AB103586

Anti-AFF4 antibody

Name: Anti-AFF4 antibody (ab103586) | Abcam
Brand: Abcam Limited
SKU: AB103586
Price: 535 USD
Availability: InStock

(1 Review)

(7 Publications)

Rabbit Polyclonal AFF4 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AFF4.

View Alternative Names

AF5Q31, MCEF, HSPC092, AFF4, AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Major CDK9 elongation factor-associated protein, Protein AF-5q31

3 Images

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Western blot - Anti-AFF4 antibody (AB103586)

All lanes:

Western blot - Anti-AFF4 antibody (ab103586) at 1/500 dilution

All lanes:

Mouse liver tissue lysate at 50 µg

Predicted band size: 127 kDa

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Western blot - Anti-AFF4 antibody (AB103586)

All lanes:

Western blot - Anti-AFF4 antibody (ab103586) at 1/500 dilution

Lane 1:

AFF4 transfected 293T cell line lysate at 25 µg

Lane 2:

Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 127 kDa,36 kDa,37 kDa,41 kDa,42 kDa,50 kDa,57 kDa

Observed band size: 36 kDa,40 kDa

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Unknown

Western blot - Anti-AFF4 antibody (AB103586)

All lanes:

Western blot - Anti-AFF4 antibody (ab103586) at 1/500 dilution

All lanes:

Human liver tissue lysate at 50 µg

Predicted band size: 127 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human AFF4.

Q9UHB7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AFF4 protein also known as AF4/FMR2 family member 4 is a transcriptional cofactor with a molecular weight of approximately 140 kDa. It plays a role in regulating transcriptional elongation by RNA polymerase II. This protein shows expression across diverse tissues with higher levels in the brain and testis. AFF4 contains domains that enable interactions with other proteins facilitating its role in transcriptional regulation.

Biological function summary

AFF4 functions as a component of the super elongation complex (SEC) which is essential for transcriptional elongation of specific genes. This complex ensures efficient transcription after the initiation phase. Through its association with SEC AFF4 influences the expression of genes involved in cellular differentiation and development. Its activity is particularly impactful on genes associated with oncogenesis and neural development affecting cellular growth and specialized cell formation.

Pathways

The function of AFF4 integrates into the transcription elongation and gene regulation pathways. AFF4 interacts with proteins like ELL2 and CDK9 which are critical components of the positive transcription elongation factor b (P-TEFb) complex. Together these proteins regulate polymerase activity and gene expression fundamental in cell cycle control and response to growth signals.

AFF4 has connections to cancer and neurological disorders. Mutations or altered expressions of AFF4 relate to acute lymphoblastic leukemia where it may form fusion proteins that disrupt normal gene expression. In neurological contexts aberrant AFF4 function can contribute to disorders such as developmental delay. Through these diseases AFF4 interacts with disease-related proteins like MLL and AF9 involved in oncogenic transformations and neurological development respectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.

See full target information AFF4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:13815-13828 PubMed35706403

2022

Circular RNA hsa_circ_0057452 facilitates keloid progression by targeting the microRNA-1225-3p/AF4/FMR2 family member 4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Gao,Zhen Hu,Xiangming Zhang

Journal of hematology & oncology 15:41 PubMed35395864

2022

A proteolysis-targeting chimera molecule selectively degrades ENL and inhibits malignant gene expression and tumor growth.

Applications

Species

Mouse

Xin Li,Yuan Yao,Fangrui Wu,Yongcheng Song

Theranostics 11:8172-8184 PubMed34373735

2021

Small-molecule inhibitor of AF9/ENL-DOT1L/AF4/AFF4 interactions suppresses malignant gene expression and tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Fangrui Wu,Shenyou Nie,Yuan Yao,Tong Huo,Xin Li,Xiaowei Wu,Jidong Zhao,Yi-Lun Lin,Yinjie Zhang,Qianxing Mo,Yongcheng Song

Cancer management and research 12:5919-5929 PubMed32765093

2020

Triptonide Modulates MAPK Signaling Pathways and Exerts Anticancer Effects via ER Stress-Mediated Apoptosis Induction in Human Osteosarcoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liyun Zheng,Shiji Fang,Junguo Hui,Vinothkumar Rajamanickam,Minjiang Chen,Qiaoyou Weng,Xulu Wu,Zhongwei Zhao,Jiansong Ji

Journal of cellular biochemistry 121:2534-2542 PubMed31692047

2019

Long noncoding RNA ZFPM2-AS1 is involved in lung adenocarcinoma via miR-511-3p/AFF4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Li,Jun Ge,Ye Yang,Bin Liu,Min Zheng,Rui Shi

Nature chemical biology 14:163-170 PubMed29251720

2017

Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Calla M Olson,Baishan Jiang,Michael A Erb,Yanke Liang,Zainab M Doctor,Zinan Zhang,Tinghu Zhang,Nicholas Kwiatkowski,Myriam Boukhali,Jennifer L Green,Wilhelm Haas,Tyzoon Nomanbhoy,Eric S Fischer,Richard A Young,James E Bradner,Georg E Winter,Nathanael S Gray

Bone research 5:17044 PubMed28955517

2017

AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs.

Applications

Species

Human

Chen-Chen Zhou,Qiu-Chan Xiong,Xin-Xing Zhu,Wen Du,Peng Deng,Xiao-Bing Li,Yi-Zhou Jiang,Shu-Juan Zou,Cun-Yu Wang,Quan Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AFF4 antibody

Western blot - Anti-AFF4 antibody (AB103586)

Western blot - Anti-AFF4 antibody (AB103586)

Western blot - Anti-AFF4 antibody (AB103586)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise