Rabbit Polyclonal AFF4 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AFF4.
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Publications
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- Journal of hematology & oncology 15:412022A proteolysis-targeting chimera molecule selectively degrades ENL and inhibits malignant gene expression and tumor growth.
- Theranostics 11:8172-81842021Small-molecule inhibitor of AF9/ENL-DOT1L/AF4/AFF4 interactions suppresses malignant gene expression and tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangrui Wu,Shenyou Nie,Yuan Yao,Tong Huo,Xin Li,Xiaowei Wu,Jidong Zhao,Yi-Lun Lin,Yinjie Zhang,Qianxing Mo,Yongcheng SongPubMed 34373735
- Cancer management and research 12:5919-59292020Triptonide Modulates MAPK Signaling Pathways and Exerts Anticancer Effects via ER Stress-Mediated Apoptosis Induction in Human Osteosarcoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiyun Zheng et. al.PubMed 32765093
- Journal of cellular biochemistry 121:2534-25422019Long noncoding RNA ZFPM2-AS1 is involved in lung adenocarcinoma via miR-511-3p/AFF4 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Li,Jun Ge,Ye Yang,Bin Liu,Min Zheng,Rui ShiPubMed 31692047
- Nature chemical biology 14:163-1702017Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCalla M Olson,Baishan Jiang,Michael A Erb,Yanke Liang,Zainab M Doctor,Zinan Zhang,Tinghu Zhang,Nicholas Kwiatkowski,Myriam Boukhali,Jennifer L Green,Wilhelm Haas,Tyzoon Nomanbhoy,Eric S Fischer,Richard A Young,James E Bradner,Georg E Winter,Nathanael S GrayPubMed 29251720
- Bone research 5:170442017AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human AFF4. Database link Q9UHB7
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
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Target data
Function
Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
Alternative names
AF5Q31, MCEF, HSPC092, AFF4, AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Major CDK9 elongation factor-associated protein, Protein AF-5q31
Recommended products
Rabbit Polyclonal AFF4 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AFF4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human AFF4. Database link Q9UHB7
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The AFF4 protein also known as AF4/FMR2 family member 4 is a transcriptional cofactor with a molecular weight of approximately 140 kDa. It plays a role in regulating transcriptional elongation by RNA polymerase II. This protein shows expression across diverse tissues with higher levels in the brain and testis. AFF4 contains domains that enable interactions with other proteins facilitating its role in transcriptional regulation.
- Biological function summary
AFF4 functions as a component of the super elongation complex (SEC) which is essential for transcriptional elongation of specific genes. This complex ensures efficient transcription after the initiation phase. Through its association with SEC AFF4 influences the expression of genes involved in cellular differentiation and development. Its activity is particularly impactful on genes associated with oncogenesis and neural development affecting cellular growth and specialized cell formation.
- Pathways
The function of AFF4 integrates into the transcription elongation and gene regulation pathways. AFF4 interacts with proteins like ELL2 and CDK9 which are critical components of the positive transcription elongation factor b (P-TEFb) complex. Together these proteins regulate polymerase activity and gene expression fundamental in cell cycle control and response to growth signals.
- Associated diseases and disorders
AFF4 has connections to cancer and neurological disorders. Mutations or altered expressions of AFF4 relate to acute lymphoblastic leukemia where it may form fusion proteins that disrupt normal gene expression. In neurological contexts aberrant AFF4 function can contribute to disorders such as developmental delay. Through these diseases AFF4 interacts with disease-related proteins like MLL and AF9 involved in oncogenic transformations and neurological development respectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-AFF4 antibody (ab103586)
All lanes: Western blot - Anti-AFF4 antibody (ab103586) at 1/500 dilution
All lanes: Human liver tissue lysate at 50 µg
Predicted band size: 127 kDa
Western blot - Anti-AFF4 antibody (ab103586)
All lanes: Western blot - Anti-AFF4 antibody (ab103586) at 1/500 dilution
All lanes: Mouse liver tissue lysate at 50 µg
Predicted band size: 127 kDa
Western blot - Anti-AFF4 antibody (ab103586)
All lanes: Western blot - Anti-AFF4 antibody (ab103586) at 1/500 dilution
Lane 1: AFF4 transfected 293T cell line lysate at 25 µg
Lane 2: Non-transfected 293T cell line lysate at 25 µg
Predicted band size: 127 kDa, 36 kDa, 37 kDa, 41 kDa, 42 kDa, 50 kDa, 57 kDa
Observed band size: 36 kDa, 40 kDa
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