Rabbit Polyclonal AFAF/Equatorin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EQTN aa 200 to C-terminus.
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Publications
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- Cell research 25:851-632015Generation of fertile offspring from Kit(w)/Kit(wv) mice through differentiation of gene corrected nuclear transfer embryonic stem cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EQTN aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQ60
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Recommend PFA Fixation and Triton X-100 treatment. |
Target data
Function
Acrosomal membrane-anchored protein involved in the process of fertilization and in acrosome biogenesis.
Alternative names
AFAF, C9orf11, EQTN, Equatorin, Acrosome formation-associated factor
Recommended products
Rabbit Polyclonal AFAF/Equatorin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EQTN aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EQTN aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQ60
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AFAF also known as Equatorin is a protein that plays a role in the structural integrity of the sperm acrosome. It has a molecular weight of approximately 60 kDa. AFAF is predominantly expressed in the testes particularly localized at the equatorial segment of the spermatozoon which is essential for the proper function of sperm cells.
- Biological function summary
The functions of AFAF involve maintaining the proper architecture of sperm cells. This protein is an integral part of the acrosomal membrane complex a structure essential for fertilization. AFAF interacts with other proteins in the acrosomal matrix contributing to its strength and stability which aids the sperm in penetrating the ovum.
- Pathways
The role of AFAF aligns with reproductive and fertilization-related biological processes. It is a component of the acrosome reaction pathway important for the fusion of sperm with the egg membrane. The protein associates with acrosin and other proteolytic enzymes which facilitate the breakdown of the extracellular matrix of the egg. These interactions are vital for successful fertility and the continuation of genetic material to the offspring.
- Associated diseases and disorders
Improper function or expression of AFAF can lead to male infertility. Abnormalities in AFAF may disrupt the acrosome reaction resulting in impaired fertilization. The protein's malfunction can also relate to oligozoospermia a condition marked by a low sperm count. Moreover its interactions with proteins like zonadhesin are relevant in the context of these reproductive health issues highlighting the importance of AFAF in maintaining reproductive capability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFAF/Equatorin antibody (ab121461)
ab121461 at a 1/300 dilution staining AFAF/Equatorin in Human testis tissue (paraffin embedded) by immunohistochemistry.
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