:20159561, PubMed:20471948, PubMed:25417107, PubMed:27105114, PubMed:27545619). Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated (PubMed:25417107, PubMed:27105114, PubMed:27545619, PubMed:30374167, PubMed:30385749). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:25417107, PubMed:27105114, PubMed:27545619). Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr) (PubMed:27105114). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3 (PubMed:25417107, PubMed:27105114, PubMed:30385749). In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones (PubMed:27105114, PubMed:27545619). Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 'Lys-79' methylation (H3K79me) (PubMed:25417107). Plays a key role in hematopoietic stem cell (HSC) maintenance by preserving, rather than conferring, HSC stemness (PubMed:31776511). Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L (PubMed:31776511).
Alternative names
AF9, YEATS3, MLLT3, Protein AF-9, ALL1-fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, YEATS domain-containing protein 3
Recommended products
Rabbit Polyclonal AF9 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MLLT3 aa 250-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MLLT3 aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link P42568
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The AF9 protein also known as AF-9 or AFNINE mechanically functions as a transcriptional coactivator involved in the regulation of gene expression. It has a molecular mass of approximately 62 kDa. AF9 expresses in various tissues including hematopoietic cells and plays an essential role in cellular transcription processes. The protein contains important domains that facilitate its interactions enabling it to modulate transcription by binding with other regulatory proteins.
- Biological function summary
Functions related to AF9 involve its inclusion in the super elongation complex (SEC) which is important for transcriptional elongation. This complex controls the transition of RNA polymerase II into productive elongation stages during transcription. AF9 interacts with other proteins such as MLL ENL and P-TEFb within the SEC enhancing transcription of certain genes vital for cell proliferation and differentiation.
- Pathways
AF9 plays significant roles in pathways like the MLL fusion pathway and the transcriptional regulation pathway. These pathways are integral to normal cellular processes as well as oncogenic transformations. In these pathways AF9 functionally relates to proteins such as Cyclin T1 and CDK9 through interactions within the super elongation complex modulating the transcription of genes involved in cell growth and division.
- Associated diseases and disorders
AF9 has strong links to leukemia particularly mixed lineage leukemia (MLL) due to its involvement in chromosomal translocations that create oncogenic MLL-AF9 fusion proteins. These fusions lead to altered transcription programs driving leukemogenesis. AF9 also connects to other oncoproteins like ENL that participate in similar pathways associated with MLL-related leukemias highlighting its critical role in disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-AF9 antibody (ab154492)
7.5% SDS PAGE
All lanes: Western blot - Anti-AF9 antibody (ab154492) at 1/1000 dilution
All lanes: THP1 whole cell lysate at 30 µg
Predicted band size: 63 kDa
Immunocytochemistry/ Immunofluorescence - Anti-AF9 antibody (ab154492)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling AF9 using ab154492 at 1/500 dilution. Right panel costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF9 antibody (ab154492)
Immunohistochemical analysis of paraffin-embedded D54 xenograft , labeling AF9 using ab154492 at 1/750 dilution.
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